scholarly journals Dinámica estacional de micorrizas arbusculares y hongos septados endofíticos oscuros en asociación con raíces de Solanum elaeagnifolium Cav.

2021 ◽  
Vol 99 (2) ◽  
pp. 291-304
Author(s):  
Pedro Osuna Avila ◽  
Juan Pedro Flores Margez ◽  
Baltazar Corral Díaz

Antecedentes: El trompillo es asociado por hongos endofíticos septados (HOS) y micorrizas arbusculares (MA) en ecosistemas tropicales, pero no se ha estudiado su colonización en cada etapa fenológica durante las estaciones del año en una zona urbana árida. Preguntas: ¿Cuáles estructuras fúngicas ocurren simultáneamente y están relacionadas con cada etapa de crecimiento del trompillo? Especies de estudio: Solanum elaeagnifolium Cav. Sitio de estudio y fecha: Ciudad Juárez, Chihuahua, México, 2014. Método: Las raíces se trataron con una tinción dual de azul de tripano y sudan IV para distinguir microscópicamente las estructuras de la colonización de ambos hongos en el ciclo de vida del trompillo en las cuatro estaciones del año. Se evaluó el promedio de sus estructuras fúngicas y su colonización total por milímetro de longitud de raíz. Resultados: La colonización simultánea de los hongos fue en todas las estaciones cuya diversidad morfológica estuvo relacionada con la fenología del hospedero. El promedio de la colonización de sus estructuras de ambos hongos (HOS: 64.2 ± 7.2 y HMA: 31.5 ± 3.2 por mm de longitud de raíz) representaron la mayor significancia de asociación de las raíces en primavera. Las hifas hialinas como las estructuras activas de los HOS y los arbúsculos de los HMA fueron consistentes en las estaciones del año. Conclusiones: Este es el primer reporte en mostrar la asociación micorrízica tipo ‘Arum’ y describir la co-ocurrencia de los HMA y los HOS en relación con la fenología del trompillo por estación del año en una zona urbana del Desierto Chihuahuense.

EPPO Bulletin ◽  
2007 ◽  
Vol 37 (1) ◽  
pp. 137-144 ◽  
Author(s):  
A. Ameur ◽  
Y. Baye ◽  
M. Bouhache ◽  
A. Taleb

EPPO Bulletin ◽  
2007 ◽  
Vol 37 (1) ◽  
pp. 125-128 ◽  
Author(s):  
S. Boukhris-Bouhachem ◽  
M. Hullé ◽  
J. Rouzé-Jouan ◽  
L. Glais ◽  
C. Kerlan

1938 ◽  
Vol s2-80 (318) ◽  
pp. 293-319
Author(s):  
MARGARET I. DANIELS

A. Three gregarine species are found to inhabit the mid-gut of the mealworm larvae used: Gregarina cuneata Stein, Gregarina polymorpha Hamm, and Gregarina steini Berndt. The often described Steinina ovalis is probably seldom or never found. They live only in the mid-gut of larvae. They are never found in pupal or adult forms. Gregarines have been seen moving when in a stratified condition. B. The gregarine cytoplasm has five important inclusions, each having a characteristic position in a centrifuged animal (Text-fig. 2). 1. Paraglycogen.--This gives a dark brown colour with iodine, a pinkish general colour with the acid fuchsin of the Feulgen technique, and often a red colour with Bauer's reaction. It occupies the centrifugal pole of the centrifuged cell and is in the form of disc-like granules of varying size. 2. In young centrifuged Gregarina steini chromidial granules are seen in the paraglycogen area, and have, therefore, approximately the same specific gravity. They arise by karyosomic budding with the subsequent extrusion of these buds into the cytoplasm. They stain with iron alum haematoxylin, like the karyosome, and both give a negative result with the Feulgen test for thymonucleic acid. They probably correspond to Joyet-Lavergne's ‘albuminoid reserves’, but do not have the mitochondrial‘cap’ he describes. 3. Mitochondria.--These are usually granular, but sometimes rod-like. They are seen between the ‘alveoli’ formed by the paraglycogen granules. They lie distally to the paraglycogen in a centrifuged parasite; they stain by the iron alum haematoxylin long method, after Benoit, Champy, or Altmann fixation, also with Altmann's fuchsin picric acid stain and the Bensley Cowdry modification of it. 4. The Nucleus is karyosomic, and the karyosome is moved to the centrifugal pole by pressure as is the nucleolus of metazoan cells. The nucleus shows budding of the karyosome. There is plasmatic as well as chromatic material in the karyosome, as shown by centrifuging. The nucleus gives a negative result with Feulgen's nuclear reaction, but chromatin may exist in a very dispersed condition. 5. Golgi Material.--This lies at the centripetal end of the nucleus. It is best shown by Weigl fixation. The large and regular Golgi elements are slightly heavier than the granular Golgi material, which may be compared with that of young oocytes. 6. Fatty Material lies at the extreme centripetal pole of the cell, in globules of varying size. It becomes brown or black after treatment with osmium tetroxide, and vivid cherry red with Sudan IV. It gives a negative result with the Schultz reaction for cholestrol. C. Large globules are seen in the protomerite of Gregarina steini , eosinophile, sometimes fuchsinophile, and also staining with methylene blue. These move towards the centrifugal pole. Methylene blue preparations show blue granules among the paraglycogen granules in the centrifuged animal. They are remarkably resistant to dilute sulphuric acid. They are possibly allied to volutin or chromidia. Tests for the presence of Vitamin C yielded negative results. Only the inclusions of the gregarines in the gut lumen were studied, and the complete life-cycles of the species were not followed out.


Author(s):  
Aidé Aracely Maldonado-Macías ◽  
Margarita Ortiz Solís ◽  
Oziely Daniela Armenta Hernández ◽  
Karla Janeth Hernández Luna ◽  
Jorge Luis García Alcaraz

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