Increasing Pond Density to Maintain a Patchy Habitat Network of the European Treefrog (Hyla arborea)

2015 ◽  
Vol 49 (2) ◽  
pp. 217-221 ◽  
Author(s):  
Gwenaëlle Le Lay ◽  
Sonia Angelone ◽  
Rolf Holderegger ◽  
Christoph Flory ◽  
Janine Bolliger
Keyword(s):  
Habitat Network ◽  
Hyla Arborea ◽  
Patchy Habitat

scholarly journals Support for a role of colour vision in mate choice in the nocturnal European treefrog (Hyla arborea)

Behaviour ◽  
2011 ◽  
Vol 148 (3) ◽  
pp. 403-404
Author(s):  
Doris Gomez ◽  
Sandrine Plenet ◽  
Thierry Lengagne ◽  
Maxime Derex ◽  
Jean-Paul Léna ◽  
...  
Keyword(s):  
Mate Choice ◽  
Colour Vision ◽  
Hyla Arborea

Construction of individual ddRAD libraries v2

2021 ◽  
Author(s):  
Claire Daguin Thiebaut ◽  
Stephanie Ruault ◽  
Charlotte Roby ◽  
Thomas Broquet ◽  
Frédérique Viard ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Sex Chromosome ◽  
De Novo ◽  
Pcr Amplification ◽  
Restriction Enzymes ◽  
Tree Frog ◽  
Size Selection ◽  
Hyla Arborea ◽  
Sequencing Method ◽  
Sample Representation

This protocol describes a double digested restriction-site associated DNA (ddRADseq) procedure, that is a variation on the original RAD sequencing method (Davey & Blaxter 2011), which is used for de novo SNP discovery and genotyping. This protocol differs from the original ddRADseq protocol (Peterson et al 2012), in which the samples are pooled just after the ligation to adaptors (i.e. before size selection and PCR). The present ddRAD protocol as been slightly adapted from Alan Brelsford's protocol published in the supplementary material of this paper: Brelsford, A., Dufresnes, C. & Perrin, N. 2016. High-density sex-specific linkage maps of a European tree frog (Hyla arborea) identify the sex chromosome without information on offspring sex. Heredity 116, 177–181 (2016). https://doi.org/10.1038/hdy.2015.83 In the present protocol, all samples are treated separately, in a microplate, until final PCR amplification performed before pooling. Despite being slightly more costly and time-consuming in the lab, it allows for fine adjustement of each sample representation in the final library pool, ensuring similar number of sequencing reads per sample in the final dataset. Briefly, genomic DNA from the samples are individually digested with 2 restriction enzymes (one rare-cutter and one more frequent cutter) then ligated to a barcoded adaptor (among 24 available) at one side, and a single adaptor at the other side, purified with magnetic beads, and PCR-amplified allowing the addition of a Illumina index (among 12 available) for multiplexing a maximum of 288 sample per library. Samples are then pooled in equimolar conditions after visualisation on an agarose gel. Purification and size selection is then performed before final quality control of the library and sequencing.

POLLINATION AND GENE FLOW IN WHITE CLOVER, GROWING IN A PATCHY HABITAT

2001 ◽  
pp. 35-40 ◽  
Author(s):  
J.L. Osborne ◽  
I.H. Williams ◽  
A.H. Marshall ◽  
T.P.T. Michaelson-Yeates
Keyword(s):  
Gene Flow ◽  
White Clover ◽  
Patchy Habitat

scholarly journals Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine

2012 ◽  
Vol 46 (3) ◽  
pp. e-1-e-9 ◽  
Author(s):  
R. Svitin ◽  
Y. Kuzmin
Keyword(s):  
Host Species ◽  
Triturus Cristatus ◽  
New Host ◽  
Hyla Arborea ◽  
First Time ◽  
New Host Records

Oswaldocruzia duboisi(Nematoda, Molineidae): Morphology, Hosts and Distribution in UkraineOswaldocruzia duboisiBen Slimane, Durette-Desset et Chabaud, 1993 previously known from France and Bulgaria is reported from Ukraine for the first time. The species was found in the material from 8 amphibian host species, of whichLissotriton montadoni, Triturus cristatus, Mesotriton alpestris, Pelophylax ridibunda, P. lessonae, andHyla arboreaare new host records. Newts (Salamandridae) and green frogs (Pelophylax) are considered to be typical hosts forO. duboisi. Illustrated morphological redescription ofO. duboisibased on 141 specimens from various hosts is presented.

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