scholarly journals Serum-Free Culture of the Suspension Cell Line QB-Tn9-4s of the Cabbage Looper,Trichoplusia ni, is Highly Productive for Virus Replication and Recombinant Protein Expression

2014 ◽  
Vol 14 (24) ◽  
pp. 1-14
Author(s):  
Gui-Ling Zheng ◽  
Hong-Xu Zhou ◽  
Chang-You Li
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Cell Line ◽  
Virus Replication ◽  
Trichoplusia Ni ◽  
Recombinant Protein Expression ◽  
Suspension Cell ◽  
Cabbage Looper ◽  
Serum Free ◽  
Suspension Cell Line

Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines

2018 ◽  
Vol 185 (4) ◽  
pp. 986-1003 ◽  
Author(s):  
Seyedeh Hoda Jazayeri ◽  
Amir Amiri-Yekta ◽  
Salahadin Bahrami ◽  
Hamid Gourabi ◽  
Mohammad Hossein Sanati ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Recombinant Protein Expression ◽  
Mammalian Cell Lines ◽  
Line Engineering

scholarly journals Genome Assembly and Annotation of the Trichoplusia ni Tni-FNL Insect Cell Line Enabled by Long-Read Technologies

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 79 ◽  
Author(s):  
Keyur Talsania ◽  
Monika Mehta ◽  
Castle Raley ◽  
Yuliya Kriga ◽  
Sujatha Gowda ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Cell Line ◽  
Genome Assembly ◽  
Insect Cell ◽  
Trichoplusia Ni ◽  
Genome Engineering ◽  
Insect Cell Line ◽  
Baculovirus Infection ◽  
Long Read

Background: Trichoplusia ni derived cell lines are commonly used to enable recombinant protein expression via baculovirus infection to generate materials approved for clinical use and in clinical trials. In order to develop systems biology and genome engineering tools to improve protein expression in this host, we performed de novo genome assembly of the Trichoplusia ni-derived cell line Tni-FNL. Methods: By integration of PacBio single-molecule sequencing, Bionano optical mapping, and 10X Genomics linked-reads data, we have produced a draft genome assembly of Tni-FNL. Results: Our assembly contains 280 scaffolds, with a N50 scaffold size of 2.3 Mb and a total length of 359 Mb. Annotation of the Tni-FNL genome resulted in 14,101 predicted genes and 93.2% of the predicted proteome contained recognizable protein domains. Ortholog searches within the superorder Holometabola provided further evidence of high accuracy and completeness of the Tni-FNL genome assembly. Conclusions: This first draft Tni-FNL genome assembly was enabled by complementary long-read technologies and represents a high-quality, well-annotated genome that provides novel insight into the complexity of this insect cell line and can serve as a reference for future large-scale genome engineering work in this and other similar recombinant protein production hosts.

Recombinant protein expression in aDrosophila cell line: comparison with the baculovirus system

1994 ◽  
Vol 15 (1-3) ◽  
pp. 139-144 ◽  
Author(s):  
A. R. Bernard ◽  
T. A. Kost ◽  
L. Overton ◽  
C. Cavegn ◽  
J. Young ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Protein Expression ◽  
Cell Line ◽  
Recombinant Protein Expression ◽  
Baculovirus System
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