scholarly journals Development of SSR markers and their application to genetic diversity analysis of Curcuma alismatifolia varieties

2020 ◽  
Vol 99 (1) ◽  
pp. 124-131
Author(s):  
Lihui Mao ◽  
Jianxin Liu ◽  
Huaqiao Ding ◽  
Qingcheng Zou ◽  
Danqing Tian
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Polyacrylamide Gel Electrophoresis ◽  
Diversity Analysis ◽  
Cut Flowers ◽  
Sequencing Technology ◽  
Genetic Diversity Analysis ◽  
Single Cell Sequencing ◽  
The Past ◽  
Maximum Proportion

Background: Curcuma alismatifolia is an ornamental cultivar with several varieties introduced into China from Thailand within the past fifteen years. Curcuma alismatifolia is widely used as cut flowers and potted flowers and is also used in flower beds. Questions and/or Hypotheses: However, limited genetic and genomic information is available for this species, which has impeded studies on the enhancement of its ornamental value and stress resistance. Studied species Curcuma alismatifolia Study site and dates: Zhejiang, 2018   Methods: single-cell sequencing technology of PCR and polyacrylamide gel electrophoresis   Results: (A/T)n accounted for 43.6 % of the SSRs, the maximum proportion, and mononucleotide and trinucleotide repeats were the two most abundant repeat types. A total of 3,637 primer pairs flanking SSR sequences were successfully designed, and 70 sets of primers were randomly selected for validation in 10 varieties. Forty-one (59 %) of the 70 primer pairs successfully amplified alleles, of which 35 were identified as polymorphic markers and used to assess the level of genetic diversity and genetic relationships among the 10 varieties. The genetic diversity analysis showed that the number of alleles (Na) at each locus ranged from 2 to 8, with an average of 3.97, and that PIC had a mean of 0.524 and ranged from 0.095 to 0.795. Conclusions: The genetic distance between 10 varieties varied from 0.30 to 0.96, and the dendrogram clustered all varieties into three groups.

scholarly journals Molecular identification and genetic relationships among coffee species (Coffea L.) inferred from ISSR and SRAP marker analyses

2011 ◽  
Vol 63 (3) ◽  
pp. 667-679 ◽  
Author(s):  
Kumar Mishra ◽  
Sandhyarani Nishani ◽  
J Jayarama
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Resolving Power ◽  
Diversity Analysis ◽  
Srap Marker ◽  
Genetic Diversity Analysis ◽  
Species Specific ◽  
Coffee Species ◽  
Issr Primers

The identification and genetic relationships of 23 coffee species and one coffee-related species Canthium diccocum were studied using ISSR and SRAP markers. The average polymorphism information content of SRAP primers (0.81) was lower than ISSR primers (0.86), whereas the average resolving power of the SRAP primers (9.74) is higher than the ISSR primers (8.64). The genetic similarity among the species ranged from 0.30 to 0.89 using ISSR and 0.11 to 0.90 using SRAP marker systems. Based on marker analysis, all twenty three coffee species were clustered into two major groups. Both the markers amplified species-specific fragments and are useful in genetic diversity analysis of coffee.

scholarly journals PENGGUNAAN PENANDA MOLEKULER RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) UNTUK ANALISIS KERAGAMAN GENETIK KELAPA WEST AFRICAN TALL (WAT)

EUGENIA ◽  
2005 ◽  
Vol 11 (3) ◽  
Author(s):  
Edy F. Lengkong ◽  
S. D. Runtunuwu
Keyword(s):  
Genetic Diversity ◽  
Molecular Marker ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
West African ◽  
Polymorphic Band ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Total Dna ◽  
Genetic Combination

ABSTRACT Lengkong, E.F. and S.D. Runtunuwu. 2005. Use of Molecular Marker Random Amplified Polymorphic DNA (RAPD) to Genetic Diversity Analysis of West African Tall (WAT) Coconut. Eugenia 11 (3):210-217.   Information on genetic diversity of crop gemplasm has several important implications for plant breeder. Among others is to help the breeders to decide what sources to cross so as to making new genetic combination. The use of molecular marker to genetic diversity analysis on DNA level is usefull, because it provides an opportunity to more precisely measure genetic relationships as well is not affected by the environment. The objective of this research was to analyze genetic diversity of West African Tall (WAT) using molecular marker RAPD. Five arbitrary 10-mer primers were used to amplify total DNA genom, and to generate 44 band DNA with 26 band or 59 % were polymorphic band. It was revealed that genetic diversity within population of WAT coconut was 14 %. Based on cluster analysis, at the genetic similarity 85% or genetic diversity 15 % the population was separated on three clusters. This research concluded that the population of WAT coconut grown from open pollinated seeds has different genotype one each others. Keywords: West African Tall (WAT), Coconut, RAPD

scholarly journals Development and characterization of STMS molecular markers and genetic diversity analysis of mungbean [Vigna radiata (L.) Wilczek]

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
Sujan S. Bimal ◽  
S. P. Chavan ◽  
A. B. Gaikwad ◽  
K. V. Bhat
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Microsatellite Loci ◽  
Mung Bean ◽  
Genomic Library ◽  
Genetic Relationships ◽  
Genomic Research ◽  
Group Method ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

Mung bean is an important crop in Asia because of its high protein content and other economic uses. However, because of the unavailability of polymorphic DNA markers, genomic research of mung bean is lacking. In this study, we developed and characterised simple sequence repeat (SSR) molecular markers by screening SSR-enriched partial genomic libraries with SSR probes and used them to analyse the genetic diversity of mung bean. Thus, we isolated, cloned, sequenced a genomic library that contained microsatellite loci from the mung bean variety ‘MCV-1’. The polymorphisms of microsatellite loci were evaluated using the unweighted pair group method of arithmetic means, and MDS cluster analysis showed genetic relationships in a panel of 96 mung bean core collection genotypes. Genetic diversity analysis results showed contrasted levels of variability within cultivated and wild accessions. A total of 98 alleles were detected using 19 polymorphic markers, with an average of 4.9 alleles per locus, whereas observed heterozygosity ranged from 0.1 to 0.5, with a mean of 0.42 per locus. The number of alleles and the high level of polymorphism make these new markers useful for gene tagging, diversity analyses and marker assisted selection in mung bean.

Sign in / Sign up

Export Citation Format

Share Document