scholarly journals Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

2018 ◽  
Vol 27 (9) ◽  
pp. 1181-1193 ◽  
Author(s):  
Katarzyna Jezierska-Wozniak ◽  
Seweryn Lipiński ◽  
Łukasz Grabarczyk ◽  
Monika Barczewska ◽  
Aleksandra Habich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Electric Field ◽  
Human Mesenchymal Stem Cells ◽  
Pulsed Electric Field

scholarly journals Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

2017 ◽  
Author(s):  
Katarzyna Jezierska-Wozniak ◽  
Seweryn Lipiński ◽  
Łukasz Grabarczyk ◽  
Monika Barczewska ◽  
Aleksandra Habich ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Electric Field ◽  
Human Mesenchymal Stem Cells ◽  
Pulsed Electric Field ◽  
Cellular Systems ◽  
Cellular Migration

AbstractThe objective of our study was to develop novel techniques for investigations of cell motility, and to assess whether the electric field of the therapeutic spinal cord stimulation system used in vivo contributes to the migration of human mesenchymal stem cells (hMSCs) in vitro.We have investigated electrotaxis of bone marrow-derived MSCs using pulsed electric field (PEF) in range 16-80 mV/mm and frequency 130 Hz and 240 Hz. The PEF-related dynamics of the cell surface glycosylation was evaluated using six plant lectins.PEF at physiological levels (10mV/mm; 130 Hz) did not influence cellular motility in vitro, what may correspond to the maintenance of the transplanted cells at the lesion site in vivo. Increase of the PEF intensity and frequency above physiological levels resulted in the increase in the cellular migration rate in vitro. PEF elevated above physiological intensity and frequency (40-80 mV/mm; 240 Hz), but not at physiological levels, resulted in changes of the cell surface glycosylation.We find the described approach as convenient for investigations and for the in vitro modeling of the cellular systems intended for the regenerative cell transplantations in vivo. Probing cell surface glycomes may provide valuable biomarkers to assess competence of transplanted cells.

Phenotypic analysis of cell surface markers and gene expression of human mesenchymal stem cells and chondrocytes during monolayer expansion

Biorheology ◽  
2008 ◽  
Vol 45 (3-4) ◽  
pp. 513-526 ◽  
Author(s):  
Christel Cournil-Henrionnet ◽  
Céline Huselstein ◽  
Yun Wang ◽  
Laurent Galois ◽  
Didier Mainard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Human Mesenchymal Stem Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Phenotypic Analysis ◽  
Monolayer Expansion

Profiling and semiquantitative analysis of the cell surface proteome in human mesenchymal stem cells

2013 ◽  
Vol 405 (16) ◽  
pp. 5501-5517 ◽  
Author(s):  
Sang Kwang Lee ◽  
Jae Ho Kim ◽  
Sung-Soo Kim ◽  
Taewook Kang ◽  
Nam Hyun Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Human Mesenchymal Stem Cells ◽  
Semiquantitative Analysis ◽  
Surface Proteome ◽  
Cell Surface Proteome
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