Development and Cross-Amplification of Microsatellite Markers for Micrurus surinamensis (Elapidae)

2021 ◽  
Vol 55 (1) ◽  
Author(s):  
Anita de Moura Pessoa ◽  
Mariana Pires de Campos Telles ◽  
Cintia Pelegrineti Targueta ◽  
Steven Douglas Aird ◽  
Nelson Jorge da Silva
Keyword(s):  
Microsatellite Markers ◽  
Cross Amplification

scholarly journals Cross-amplification of heterologous microsatellite markers in Piracanjuba

2017 ◽  
Vol 47 (12) ◽  
Author(s):  
Pedro Luiz de Castro ◽  
Ricardo Pereira Ribeiro ◽  
Silvio Carlos Alves dos Santos ◽  
Elenice Souza dos Reis Goes ◽  
Felipe Pinheiro de Souza ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Microsatellite Markers ◽  
Oreochromis Niloticus ◽  
Genetic Parameters ◽  
Economic Value ◽  
Current Analysis ◽  
Genetic Studies ◽  
Colossoma Macropomum ◽  
Piaractus Mesopotamicus ◽  
Cross Amplification

ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.

scholarly journals Isolation, characterization, and cross-amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex

2011 ◽  
Vol 98 (10) ◽  
pp. e277-e279 ◽  
Author(s):  
Raquel A. Kriedt ◽  
Aline M. C. Ramos-Fregonezi ◽  
Luciano B. Beheregaray ◽  
Sandro L. Bonatto ◽  
Loreta B. Freitas
Keyword(s):  
Microsatellite Markers ◽  
Cross Amplification ◽  
Petunia Integrifolia
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