Droplet digital PCR, a new approach to analyze fetal DNA from maternal blood: application to the determination of fetal RHD genotype

2016 ◽  
Vol 74 (3) ◽  
pp. 269-277
Author(s):  
Lucie Orhant ◽  
Sophie Rondeau ◽  
Aurélie Vasson ◽  
Olivia Anselem ◽  
François Goffinet ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Maternal Blood ◽  
Droplet Digital Pcr ◽  
New Approach ◽  
Fetal Dna

scholarly journals Droplet digital PCR combined with minisequencing, a new approach to analyze fetal DNA from maternal blood: application to the non-invasive prenatal diagnosis of achondroplasia

2016 ◽  
Vol 36 (5) ◽  
pp. 397-406 ◽  
Author(s):  
Lucie Orhant ◽  
Olivia Anselem ◽  
Mélanie Fradin ◽  
Pierre Hadrien Becker ◽  
Caroline Beugnet ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Digital Pcr ◽  
Maternal Blood ◽  
Droplet Digital Pcr ◽  
New Approach ◽  
Non Invasive ◽  
Fetal Dna

Comparison of droplet digital PCR with quantitative real-time PCR for determination of zygosity in transgenic maize

2016 ◽  
Vol 25 (6) ◽  
pp. 855-864 ◽  
Author(s):  
Xiaoli Xu ◽  
Cheng Peng ◽  
Xiaofu Wang ◽  
Xiaoyun Chen ◽  
Qiang Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Transgenic Maize ◽  
Quantitative Real Time Pcr

scholarly journals Determination of fetal chromosome aberrations from fetal DNA in maternal blood: has the challenge finally been met?

2011 ◽  
Vol 13 ◽  
Author(s):  
Sinuhe Hahn ◽  
Olav Lapaire ◽  
Sevgi Tercanli ◽  
Varaprasad Kolla ◽  
Irene Hösli
Keyword(s):  
Point Mutations ◽  
Digital Pcr ◽  
Maternal Blood ◽  
Noninvasive Detection ◽  
Current Form ◽  
Targeted Ngs ◽  
The Right ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

The analysis of cell-free fetal nucleic acids in maternal blood for prenatal diagnosis has been transformed by several recent profound technology developments. The most noteworthy of these are ‘digital PCR’ and ‘next-generation sequencing’ (NGS), which might finally deliver the long-sought goal of noninvasive detection of fetal aneuploidy. Recent data, however, indicate that NGS might even be able to offer a much more detailed appraisal of the fetal genome, including paternal and maternal inheritance of point mutations for mendelian disorders such as β-thalassaemia. Although these developments are very exciting, in their current form they are still too complex and costly, and will need to be simplified considerably for their optimal translation to the clinic. In this regard, targeted NGS does appear to be a step in the right direction, although this should be seen in the context of ongoing progress with the isolation of fetal cells and with proteomic screening markers.

Accurate Determination of Plasmid Copy Number of Flow-Sorted Cells using Droplet Digital PCR

2014 ◽  
Vol 86 (12) ◽  
pp. 5969-5976 ◽  
Author(s):  
Michael Jahn ◽  
Carsten Vorpahl ◽  
Dominique Türkowsky ◽  
Martin Lindmeyer ◽  
Bruno Bühler ◽  
...  
Keyword(s):  
Copy Number ◽  
Accurate Determination ◽  
Digital Pcr ◽  
Plasmid Copy Number ◽  
Droplet Digital Pcr ◽  
Plasmid Copy

Droplet digital PCR for the determination of fetal red cell antigens in pregnancy

Pathology ◽  
2018 ◽  
Vol 50 ◽  
pp. S103-S104
Author(s):  
H. O'Brien ◽  
C. Hyland ◽  
E. Schoeman ◽  
G. Millard ◽  
J. Hyett ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Red Cell ◽  
Red Cell Antigens ◽  
In Pregnancy

scholarly journals Droplet Digital PCR for absolute quantification and determination of proviral load of HTLV-1 and 2

Retrovirology ◽  
2015 ◽  
Vol 12 (S1) ◽  
Author(s):  
Sara Thulin Hedberg ◽  
Lorraine Eriksson ◽  
Kerstin Malm ◽  
Paula Mölling ◽  
Martin Sundqvist ◽  
...  
Keyword(s):  
Proviral Load ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Droplet Digital Pcr
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