scholarly journals ALP LEVELS OF MURINE PRE-OSTEOBLASTIC CELLS ON ANODIZED AND ANNEALED TITANIUM SURFACES

2017 ◽  
Vol 0 (0) ◽  
Author(s):  
Sinem Yeniyol ◽  
John Lawrence Ricci
Keyword(s):  
Osteoblastic Cells ◽  
Titanium Surfaces

scholarly journals Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Carlos Nelson Elias ◽  
Patricia Abdo Gravina ◽  
Costa e Silva Filho ◽  
Pedro Augusto de Paula Nascente
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Osteoblastic Cells ◽  
Acid Etching ◽  
Implant Surface ◽  
Force Microscopy ◽  
Dental Implant Surface ◽  
Before And After ◽  
Titanium Surfaces

Statement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength.Objective. In this work, the effects of dental implant surface treatment and fibronectin adsorption on the adhesion of osteoblasts were analyzed.Materials and Methods. Two titanium dental implants (Porous-acid etching and PorousNano-acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy, and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine their effects on the interaction with osteoblastic cells.Results. The evaluation techniques used showed that the Porous and PorousNano implants have similar microstructural characteristics. Spectrophotometry demonstrated similar levels of fibronectin adsorption on both surfaces (80%). The association indexes of osteoblastic cells in FN-treated samples were significantly higher than those in samples without FN. The radioactivity values associated with the same samples, expressed as counts per minute (cpm), suggested that FN incorporation is an important determinant of thein vitrocytocompatibility of the surfaces.Conclusion. The preparation of bioactive titanium surfaces via fluoride and FN retention proved to be a useful treatment to optimize and to accelerate the osseointegration process for dental implants.

Transcriptional profile of osteoblastic cells cultured on titanium surfaces modified by oxidative nanopatterning

2014 ◽  
Author(s):  
Maidy Ferreira ◽  
Fabio Variola ◽  
Antonio Nanci ◽  
Janaina Dernowsek ◽  
Geraldo Passos ◽  
...  
Keyword(s):  
Transcriptional Profile ◽  
Osteoblastic Cells ◽  
Titanium Surfaces ◽  
Cells Cultured

scholarly journals Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Maidy Rehder Wimmers Ferreira ◽  
Roger Rodrigo Fernandes ◽  
Amanda Freire Assis ◽  
Janaína A. Dernowsek ◽  
Geraldo A. Passos ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Control Cell ◽  
Titanium Implants ◽  
Osteoblastic Cells ◽  
Surface Features ◽  
Cellular Events ◽  
Tissue Interactions ◽  
Biochemical Assays ◽  
Titanium Surfaces ◽  
Dental Applications

Titanium implants have been extensively used in orthopedic and dental applications. It is well known that micro- and nanoscale surface features of biomaterials affect cellular events that control implant-host tissue interactions. To improve our understanding of how multiscale surface features affect cell behavior, we used microarrays to evaluate the transcriptional profile of osteoblastic cells from human alveolar bone cultured on engineered titanium surfaces, exhibiting the following topographies: nanotexture (N), nano+submicrotexture (NS), and rough microtexture (MR), obtained by modulating experimental parameters (temperature and solution composition) of a simple yet efficient chemical treatment with a H2SO4/H2O2solution. Biochemical assays showed that cell culture proliferation augmented after 10 days, and cell viability increased gradually over 14 days. Among the treated surfaces, we observed an increase of alkaline phosphatase activity as a function of the surface texture, with higher activity shown by cells adhering onto nanotextured surfaces. Nevertheless, the rough microtexture group showed higher amounts of calcium than nanotextured group. Microarray data showed differential expression of 716 mRNAs and 32 microRNAs with functions associated with osteogenesis. Results suggest that oxidative nanopatterning of titanium surfaces induces changes in the metabolism of osteoblastic cells and contribute to the explanation of the mechanisms that control cell responses to micro- and nanoengineered surfaces.

Adhesion pattern and growth of primary human osteoblastic cells on five commercially available titanium surfaces

2010 ◽  
Vol 21 (7) ◽  
pp. 756-765 ◽  
Author(s):  
Giovanni Passeri ◽  
Antonio Cacchioli ◽  
Francesca Ravanetti ◽  
Carlo Galli ◽  
Erida Elezi ◽  
...  
Keyword(s):  
Osteoblastic Cells ◽  
Titanium Surfaces ◽  
Human Osteoblastic Cells ◽  
Adhesion Pattern

In vitro assays for adhesion and migration of osteoblastic cells (Saos-2) on titanium surfaces

2006 ◽  
Vol 324 (3) ◽  
pp. 369-375 ◽  
Author(s):  
Chun-yu Li ◽  
Shuang-yan Gao ◽  
Takehiro Terashita ◽  
Tetsuya Shimokawa ◽  
Haruyuki Kawahara ◽  
...  
Keyword(s):  
Osteoblastic Cells ◽  
In Vitro Assays ◽  
Titanium Surfaces ◽  
And Migration

Effect of plasma‐nitrided titanium surfaces on the differentiation of pre‐osteoblastic cells

2019 ◽  
Vol 43 (8) ◽  
pp. 764-772 ◽  
Author(s):  
Carlos Eduardo B. Moura ◽  
Moacir F. Queiroz Neto ◽  
Janine Karla F. S. Braz ◽  
Michelle de Medeiros Aires ◽  
Naisandra B. Silva Farias ◽  
...  
Keyword(s):  
Osteoblastic Cells ◽  
Titanium Surfaces ◽  
Nitrided Titanium

Attachment of oral bacteria to titanium surfaces: An SEM study

1994 ◽  
Vol 52 ◽  
pp. 468-469
Author(s):  
J. E. Laffoon ◽  
R. L. Anderson ◽  
J. C. Keller ◽  
C. D. Wu-Yuan
Keyword(s):  
Technical Problem ◽  
Titanium Implant ◽  
Oral Bacteria ◽  
Bacterial Cells ◽  
Thin Sections ◽  
Surface Ultrastructure ◽  
Sem Study ◽  
Key Factor ◽  
Titanium Surfaces

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).

ROLE OF rhBMP-2 AND rhBMP-7 IN THE METABOLISM AND DIFFERENTIATION OF OSTEOBLAST-LIKE CELLS CULTURED ON CHEMICALLY MODIFIED TITANIUM SURFACES

2012 ◽  
pp. 141208072802005
Author(s):  
Fabiano Ribeiro Cirano ◽  
ADRIANE TOGASHI ◽  
MARCIA MARQUES ◽  
FRANCISCO PUSTIGLIONI ◽  
LUIZ LIMA
Keyword(s):  
Chemically Modified ◽  
Titanium Surfaces ◽  
Cells Cultured
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