scholarly journals Differential effects of sex steroid hormones on the expression of multiple first exons including a novel first exon of prolactin receptor gene in the rat liver

2005 ◽  
Vol 34 (3) ◽  
pp. 667-673 ◽  
Author(s):  
M Tanaka ◽  
M Suzuki ◽  
T Kawana ◽  
M Segawa ◽  
M Yoshikawa ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Rat Liver ◽  
Prolactin Receptor ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Male Rat ◽  
Female Rat ◽  
R Gene ◽  
Rt Pcr ◽  
Differential Effects

In addition to the known four alternative first exons E11, E12, E13 and E14 of the rat prolactin receptor (PRL-R) gene, a novel first exon, E15, was identified by cDNA cloning of the 5′-end region of PRL-R mRNA in the rat liver. Genomic fragments containing E15 and its 5′- or 3′-flanking regions were also cloned from rat kidney genomic DNA. A sequence search for E15 revealed that E15 is located 49 kb upstream of exon 2 of the PRL-R gene in rat chromosome 2q16. RT-PCR analysis revealed that E15 was preferentially expressed in the liver, brain and kidney. Expression profiles of E12-, E13- and E15-PRL-R mRNAs in the liver of male and female rats at 5 days of age and those at 8 weeks of age were examined by RT-PCR. The levels of E12-PRL-R mRNA in the female rat increased remarkably in rats at 8 weeks of age compared with those at 5 days of age, and the levels of E15-PRL-R mRNA in the male rat decreased markedly at 8 weeks of age compared with those at 5 days of age. In the female rat, the levels of E12-PRL-R mRNA at 8 weeks of age decreased with ovariectomy performed at 4 weeks of age and recovered with the administration of β-oestradiol. On the contrary, the levels of E15-PRL-R mRNA increased with ovariectomy and decreased with the oestrogen treatment. In the male rat liver, the levels of E12-PRL-R mRNA at 8 weeks of age increased strikingly with castration performed at 4 weeks of age and became undetectable with the administration of testosterone. The levels of E15-PRL-R mRNA increased slightly with castration and were restored by testosterone treatment. Removal of gonadal tissues and sex steroid hormone treatment had no effect on the expression levels of E13-PRL-R mRNA in both female and male rat livers. These results indicated that the expression of the PRL-R gene in the liver is regulated by the differential effects of sex steroid hormones on the transcription of the multiple first exons including the novel one.

scholarly journals Vitellogenin Gene Expression and Sex Steroid Levels as Biomarkers in Yellowfin Seabream (Acanthopagrus latus) Exposed to Bisphenol-A

2019 ◽  
Vol 13 (1) ◽  
pp. 27-33
Author(s):  
Ahmad Negintaji ◽  
◽  
Alireza Safahieh ◽  
Hosein Zolgharnein ◽  
Soheila Matroodi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bisphenol A ◽  
Steroid Hormones ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Plasma Testosterone ◽  
Dependent Manner ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Acanthopagrus Latus

Background: The egg yolk precursor protein vitellogenin (VTG) has proven to be a useful biomarker, used to identify organisms exposed to estrogenic compounds. Methods: We investigated variations in the VTG gene expression pattern and plasma sex steroid hormones concentrations in the yellowfin Seabream, Acanthopagrus latus, (A. latus) by various doses of bisphenol-A (BPA) exposure for 7 and 14 days. We developed a quantitative real time polymerase chain reaction (RT-PCR) assay for the expression of VTG gene in A. latus. The dose-response pattern of VTG gene expression in A. latus exposed to various doses of BPA was characterized. In order to design RT-PCR primers specific to A. latus VTG, a partial sequence of the VTG gene was obtained. Results: The RT-PCR assay was effective in detecting increased VTG gene expression in A. latus exposed to BPA. It also demonstrated that the VTG expression was affected by BPA in a dose and time-dependent manner. Plasma testosterone (T) levels were decreased in the treated fish in comparison with those found in the control group, when they were exposed to 100 µg/g of BPA and 2 µg/g of E2. In contrast, the plasma levels of 17β-estradiol (E2) were significantly increased in a dose-dependent manner. Conclusion: The results suggest that VTG mRNA quantification can provide a sensitive and early signal in the detection of estrogens in marine wildlife. It also indicated that BPA could lead to an imbalance of sex steroid hormones with potentially harmful consequences on sexually immature male A. latus.

Immunoreactive Neurotensin in Gonadotrophs and Thyrotrophs is Regulated by Sex Steroid Hormones in the Female Rat

2001 ◽  
Vol 11 (10) ◽  
pp. 785-794 ◽  
Author(s):  
Bello ◽  
Hernández ◽  
González ◽  
Reyes ◽  
Negrín ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Female Rat

Differential modulation of [3H]flunitrazepam binding in female rat brain by sex steroid hormones

1989 ◽  
Vol 170 (1-2) ◽  
pp. 95-99 ◽  
Author(s):  
Marcello Canonaco ◽  
Antonella Valenti ◽  
Renata Tavolaro ◽  
Ezio Bettini ◽  
Adriana Maggi
Keyword(s):  
Rat Brain ◽  
Steroid Hormones ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Female Rat ◽  
Differential Modulation

scholarly journals Associations between female lung cancer risk and sex steroid hormones: a systematic review and meta-analysis of the worldwide epidemiological evidence on endogenous and exogenous sex steroid hormones

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hui Zeng ◽  
Zhuoyu Yang ◽  
Jiang Li ◽  
Yan Wen ◽  
Zheng Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Risk ◽  
Steroid Hormones ◽  
Lung Cancer Risk ◽  
Meta Analysis ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Sex Steroid Hormone ◽  
Hormone Exposure ◽  
Female Lung Cancer

Abstract Background Published findings suggest sex differences in lung cancer risk and a potential role for sex steroid hormones. Our aim was to perform a meta-analysis to investigate the effects of sex steroid hormone exposure specifically on the risk of lung cancer in women. Methods The PubMed, MEDLINE, Web of Science, and EMBASE databases were searched. The pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) for female lung cancer risk associated with sex steroid hormones were calculated overall and by study design, publication year, population, and smoking status. Sensitivity analysis, publication bias, and subgroup analysis were performed. Results Forty-eight studies published between 1987 and 2019 were included in the study with a total of 31,592 female lung cancer cases and 1,416,320 subjects without lung cancer. Overall, higher levels of sex steroid hormones, both endogenous (OR: 0.92, 95% CI: 0.87–0.98) and exogenous (OR: 0.86, 95% CI: 0.80–0.93), significantly decreased the risk of female lung cancer by 10% (OR: 0.90, 95% CI: 0.86–0.95). The risk of lung cancer decreased more significantly with a higher level of sex steroid hormones in non-smoking women (OR: 0.88, 95% CI: 0.78–0.99) than in smoking women (OR: 0.98, 95% CI: 0.77–1.03), especially in Asia women (OR: 0.84, 95% CI: 0.74–0.96). Conclusions Our meta-analysis reveals an association between higher levels of sex steroid hormone exposure and the decreased risk of female lung cancer. Surveillance of sex steroid hormones might be used for identifying populations at high risk for lung cancer, especially among non-smoking women.

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