Effects of the antimalarial drugs ferroquine and artesunate on Plasmodium yoelii yoelii gametocytegenesis and vectorial transmission

2011 ◽  
Vol 21 (3) ◽  
pp. 133-142
Author(s):  
Kamla Mustfa ◽  
Irène Landau ◽  
Alain-Gabriel Chabaud ◽  
Jean-Marc Chavatte ◽  
Jacques Chandenier ◽  
...  
Keyword(s):  
Plasmodium Yoelii ◽  
Antimalarial Drugs ◽  
Plasmodium Yoelii Yoelii

Variable expression of virulence in the rodent malaria parasitePlasmodium yoelii yoelii

Parasitology ◽  
1980 ◽  
Vol 81 (1) ◽  
pp. 211-219 ◽  
Author(s):  
G. Knowles ◽  
D. Walliker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Marked Decrease ◽  
Plasmodium Yoelii ◽  
Intermediate Level ◽  
Rodent Malaria ◽  
Variable Expression ◽  
Plasmodium Yoelii Yoelii

SUMMARYThe expression of the virulence character in the virulent line (YM) ofPlasmodium yoelii yoeliiwas investigated. The level of virulence was measured by counting the parasitaemia of the mature red blood cells. Several sub-clones were isolated from the virulent line YM and each was tested for its level of virulence. Out of 10 sub-clones 1 showed a marked decrease in virulence. However, transmission of this sub-clone through mosquitoes fully restored its virulence. A clone isolated from the progeny of a cross between mild and virulent parents had an intermediate level of virulence. A sub-clone isolated from this intermediate virulent line exhibited greatly reduced virulence. Mosquito transmission of this sub-clone also restored its virulence to a level comparable with the virulent line YM.

Evasion of macrophage microbicidal mechanisms by mature sporozoites of Plasmodium yoelii yoelii

Parasitology ◽  
1986 ◽  
Vol 93 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Judith E. Smith ◽  
James Alexander
Keyword(s):  
Salivary Gland ◽  
Respiratory Burst ◽  
Peritoneal Macrophages ◽  
Plasmodium Yoelii ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Oxidative Response ◽  
Plasmodium Yoelii Yoelii ◽  
Relationship Of ◽  
The Relationship

SUMMARYSporozoites of Plasmodium yoelii yoelii were incubated for 40 min with BALB/c peritoneal macrophages in the presence of nitro-blue tetrazolium (NBT). While immature oocyst sporozoites triggered the macrophage respiratory burst, as visualized microscopically by the localized reduction of NBT to insoluble formazan, 97·6% of mature salivary gland sporozoites did not induce such a response. The macrophage oxidative response was also induced by 82·7% of heat-inactivated and 95·7% of trypsin-treated salivary gland sporozoites. The relationship of these results to the infectivity and immunogenicity of malarial sporozoites is discussed.

scholarly journals In Vitro Activities of 25 Quinolones and Fluoroquinolones against Liver and Blood Stage Plasmodium spp

2003 ◽  
Vol 47 (8) ◽  
pp. 2636-2639 ◽  
Author(s):  
Nassira Mahmoudi ◽  
Liliane Ciceron ◽  
Jean-François Franetich ◽  
Khemais Farhati ◽  
Olivier Silvie ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Drug Concentration ◽  
Blood Cells ◽  
Inhibitory Effect ◽  
Plasmodium Yoelii ◽  
Prolonged Incubation ◽  
Plasmodium Yoelii Yoelii ◽  
Effective Drugs

ABSTRACT The in vitro activities of 25 quinolones and fluoroquinolones against erythrocytic stages of Plasmodium falciparum and against liver stages of Plasmodium yoelii yoelii and P. falciparum were studied. All compounds were inhibitory for chloroquine-sensitive and chloroquine-resistant P. falciparum grown in red blood cells. This inhibitory effect increased with prolonged incubation and according to the logarithm of the drug concentration. Grepafloxacin, trovafloxacin, and ciprofloxacin were the most effective drugs, with 50% inhibitory concentrations of <10 μg/ml against both strains. Only grepafloxacin, piromidic acid, and trovafloxacin had an inhibitory effect against hepatic stages of P. falciparum and P. yoelii yoelii; this effect combined reductions of the numbers and the sizes of schizonts in treated cultures. Thus, quinolones have a potential for treatment or prevention of malaria through their unique antiparasitic effect against erythrocytic and hepatic stages of Plasmodium.

scholarly journals Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs

Pharmaceutics ◽  
2018 ◽  
Vol 10 (4) ◽  
pp. 225 ◽  
Author(s):  
Elisabet Martí Coma-Cros ◽  
Arnau Biosca ◽  
Joana Marques ◽  
Laura Carol ◽  
Patricia Urbán ◽  
...  
Keyword(s):  
Antimalarial Activity ◽  
Plasmodium Yoelii ◽  
Antimalarial Drugs ◽  
Mass Spectrometry Analysis ◽  
Parasite Invasion ◽  
Spectrometry Analysis ◽  
Anopheles Atroparvus ◽  
Oral Formulations ◽  
Preferential Binding ◽  
Direct Administration

Current strategies for the mass administration of antimalarial drugs demand oral formulations to target the asexual Plasmodium stages in the peripheral bloodstream, whereas recommendations for future interventions stress the importance of also targeting the transmission stages of the parasite as it passes between humans and mosquitoes. Orally administered polyamidoamine (PAA) nanoparticles conjugated to chloroquine reached the blood circulation and cured Plasmodium yoelii-infected mice, slightly improving the activity of the free drug and inducing in the animals immunity against malaria. Liquid chromatography with tandem mass spectrometry analysis of affinity chromatography-purified PAA ligands suggested a high adhesiveness of PAAs to Plasmodium falciparum proteins, which might be the mechanism responsible for the preferential binding of PAAs to Plasmodium-infected erythrocytes vs. non-infected red blood cells. The weak antimalarial activity of some PAAs was found to operate through inhibition of parasite invasion, whereas the observed polymer intake by macrophages indicated a potential of PAAs for the treatment of certain coinfections such as Plasmodium and Leishmania. When fluorescein-labeled PAAs were fed to females of the malaria mosquito vectors Anopheles atroparvus and Anopheles gambiae, persistent fluorescence was observed in the midgut and in other insect’s tissues. These results present PAAs as a versatile platform for the encapsulation of orally administered antimalarial drugs and for direct administration of antimalarials to mosquitoes, targeting mosquito stages of Plasmodium.

scholarly journals P188 Study of protective antibody epitope in MSP-1 of Plasmodium yoelii yoelii

2001 ◽  
Vol 52 (Supplement) ◽  
pp. 124
Author(s):  
Tetsuro SUZUKI ◽  
Morikazu NISHIHIRA ◽  
Yasuhiro YASUTOMI
Keyword(s):  
Plasmodium Yoelii ◽  
Protective Antibody ◽  
Antibody Epitope ◽  
Plasmodium Yoelii Yoelii

scholarly journals Assessment and improvement of the Plasmodium yoelii yoelii genome annotation through comparative analysis

2008 ◽  
Vol 24 (13) ◽  
pp. i383-i389 ◽  
Author(s):  
Ashley Vaughan ◽  
Sum-Ying Chiu ◽  
Gowthaman Ramasamy ◽  
Ling Li ◽  
Malcolm J. Gardner ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Genome Annotation ◽  
Plasmodium Yoelii ◽  
Plasmodium Yoelii Yoelii

scholarly journals Gene encoding erythrocyte binding ligand linked to blood stage multiplication rate phenotype in Plasmodium yoelii yoelii

2009 ◽  
Vol 106 (17) ◽  
pp. 7161-7166 ◽  
Author(s):  
Sittiporn Pattaradilokrat ◽  
Richard L. Culleton ◽  
Sandra J. Cheesman ◽  
Richard Carter
Keyword(s):  
Linkage Group ◽  
Plasmodium Yoelii ◽  
Blood Stage ◽  
Multiplication Rate ◽  
Gene Encoding ◽  
Genome Wide ◽  
Erythrocyte Binding ◽  
Plasmodium Yoelii Yoelii ◽  
Rate Selection ◽  
Severity Of The Disease

Variation in the multiplication rate of blood stage malaria parasites is often positively correlated with the severity of the disease they cause. The rodent malaria parasite Plasmodium yoelii yoelii has strains with marked differences in multiplication rate and pathogenicity in the blood. We have used genetic analysis by linkage group selection (LGS) to identify genes that determine differences in multiplication rate. Genetic crosses were generated between genetically unrelated, fast- (17XYM) and slowly multiplying (33XC) clones of P. y. yoelii. The uncloned progenies of these crosses were placed under multiplication rate selection in blood infections in mice. The selected progenies were screened for reduction in intensity of quantitative genetic markers of the slowly multiplying parent. A small number of strongly selected markers formed a linkage group on P. y. yoelii chromosome 13. Of these, that most strongly selected marked the gene encoding the P. yoelii erythrocyte binding ligand (pyebl), which has been independently identified by Otsuki and colleagues [Otsuki H, et al. (2009) Proc Natl Acad Sci USA 106:10.1073/pnas.0811313106] as a major determinant of virulence in these parasites. In an analysis of a previous genetic cross in P. y. yoelii, pyebl alleles of fast- and slowly multiplying parents segregated with the fast and slow multiplication rate phenotype in the cloned recombinant progeny, implying the involvement of the pyebl locus in determining the multiplication rate. Our genome-wide LGS analysis also indicated effects of at least 1 other locus on multiplication rate, as did the findings of Otsuki and colleagues on virulence in P. y. yoelii.

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