scholarly journals Light and Electron Microscopic Immunohistochemistry on the Localization of Cytochrome P-450 of the Side Chain Cleavage System and of Cytochrome P-450 of 11β-Hydroxylase in the Bovine Adrenal Cortical Cells

1985 ◽  
Vol 48 (5) ◽  
pp. 541-546 ◽  
Author(s):  
Kazunori ISHIMURA ◽  
Tomoko YOSHINAGA ◽  
Hisao FUJITA ◽  
Sachiko SUGANO ◽  
Mitsuhiro OKAMOTO ◽  
...  
Keyword(s):  
Side Chain ◽  
Electron Microscopic ◽  
Cortical Cells ◽  
Side Chain Cleavage ◽  
Chain Cleavage ◽  
Adrenal Cortical Cells ◽  
Cytochrome P 450

Alteration in the expression of genes for cholesterol side-chain cleavage enzyme and 21-hydroxylase by hypophysectomy and ACTH administration in the rat adrenal

1990 ◽  
Vol 4 (3) ◽  
pp. 239-245 ◽  
Author(s):  
T. Imai ◽  
H. Seo ◽  
Y. Murata ◽  
M. Ohno ◽  
Y. Satoh ◽  
...  
Keyword(s):  
Steady State ◽  
Adrenal Weight ◽  
Side Chain ◽  
Total Rna ◽  
Side Chain Cleavage ◽  
Chain Cleavage ◽  
Acth Treatment ◽  
Steady State Levels ◽  
Cytochrome P 450

ABSTRACT The changes in steady-state levels of mRNA for cholesterol side-chain cleavage cytochrome P-450 (P-450scc) and steroid 21-hydroxylase cytochrome P-450 (P-450c21) caused by hypophysectomy and ACTH treatment were determined in rat adrenals. Hypophysectomy caused marked decreases in adrenal weight and total RNA per gland. Administration of ACTH resulted in increases in adrenal weight and total RNA. A significant correlation between the amount of RNA and adrenal weight was observed. Both P-450scc and P-450c21 mRNAs were decreased by hypophysectomy and increased by ACTH treatment. P-450scc mRNA decreased to 20% and P-450c21 mRNA to 76% of control values 1 day after hypophysectomy. ACTH caused a significant increase in P-450scc mRNA after 3 h. However, a significant increase in P-450c21 mRNA was observed 12 h after administration of ACTH. These results are concordant with previous studies in vitro utilizing cultured adrenocortical cells. Moreover, the induction of steady-state levels of P-450scc mRNA was faster than that observed by other investigators in studies in vitro. These results may indicate that integrity of the adrenal gland in vivo is important for the action of ACTH.

scholarly journals Immunolocalization of cholesterol side-chain-cleavage cytochrome P-450 and 17 -hydroxylase cytochrome P-450 in bovine ovarian follicles

Reproduction ◽  
1986 ◽  
Vol 78 (2) ◽  
pp. 627-638 ◽  
Author(s):  
R. J. Rodgers ◽  
H. F. Rodgers ◽  
P. F. Hall ◽  
M. R. Waterman ◽  
E. R. Simpson
Keyword(s):  
Ovarian Follicles ◽  
Side Chain ◽  
Side Chain Cleavage ◽  
Chain Cleavage ◽  
Cytochrome P 450
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