scholarly journals Lipid Composition of Sheffersomyces stipitis M12 Strain Grown on Glycerol as a Carbon Source

2020 ◽  
Vol 58 (2) ◽  
pp. 203-213
Author(s):  
Stela Križanović ◽  
Damir Stanzer ◽  
Gordana Čanadi Jurešić ◽  
Elizabeta Kralj ◽  
Karla Hanousek-Čiča ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Carbon Source ◽  
Lipid Composition ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Wild Strain ◽  
Yeast Cells ◽  
Sterol Esters ◽  
Ergosterol Synthesis

Research background. In this study the content and composition of lipids in ergosterol-reduced Sheffersomyces stipitis M12 strain grown on glycerol as a carbon source is determined. Blocking the ergosterol synthesis route in yeast cells is a recently proposed method for increasing S-adenosyl-L-methionine (SAM) production. Experimental approach. The batch cultivation of M12 yeast was carried out under aerobic conditions in a laboratory bioreactor with glycerol as carbon source, and with pulsed addition of methionine. Glycerol and SAM content were monitored by high-performance liquid chromatography, while fatty acid composition of different lipid classes, separated by solid phase extraction, was determined by gas chromatography. Results and conclusion. Despite the reduced amount of ergosterol in yeast cells, thanks to the reorganized lipid metabolism, M12 strain achieved high biomass yield and SAM production. Neutral lipids prevailed (making more than 75 % of total lipids), but their content and composition differed significantly in the two tested types of yeast. Unsaturated and C18 fatty acids prevailed in both the M12 strain and wild type. In all fractions except free fatty acids, the index of unsaturation in M12 strain was lower than in the wild strain. Our tested strain adjusts itself by changing the content of lipids (mainly phospholipids, sterols and sterol esters), and with desaturation adjustments, to maintain proper functioning and fulfil increased energy needs.Novelty and scientific contribution. Reorganization of S. stipitis lipid composition caused by blocking the metabolic pathway of ergosterol synthesis was presented. A simple scheme of actual lipid metabolism during active SAM production in S. stipitis, grown on glycerol was constructed and shown. This fundamental knowledge of lipid metabolic pathways will be a helpful tool in improving S. stipitis as an expression host and a model organism, opening new perspectives for its applied research.

Production of lipids and lipase activity during growth of Mucor hiemalis

1982 ◽  
Vol 28 (6) ◽  
pp. 618-622 ◽  
Author(s):  
A. Qayum Mirza ◽  
M. Waheed Akhtar ◽  
M. Nadeem Nawazish ◽  
M. I. D. Chughtai
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Lipid Content ◽  
Growth Phase ◽  
Lipase Activity ◽  
Polar Lipid ◽  
Neutral Lipids ◽  
Cell Lysis ◽  
Mucor Hiemalis ◽  
Sterol Esters

Production of lipids and lipase activity by a strain of Mucor hiemalis known to have potential for high lipase activity was studied in a medium containing glucose as the carbon source. Most of the glucose was utilized by the end of the growth phase when the mycelium showed maximal lipase activity (17 U/g dry mycelium) as well as maximal lipid content (13 mg/g dr*** mycelium). Maximal broth lipase activity (76 U/100mL) was observed after cell lysis had begun. The fractions identified in the mycelial lipid extracts were sterol esters, triglycerides, fatty acids, diglycerides, sterols, monoglycerides, phosphatidylethanoamine, phosphatidylcholine, phosphatidylserine, and an unknown polar lipid. The proportion between the neutral and the polar lipids was only 0.06 at 44 h, increasing gradually to 1.51 at 164 h of fermentation. A relationship between the production of lipase and the neutral lipids appears to exist.

Lipid profiles of Aspergillus niger and its unsaturated fatty acid auxotroph, UFA2

1985 ◽  
Vol 31 (4) ◽  
pp. 352-355 ◽  
Author(s):  
Panchanon Chattopadhyay ◽  
Santu Kumar Banerjee ◽  
Kalyani Sen ◽  
Parul Chakrabarti
Keyword(s):  
Fatty Acid ◽  
Aspergillus Niger ◽  
Unsaturated Fatty Acid ◽  
Lipid Composition ◽  
Unsaturated Fatty Acids ◽  
Neutral Lipids ◽  
Wild Strain ◽  
Wild Type ◽  
Sterol Esters ◽  
Fatty Acid Auxotroph

A comparative study of the mycelial lipid composition of a wild strain (V35) and one unsaturated fatty acid auxotroph (UFA2) of Aspergillus niger has been performed. The lipid composition of both strains are qualitatively the same but quantitatively different. All the strains contain the following phospholipids: cardiolipin, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylcholine, and phosphatidylserine; and triglycerides, diglycerides, mono-glycerides, ergosterol, and sterol esters as the neutral lipids; mono- and di-galactosyl diglyceride as the major glycolipids along with small amounts of the corresponding mannose analogs. Phosphatidylethanolamine and phosphatidylcholine constitute the bulk of the phospholipids. The mutant (UFA2) contains a higher level of glycerides and lower levels of sterol (both free and esterified form), phospholipids, and glycolipids than the wild type. Aspergillus niger contains C16 to C18 saturated and unsaturated fatty acids. Small amounts of long-chain (C20 to C24) and short-chain (C10 to C14) saturated and unsaturated acids are also present. Linoleic, oleic, and palmitic are the major acids, stearic and linolenic acids being minor ones. UFA2 grows only in the presence of unsaturated fatty acid (C16 or C18) and accumulates a higher concentration of supplemented acid which influences its fatty acid profile.

scholarly journals Identification and Quantification of Fatty Acids in T. viridissima, C. biguttulus, and C. brunneus by GC-MS

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Solid Phase Extraction ◽  
Free Fatty Acids ◽  
Dry Matter ◽  
Solid Phase ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Phase Extraction ◽  
Chorthippus Biguttulus

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.

scholarly journals Fatty Acid Components of Ovine Erythrocyte Lipids

1965 ◽  
Vol 18 (2) ◽  
pp. 445 ◽  
Author(s):  
JM Connellan ◽  
CJ Masters
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Structure Function ◽  
Lipid Composition ◽  
Mammalian Erythrocyte ◽  
Mammalian Tissues ◽  
Membrane Type ◽  
And Function ◽  
Erythrocyte Lipids

As part of a study of comparative lipid metabolism, the distribution of fatty acids has been investigated in a number of mammalian tissues (Horgan and Masters 1963; Masters 1964a, 1964b, 1964c; Connellan and Masters 1965), a major aim of these studies being to facilitate correlation between lipid composition and function. In this context, it is widely recognized that membranes playa fundamental role in cellular metabolism, and that lipid is an essential component of these biomembranes (Stein and Danielli 1956). The study of structure-function relationships in this situation, however, has been hindered by the difficulty of isolating specific membranes without contamination by other lipids. The mature mammalian erythrocyte possesses advantageous characteristics for this type of investigation because of the lack of sub-cellular particles, and the resultant presence of only a single membrane type (Kogl et al. 1960). As an extension of previous investigations, then, the fatty acid composi-tion of ovine erythrocytes has been determined.

scholarly journals Lipid composition of raw and cooked Rapana venosa from the Black Sea

2018 ◽  
Vol 29 (2) ◽  
pp. 49-55 ◽  
Author(s):  
Albena Merdzhanova ◽  
Veselina Panayotova ◽  
Diana A. Dobreva ◽  
Rostitsa Stancheva ◽  
Katya Peycheva
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Lipid Composition ◽  
Neutral Lipids ◽  
Fatty Acid Distribution ◽  
Lipid Classes ◽  
The Black Sea ◽  
Limited Information ◽  
Rapana Venosa ◽  
Fat Soluble Vitamins

Abstract Rapana venosa is an edible mollusc with nutritional and economic importance. There is limited information about its lipid composition. The aim of the present study is to provide information about lipid composition, fatty acid profiles, fat soluble vitamins and cholesterol content of raw and cooked Rapana venosa. Cooking did not affect the ratio of lipid classes, but fatty acids composition varied significantly. Considerable variations were observed in fatty acid distribution of total lipids and neutral lipids. Fatty acid groups of phospholipids remained unaffected by temperature treatment. The most abundant fatty acids in all lipid classes of raw and cooked specimens were palmitic acid (C16:0) and eicosapentaenoic acid (C20:5n-3). The sum of omega-3 polyunsaturated fatty acids (PUFA) was higher than omega-6 PUFA in all lipid fractions. The results of the present study showed that cooking process affected cholesterol, fat soluble vitamins and carotenoids content differently. Larger variations were observed for vitamin A, β-carotene and astaxanthin and to lesser for vitamin E. Cholesterol and vitamin D3 were also affected by the thermal stress. The present study revealed that Rapana venosa meat could be a good source of high quality nutritional lipids, which are well preserved even after culinary treatment

Myocardial lipid metabolism in cardiac hyper- and hypo-function. Studies on triiodothyronine-treated and transplanted rat hearts

1977 ◽  
Vol 55 (6) ◽  
pp. 1311-1319 ◽  
Author(s):  
S. C. Vasdev ◽  
B. Korecky ◽  
R. B. Rastogi ◽  
R. L. Singhal ◽  
K. J. Kako
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Erucic Acid ◽  
Experimental Model ◽  
Lipid Composition ◽  
Mechanical Performance ◽  
Phospholipid Composition ◽  
Adult Rats ◽  
Rat Hearts

Lipid composition of the myocardium and in vitro lipid metabolism were studied in hearts from young rats after 30 days of treatment with triiodothyronine (100 μg/kg per day) and in heterotopically isotransplanted hearts of inbred adult rats 6 days after surgery. The former served as an experimental model of cardiac hyperfunction, while the latter, empty beating hearts, served as a model of cardiac hypofunction. In hearts from hyperthyroid animals the concentration of phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and the incorporation of 14C-labelled palmitic and erucic acid into these phospholipids were increased significantly as compared with controls. In contrast, the triglyceride concentration and the incorporation of palmitate into triglyceride were significantly decreased. In transplanted hearts, the phospholipid concentration and the incorporation of 14C-labelled fatty acids into phospholipids were significantly decreased as compared with the hearts of the inbred host rats of the same age. The results indicate that the mechanical performance of the heart affects the phospholipid composition, which maybe a reflection of increased or decreased proliferation of subcellular membranes in sustained cardiac hyper- or hypo-function.

Acetate assimilation by the fission yeast, Schizosaccharomyces pombe

1989 ◽  
Vol 67 (8) ◽  
pp. 464-467 ◽  
Author(s):  
C. S. Tsai ◽  
Ken P. Mitton ◽  
Byron F. Johnson
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Neutral Lipids ◽  
Glyoxylate Cycle ◽  
Yeast Cells ◽  
Peptide Structure ◽  
Synthetase Activity ◽  
Sterol Esters ◽  
Acetate Utilization ◽  
Structure Of Proteins

The fission yeast Schizosaccharomyces pombe utilizes acetate at subinhibitory concentrations in the presence of D-glucose. The nonionized form of acetate is preferentially utilized, oxidized to 14CO2, and assimilated into lipids and proteins. Acetyl CoA synthetase activity greatly increases in the yeast cells grown in media containing acetate. However, glyoxylate cycle enzymes are not detectable in Schizosaccharomyces pombe. [1-14C] Acetate is incorporated into stereols, sterol esters, neutral lipids, and phospholipids. Assimilation of [1-14C]acetate into the peptide structure of proteins was confirmed by a proteolytic digestion experiment.Key words: acetate utilization, fission yeast, Schizosaccharomyces pombe.

scholarly journals STUDY OF THE LIPID COMPOSITION OF SOME PLANTS GROWING IN GEORGIA

2019 ◽  
pp. 24-25
Author(s):  
B. Kikalishvili ◽  
Ts. Sulakvelidze ◽  
M. Malania ◽  
D. Turabelidze
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitis Vinifera ◽  
Lipid Composition ◽  
High Performance ◽  
Neutral Lipids ◽  
Physical Chemical ◽  
Neutral And Polar Lipids

Sums of neutral and polar lipids were extracted from the fruits of Vitis vinifera and pericarpium of Physalis alkekengi L.; their main classes and physical-chemical constants of some neutral lipids were determined. The saturated, unsaturated and polyunsaturated fatty acids were identified by using High Performance Liquid Chromatography. The phospholipids- lysophosphatidylcholine, phosphatidylinosite, phosphatidylcholine, phosphatidylethanolamine and N-acyl phosphatidylethanolamine were identified in them. The content of carotenoids is up 2,5 mg% and 65,5mg% in Vitis vinifera and Physalis alkekengi L. respectively. Amino acids were detected in studied species. The oils from both plants are characterized with anti-inflammatory, hepatoprotective, choleretic and immunotropic activities, they can be used in medicine for curative and preventive purposes.

scholarly journals Oil-lipids, carotenoids and fatty acids simultaneous production by Rhodotorula mucilaginosa CCT3892 using sugarcane molasses as carbon source

2020 ◽  
Vol 23 ◽  
Author(s):  
Willyan Araújo da Costa ◽  
Carlos Eduardo de Araújo Padilha ◽  
Sérgio Dantas de Oliveira Júnior ◽  
Flávio Luiz Honorato da Silva ◽  
Josevan Silva ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Yeast Cells ◽  
Food Supplementation ◽  
Sugarcane Molasses ◽  
Rhodotorula Mucilaginosa ◽  
Total Lipids ◽  
Simultaneous Production ◽  
Ability To Act ◽  
Wide Range

Abstract One of the most important classes of nutritional biomolecules is the oleaginous compounds group, which specially includes the oil-lipids, the carotenoids and the fatty acids. These biocompounds present a wide range of industrial applications because their ability to act as an energy source, antioxidants and metabolic agents for the human body. Therefore, the food industry, mainly focusing on food supplementation, is always searching for new sources of them. In this context, the present study evaluated the total lipids, carotenoids and fatty acids simultaneous production by the Rhodotorula mucilaginosa CCT3892 yeast, using residual sugarcane molasses as carbon source. The results obtained demonstrated that the cultivation of yeast in molasses medium (MC) produced the same content of total lipids and carotenoids (16.50% ± 0.68% and 0.053 ± 0.001 mg g-1, respectively) as the obtained from a synthetic medium (SC) (15.36% ± 1.36% and 0.051 ± 0.001 mg g-1 0.005). Concerning the fatty acids biosynthesis, the MC cultivation generated the most interesting profile once it presented a greater content of oleic acid (74.05%), an unsaturated compound with high nutritional value. The cultivation carried out with the molasses and yeast extract supplementation (MYEC) did not provide an improvement in microbial oil production, what indicated that in this condition there was a predominance of others sorts of substrate metabolization by the yeast cells, as confirmed by the microbial kinetics study.

Effects of unsaturated fatty acid deprivation on neutral lipid synthesis in Saccharomyces cerevisiae

1982 ◽  
Vol 152 (2) ◽  
pp. 747-756
Author(s):  
T M Buttke ◽  
A L Pyle
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Cell Growth ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Lipid Synthesis ◽  
Yeast Cells

The effects of unsaturated fatty acid deprivation on lipid synthesis in Saccharomyces cerevisiae strain GL7 were determined by following the incorporation of [14C]acetate. Compared to yeast cells grown with oleic acid, unsaturated fatty acid-deprived cells contained 200 times as much 14C label in squalene, with correspondingly less label in 2,3-oxidosqualene and 2,3;22,23-dioxidosqualene. Cells deprived of either methionine or cholesterol did not accumulate squalene, demonstrating that the effect of unsaturated fatty acid starvation on squalene oxidation was not due to an inhibition of cell growth. Cells deprived of olefinic supplements displayed additional changes in lipid metabolism: (i) an increase in 14C-labeled diacylglycerides, (ii) a decrease in 14C-labeled triacylglycerides, and (iii) increased levels of 14C-labeled decanoic and dodecanoic fatty acids. The changes in squalene oxidation and acylglyceride metabolism in unsaturated fatty acid-deprived cells were readily reversed by adding oleic acid. Pulse-chase studies demonstrated that the [14C]squalene and 14C-labeled diacylglycerides which accumulated during starvation were further metabolized when cells were resupplemented with oleic acid. These results demonstrate that unsaturated fatty acids are essential for normal lipid metabolism in yeasts.

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