scholarly journals Identification of Suitable Reference Genes for gene Expression Studies by qRT-PCR in the Blister BeetleMylabris cichorii

2014 ◽  
Vol 14 (94) ◽  
pp. 1-10 ◽  
Author(s):  
Yu Wang ◽  
Zhong-Kang Wang ◽  
Yi Huang ◽  
Yu-Feng Liao ◽  
You-Ping Yin
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Qrt Pcr ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Suitable Reference

Determination of Suitable Candidate Genes as Endogenous Control for Gene Expression Analysis in Local Capsicum Annuum MC11

2021 ◽  
Vol 12 (3) ◽  
pp. 1011-1017
Author(s):  
Marina Mokhtar Et.al
Keyword(s):  
Gene Expression ◽  
Candidate Genes ◽  
Capsicum Annuum ◽  
Reference Genes ◽  
Data Normalization ◽  
Plant Parts ◽  
Qrt Pcr ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Suitable Reference

Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for qRT-PCR assays. This study identifies suitable reference genes for local chilli, Capsicum annuum var MC11 under incident of Cucumber mosaic virus infection. Six candidate genes actin, tub, EF1α, GAPDH, TEF1α and 18SrRNA and three validated Capsicum reference genes UBI-3 ref, β-tub ref and gapdhref were tested against five chilli plant parts stem, shoot, leave, flower and root.  The PCR/qRT-PCR results demonstrate only five candidate references genes actin, EF1α, GAPDH, 18SrRNA, and TEF1α that show specific single band of amplicon, without primer dimers and at the targeted sizes. Through qRT-PCR, GAPDH gives single peak in dissociation curve in all plant parts used further fulfilling the characteristic of reference genes.Previous work on validation of reference genes in pepper shows that only UBI-3 suits to C. annuum var MC11 infected CMV, thus we suggest that GAPDH has a potential to be a validated reference gene for C. annuum var MC11 and can be used together UBI-3 for the purpose of data normalization. 

scholarly journals Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression inDioscorea opposita

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Xiting Zhao ◽  
Xiaoli Zhang ◽  
Xiaobo Guo ◽  
Shujie Li ◽  
Linlin Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Developmental Stages ◽  
Chain Reaction ◽  
Qrt Pcr ◽  
Expression Studies ◽  
Tuber Crop ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
Suitable Reference

Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for obtaining reliable results for qRT-PCR assays. This study evaluated potential reference genes of Chinese yam (Dioscorea oppositaThunb.), which is an important tuber crop and medicinal plant in East Asia. The expression of ten candidate reference genes across 20 samples from different organs and development stages was assessed. We identified the most stable genes for qRT-PCR studies using combined samples from different organs. Our results also suggest that different suitable reference genes or combinations of reference genes for normalization should be applied according to different organs and developmental stages. To validate the suitability of the reference genes, we evaluated the relative expression ofPE2.1andPE53, which are two genes that may be associated with microtuber formation. Our results provide the foundation for reference gene(s) selection inD. oppositaand will contribute toward more accurate gene analysis studies of the genusDioscorea.

scholarly journals In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR

2007 ◽  
Vol 8 (1) ◽  
pp. 47 ◽  
Author(s):  
Monika Jung ◽  
Azizbek Ramankulov ◽  
Jan Roigas ◽  
Manfred Johannsen ◽  
Martin Ringsdorf ◽  
...  
Keyword(s):  
Gene Expression ◽  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Real Time Pcr ◽  
Renal Cell ◽  
Reference Genes ◽  
Human Renal Cell Carcinoma ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Suitable Reference

scholarly journals Gene expression studies in different genotypes of an ectomycorrhizal fungus require a high number of reliable reference genes

2016 ◽  
Author(s):  
Joske Ruytinx ◽  
Tony Remans ◽  
Jan V Colpaert
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Intraspecific Variability ◽  
Standard Technique ◽  
Housekeeping Genes ◽  
Ectomycorrhizal Fungus ◽  
Suillus Luteus ◽  
Qrt Pcr ◽  
Expression Studies ◽  
Gene Expression Studies

Quantitative reverse transcription PCR (qRT-PCR) has become the standard technique for the expression analysis of a set of chosen genes of interest. The accuracy and reliability of qRT-PCR measurements strongly depends on the normalization with appropriate endogenous reference genes. In this study a set of candidate reference genes for the use in gene expression studies of a basidiomycete fungus, Suillus luteus, exposed to toxic concentrations of zinc or cadmium was identified, evaluated and validated. Seven candidate genes were selected from cDNA-AFLP as stably expressed and the algorithms geNorm and Normfinder were used to evaluate these genes alongside the traditionally used housekeeping genes (actin, tubulin) in different S. luteus isolates. The use of several S. luteus isolates revealed that each isolate has its own most stably expressed set of reference genes, regardless of the metal treatments, in casu metal exposures. Metal treatments had only a minor impact on the expression of the candidate reference genes. The validated reference genes outperform the in fungal research commonly used single, arbitrary chosen (“housekeeping”) genes in terms of reliability, and have the potential to be suitable reference genes when studying the effect of other environmental factors. A relatively high number of reference genes is required to correct for intraspecific variability when studying natural populations.

Sign in / Sign up

Export Citation Format

Share Document