scholarly journals Molecular Cloning, Expression Profile and 5′ Regulatory Region Analysis of Two Chemosensory Protein Genes from the Diamondback Moth,Plutella xylostella

2010 ◽  
Vol 10 (143) ◽  
pp. 1-15 ◽  
Author(s):  
Liang Gong ◽  
Guo-Hua Zhong ◽  
Mei-Ying Hu ◽  
Qian Luo ◽  
Zhen-Zhen Ren
Keyword(s):  
Molecular Cloning ◽  
Expression Profile ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Regulatory Region ◽  
Region Analysis ◽  
Chemosensory Protein

scholarly journals Expression Profiles and Binding Properties of the Chemosensory Protein PxylCSP11 from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

2020 ◽  
Vol 20 (5) ◽  
Author(s):  
Shuhui Fu ◽  
Fangyuan Li ◽  
Xizhong Yan ◽  
Chi Hao
Keyword(s):  
Plutella Xylostella ◽  
Expression Profiles ◽  
Diamondback Moth ◽  
Expression Patterns ◽  
Host Recognition ◽  
Limited Information ◽  
Host Volatiles ◽  
Binding Characteristics ◽  
Chemosensory Protein ◽  
Cruciferous Plants

Abstract The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is one of the most destructive pests to cruciferous plants worldwide. The oligophagous moth primarily utilizes its host volatiles for foraging and oviposition. Chemosensory proteins (CSPs) are soluble carrier proteins with low molecular weight, which recognize and transport various semiochemicals in insect chemoreception. At present, there is limited information on the recognition of host volatiles by CSPs of P. xylostella. Here, we investigated expression patterns and binding characteristics of PxylCSP11 in P. xylostella. The open reading frame of PxylCSP11 was 369-bp encoding 122 amino acids. PxylCSP11 possessed four conserved cysteines, which was consistent with the typical characteristic of CSPs. PxylCSP11 was highly expressed in antennae, and the expression level of PxylCSP11 in male antennae was higher than that in female antennae. Fluorescence competitive binding assays showed that PxylCSP11 had strong binding abilities to several ligands, including volatiles of cruciferous plants, and (Z)-11-hexadecenyl acetate (Z11-16:Ac), a major sex pheromone of P. xylostella. Our results suggest that PxylCSP11 may play an important role in host recognition and spouse location in P. xylostella.

Molecular cloning and expression profiles of calreticulin gene from the diamondback moth, Plutella xylostella

2010 ◽  
Vol 40 (4) ◽  
pp. 217-224 ◽  
Author(s):  
Rye Jin KIM ◽  
Seung Han OH ◽  
Mi Young NOH ◽  
Yong Hun JO ◽  
Seongon KIM ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Plutella Xylostella ◽  
Expression Profiles ◽  
Diamondback Moth ◽  
Cloning And Expression

scholarly journals Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

2013 ◽  
Vol 14 (11) ◽  
pp. 22891-22905 ◽  
Author(s):  
Lixia Tian ◽  
Jiaqiang Yang ◽  
Wenjie Hou ◽  
Baoyun Xu ◽  
Wen Xie ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
P Glycoprotein

Storage Proteins of Diamondback Moth, Plutella xylostella: Purification and Expression Profile

2006 ◽  
Vol 9 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Ahmed M.A. Ibrahim ◽  
Keewoo Lee ◽  
Hyuksoo Lee ◽  
Yonggyun Kim
Keyword(s):  
Expression Profile ◽  
Plutella Xylostella ◽  
Storage Proteins ◽  
Diamondback Moth
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