Pulsed Field Gel Electrophoresis types of Candida albicans isolates from an intensive care unit in a Tunisian hospital

2011 ◽  
Vol 69 (3) ◽  
pp. 289-294
Author(s):  
Nadia Khadraoui ◽  
Kalthoum Kallel ◽  
Ons Bouchami ◽  
Myriam Bouchakoua ◽  
Amira Kaouech ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Candida Albicans ◽  
Intensive Care ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Rapid Eradication of a Cluster ofSerratia marcescensin a Neonatal Intensive Care Unit: Use of Epidemiologic Chromosome Profiling by Pulsed-Field Gel Electrophoresis

2004 ◽  
Vol 25 (9) ◽  
pp. 730-734 ◽  
Author(s):  
Kwan Kew Lai ◽  
Stephen P. Baker ◽  
Sally A. Fontecchio
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Gel Electrophoresis ◽  
Neonatal Intensive Care ◽  
Pulsed Field Gel Electrophoresis ◽  
Control Measures ◽  
Pulsed Field ◽  
Contact Precautions ◽  
Control Study

AbstractObjective:To investigate a cluster of patients infected and colonized withSerratia marcescensin a neonatal intensive care unit (NICU).Methods:In June 2001, two neonates in the NICU had clinical infections withS. marcescensand one died. Infection control surveillance data for the NICU revealed that S.marcescenswas rarely isolated from clinical specimens. Surveillance and environmental cultures were performed and isolates were typed using pulsed-field gel electrophoresis. Staff and neonates were cohorted and a waterless, alcohol-based handwashing agent was introduced. A case-control study was performed.Results:From June 2 through August 20, 2001, 11 neonates withS. marcescensinfection and colonization were identified. The incidence ofS. marcescensinfections increased from 0.19 per 1,000 patient-days in 2000 to 0.52 per 1,000 patient-days in 2001 (P< .0001). In the first 3 weeks of the investigation, there were 2 sets of patients and sinks with indistinguishable strains; however, in subsequent weeks, all isolates were of unique strains, signifying no further transmission of the two initial predominant strains. Neonates withS. marcescenswere more likely to have a lower gestational age and birth weight. There was no association between cases and healthcare workers (HCWs).Conclusions:A cluster ofS. marcescenswas quickly terminated after the introduction of preventive measures including cohorting of infected and colonized neonates and HCWs, contact precautions, surveillance cultures, and a waterless, alcohol-based hand antiseptic. Chromosomal typing determined that strains with an indistinguishable pattern were no longer present in the unit after control measures were implemented.

Outbreak ofSerratia marcescensInfection in a Neonatal Intensive Care Unit

2001 ◽  
Vol 22 (5) ◽  
pp. 303-305 ◽  
Author(s):  
G.A. Prasad ◽  
Paula G. Jones ◽  
Jacqueline Michaels ◽  
Jeffery S. Garland ◽  
Chandra R. Shivpuri
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Serratia Marcescens ◽  
Gel Electrophoresis ◽  
Neonatal Intensive Care ◽  
Community Hospital ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Source Of Infection

AbstractWe report an outbreak ofSerratia marcescensinfection in the neonatal intensive care unit of a community hospital. The outbreak involved eight neonates, (five infected and three colonized), one of whom died. Pulsed-field gel electrophoresis confirmed that all isolates were identical strains. Cohorting and isolation of the infected neonates helped to control the outbreak. No environmental source of infection was found.

An Outbreak Due to Serratia marcescens in a Neonatal Intensive Care Unit Typed by 2-Day Pulsed Field Gel Electrophoresis Protocol

2003 ◽  
Vol 34 (3) ◽  
pp. 237-241 ◽  
Author(s):  
Guadalupe Miranda-Novales ◽  
Blanca Leaños-Miranda ◽  
Rita Dı́az-Ramos ◽  
Lourdes González-Tejeda ◽  
Leoncio Peregrino-Bejarano ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Serratia Marcescens ◽  
Gel Electrophoresis ◽  
Neonatal Intensive Care ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field
Sign in / Sign up

Export Citation Format

Share Document