A STUDY OF THE ACTION OF MIROESTROL AND OTHER OESTROGENS ON THE REPRODUCTIVE TRACT OF THE IMMATURE FEMALE MOUSE

1960 ◽  
Vol 20 (3) ◽  
pp. 229-235 ◽  
Author(s):  
H. E. H. JONES ◽  
G. S. POPE
Keyword(s):  
Latent Period ◽  
Female Mouse ◽  
Reproductive Tract ◽  
Single Injection ◽  
Route Of Administration ◽  
Uterine Weight ◽  
Immature Female ◽  
Uterine Lumen ◽  
Multiple Doses

SUMMARY 1. Assay of the potency of the recently isolated plant oestrogen, miroestrol, shows that when given subcutaneously in multiple doses it is as potent as oestradiol-17β, and orally more than three times as potent as stilboestrol in producing an increase in uterine weight in the immature female mouse. 2. In the immature female mouse given a single injection of oestrogens, the route of administration influences the magnitude, the latent period, and the duration of the responses in the reproductive tract. In general, the oestrogens act most efficiently on the uterus and vagina when they are given intraperitoneally instead of subcutaneously. It is suggested that this is because there is less chance of absorption rates and metabolism in the animal influencing the oestrogen introduced intraperitoneally. 3. The activity of miroestrol by single injection is described and compared with that of oestriol, oestradiol-17β and stilboestrol. It is at least as effective as oestradiol-17β and stilboestrol in promoting uterine and vaginal growth, and in increasing the amount of fluid which can be expressed from the uterine lumen by gentle pressing.

EFFECT OF STEROID HORMONES ON OESTROGEN-INDUCED UTERINE GLYCOGEN SYNTHESIS

1968 ◽  
Vol 42 (1) ◽  
pp. 65-77 ◽  
Author(s):  
HELENE C. CECIL ◽  
J. BITMAN
Keyword(s):  
Single Dose ◽  
Testosterone Propionate ◽  
Single Injection ◽  
Initial Period ◽  
Glycogen Synthesis ◽  
Uterine Weight ◽  
Adult Rats ◽  
Control Value ◽  
Multiple Doses ◽  
Ovariectomized Adult Rats

SUMMARY Uterine weight, water, glycogen and glucose responses were analysed for 96 hr. after a single dose or daily doses of oestradiol given to ovariectomized adult rats. Six hr. after a single dose of oestradiol the uterine glycogen concentration doubled, reached a maximum of 4 times the control value at 12 hr. and then declined during the 16–48 hr. period. The ability of cortisol, deoxycorticosterone, progesterone, 17-ethyl-19-nortestosterone (Nilevar) or testosterone propionate to inhibit these responses was determined at 6 hr. (the initial period of maximum oestrogen action) and at 24 hr. (period of uterine glycogenolysis). A single injection of the antagonist given at the same time as oestrogen had no effect on the 6 hr. glycogen response. Pretreatment with cortisol, progesterone or testosterone propionate for 3 days caused a 30–50% inhibition of the oestrogen-induced increase. Multiple doses of testosterone propionate by itself were glycogenic. Single doses of the compounds appeared to be more effective at 24 hr. Multiple doses of cortisol, deoxycorticosterone, progesterone and testosterone propionate inhibited the 24 hr. glycogen response to oestradiol by 30–50%.

Estrogen-like Activity in Vegetable Oils and Mill By-products

Science ◽  
1960 ◽  
Vol 131 (3416) ◽  
pp. 1807-1808 ◽  
Author(s):  
A. N. Booth ◽  
E. M. Bickoff ◽  
G. O. Kohler
Keyword(s):  
Vegetable Oils ◽  
Female Mouse ◽  
Mouse Bioassay ◽  
Uterine Weight ◽  
Immature Female ◽  
Bioassay Technique ◽  
By Products

By the immature female mouse bioassay technique, an increased uterine weight was observed when certain vegetable oils were fed or injected. Byproducts from the milling of cereals were also capable of eliciting a uterine response.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

AUGMENTATION BY CHLORMADINONE OF THE UTERINE WEIGHT RESPONSE TO HUMAN CHORIONIC GONADOTROPHIN IN INTACT IMMATURE RATS

1965 ◽  
Vol 33 (3) ◽  
pp. 447-454
Author(s):  
M. J. K. HARPER
Keyword(s):  
Single Injection ◽  
Direct Action ◽  
Follicular Development ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Female Rats ◽  
Uterine Weight ◽  
Control Value ◽  
Orally Active ◽  
Stimulation Of

SUMMARY Administration of chlormadinone, an orally active progestational agent without significant oestrogenic activity, to intact immature female rats did not affect either ovarian or uterine weight significantly compared with controls. A single injection of human chorionic gonadotrophin (HCG) caused a 73 % increase in uterine weight in 24 hr. over the control value. This dose significantly increased ovarian weight and although it caused some stimulation of follicular development, ovulation during this time did not occur. When animals were treated with chlormadinone for 8 days, and received HCG on the 8th day, uterine weight was 170% greater than in the controls and 56% greater than with HCG alone. The uterine weight produced was similar to that found in animals treated with mestranol, a potent oestrogen, and HCG. In ovariectomized animals HCG did not affect uterine weight, while the small increase produced by chlormadinone was unaltered when HCG also was given. Mechanisms are discussed by which this augmentation of the uterine response to HCG might be produced. It seems most likely that chlormadinone administration causes storage of endogenous gonadotrophin in the pituitary, and that the exogenous gonadotrophin acts as the 'trigger' for the release of stored hormone, probably by a direct action on the hypothalamus.

Further Observations upon the Oxyntic Cells with Special Reference to Acid Phosphatase

1952 ◽  
Vol s3-93 (23) ◽  
pp. 259-268
Author(s):  
GORDON MENZIES
Keyword(s):  
Acid Phosphatase ◽  
Special Reference ◽  
Single Injection ◽  
Central Area ◽  
Basal Part ◽  
Prolonged Starvation ◽  
Apparent Change ◽  
Multiple Doses ◽  
Hydrolysis Of ◽  
In Cells

1. In continuation of work already reported (Menzies, 1949) further data are presented on the structure and cyto-chemistry of the granules in the oxyntic cells of the rat's stomach. 2. After multiple doses of pilocarpine or histamine, and after feeding, the phospholipine (as shown by acid haematein) is shed from some or all of the granules. The lipine first leaves the granules in cells situated in the basal part of the tubules, and finally those in the neck of the tubules, but a non-lipine lipoid remains in all the granules (as shown by sudan black). 3. The granules enlarge after prolonged starvation as they do after a single injection of pilocarpine; but after extraction of lipoids by hot pyridine and subsequent straining with iron haematoxylin, enlargement (i.e. of the non-lipoid moiety) is shown only after prolonged starvation. 4. A light, uncoloured central area is found in some of the largest granules after feeding and colouring with acid haematein. 5. An acid phosphatase appears in the oxyntic cells whose granules are about to lose their lipine component, and it disappears when they have done so. It is suggested that the acid phosphatase may cause hydrolysis of the phospholipine. 6. Only after prolonged starvation is there any apparent change in granule numbers, when they are decreased.

Effects of acute administration of 2-hydroxylated metabolites of oestrogens on LH and prolactin secretion in male and female prepubertal rats

1984 ◽  
Vol 103 (3) ◽  
pp. 317-325
Author(s):  
A. K. Brar ◽  
G. Fink
Keyword(s):  
Plasma Concentration ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Female Rat ◽  
Uterine Weight ◽  
Immature Female ◽  
Male And Female ◽  
Immature Male ◽  
Lh Release

ABSTRACT The effects of catechol oestradiol and catechol oestrone on the release of LH and prolactin were investigated in immature male and female Wistar rats. In male rats both catechol oestradiol and catechol oestrone significantly increased the plasma concentration of LH, and catechol oestradiol but not catechol oestrone significantly increased the plasma concentration of prolactin and decreased the pituitary concentration of LH. The parent oestrogens, oestradiol-17β and oestrone, had no effect on plasma LH concentrations, but both increased significantly the plasma concentration of prolactin, and oestrone but not oestradiol-17β increased the pituitary concentration of LH. In immature female rats, catechol oestradiol inhibited the surge of LH and the increase in uterine weight induced by injecting pregnant mare serum gonadotrophin (PMSG). The injection of oestrone induced an increase in the plasma concentration of LH which was about nine times greater than that produced by oestradiol-17β. There were no significant differences in the effects of these steroids on plasma prolactin concentration. These results (i) confirm that in the immature male rat catechol oestrogens can stimulate LH release and show that catechol oestradiol can increase prolactin release, (ii) show that catechol oestradiol can inhibit the stimulatory effects of PMSG on LH release and uterine weight in the immature female rat, and (iii) demonstrate that oestrone can stimulate LH release in the immature female rat. J. Endocr. (1984) 103, 317-325

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