scholarly journals Translocations in Spleen Cells from Adult Mice Irradiated as Fetuses are Infrequent, but Often Clonal in Nature

2012 ◽  
Vol 178 (6) ◽  
pp. 600-603 ◽  
Author(s):  
Mimako Nakano ◽  
Yoshiaki Kodama ◽  
Kazuo Ohtaki ◽  
Nori Nakamura
Keyword(s):  
Spleen Cells ◽  
Adult Mice

Thymostimulin effects on lymphoid organs in Ames dwarf mice

1993 ◽  
Vol 128 (1) ◽  
pp. 74-80
Author(s):  
Maria A Villanua ◽  
Agnieszka Szary ◽  
Ana I Esquifino ◽  
Andrzej Bartke
Keyword(s):  
Immune Function ◽  
Lymphoid Organs ◽  
Plasma Prolactin ◽  
Nk Activity ◽  
Spleen Cells ◽  
Adult Mice ◽  
Ames Dwarf Mice ◽  
Ames Dwarf ◽  
Spleen Lymphocytes ◽  
Dwarf Mice

This work was undertaken to study the effects of thymostimulin (TP-1) on the immune function in Ames dwarf mice, and to relate these effects to PRL and/or GH deficiency in these animals. Male Ames dwarf mice implanted with pituitaries from normal mice under the kidney capsule, sham-operated dwarf mice and normal immature or adult mice were injected daily for five days with TP-1. In comparison to normal animals, sham-operated dwarf mice had markedly lower body, thymus and spleen weights, as well as a lower number of lymphocytes in the spleen and in the thymus and the natural killer (NK) activity of spleen lymphocytes. Ectopic pituitary transplants produced the expected enhancement of body weight gain and increased spleen and thymus weights, which reached the values found in normal (non-dwarf) animals. The numbers of lymphocytes in the spleen and thymus were significantly increased in pituitary-grafted dwarf mice, but the grafts did not modify the cytotoxic activity of NK spleen cells, or the number of peripheral white blood cells (PWBC). In sham-operated dwarf mice, TP-1 treatment did not modify the number of cells in the spleen and thymus, or the NK activity. In pituitary-grafted dwarf mice, treatment with TP-1 induced an increase in the number of spleen lymphocytes and in the NK activity of spleen cells without affecting the weight of lymphoid organs or the number of thymic cells. Plasma prolactin (PRL) and growth hormone (GH) levels of pituitary-grafted dwarf mice were not changed after TP-1 administration. Surprisingly, the NK activity of spleen lymphocytes in normal adult mice was greatly increased after TP-1 administration. These findings suggest that the thymic extract TP-1 can exert a major stimulatory influence on NK activity of spleen lymphocytes in adult mice, and potentiate some of the stimulatory effects of hormones secreted by ectopic pituitary transplants on the immune function of Ames dwarf mice. These effects are not mediated by modifications of the release of PRL or GH.

scholarly journals IMMUNOCOMPETENCE OF SPLEEN CELLS FROM NEONATALLY THYMECTOMIZED MICE CONFERRED IN VITRO BY A SYNGENEIC THYMUS EXTRACT

1969 ◽  
Vol 130 (4) ◽  
pp. 765-775 ◽  
Author(s):  
Nathan Trainin ◽  
Myra Small ◽  
Amiela Globerson
Keyword(s):  
Host Response ◽  
Protein Concentration ◽  
Lymphoid Cells ◽  
Immune Reactivity ◽  
Spleen Cells ◽  
Graft Versus Host ◽  
Thymus Extract ◽  
Adult Mice ◽  
Thymectomized Mice

Impaired immunological competence of spleen cells from neonatally thymectomized C57B1/6 young adult mice was apparent when these cells were tested in an in vitro graft-versus-host assay. Spleen cell inocula prepared from thymectomized mice did not induce enlargement of (C3H/eb x C57BI/6)F1 newborn spleen explants, whereas the same number of cells from intact donors consistently initiated splenomegaly. Spleen enlargement was observed, however, when the explants were challenged by cells from thymectomized donors in the presence of syngeneic thymus extract, indicating that the spleen cells in suspension attained immunological competence under the influence of a non-cellular component of the thymus. Immunocompetence was also evident when the cells from thymectomized donors were first incubated with thymus extract for 1 hr and subsequently tested for reactivity. Cells from the same thymectomized donor mice exposed in parallel to extracts from syngeneic spleen or mesenteric lymph node at an equivalent protein concentration did not initiate a graft-versus-host response. These experiments demonstrate that immune reactivity in the graft-versus-host response involves activation of lymphoid cells by a humoral factor of the thymus acting directly upon these cells.

scholarly journals Monoclonal antibodies reactive with the mouse interleukin 5 receptor.

1989 ◽  
Vol 169 (5) ◽  
pp. 1693-1701 ◽  
Author(s):  
A G Rolink ◽  
F Melchers ◽  
R Palacios
Keyword(s):  
B Lymphocytes ◽  
Cell Lines ◽  
Binding Sites ◽  
Lymphoid Cells ◽  
Sensitive Cell ◽  
Spleen Cells ◽  
Interleukin 5 ◽  
Cell Responses ◽  
Adult Mice ◽  
Proliferative Cell

The rat mAbs R52.120 and R52.625 inhibit the action of IL-5 on both IL-5-sensitive cell lines and freshly isolated splenic B lymphocytes. Neither antibody inhibits the proliferative cell responses promoted by IL-2, IL-3, or IL-4. Purified R52.120+ lymphoid spleen cells contain 15-20-fold higher numbers of B lymphocytes responding to IL-5 in the form of maturation into antibody-producing cells. By immunofluorescence staining and flow fluorocytometry, the R52.120 and R52.625 antibodies bound to all 12 IL-5-sensitive cell lines tested. Both antibodies react with 2-4% cells in the spleen, 5% lymphoid cells, and 10-15% myeloid cells in the bone marrow, and 10-14% in the peritoneum of C57BL/6, DBA/2, and BALB/c adult mice. No positive cells for either antibody were detected in the thymus and lymph nodes of these mice. Both R52.120 and R52.625 antibodies specifically inhibit the binding of radiolabeled IL-5 to its receptor. Finally, R52.120 and R52.625 antibodies precipitate from 35S-methionine-labeled IL-5-R+ cell lysates three proteins with Mr 46,000, 130,000, and 140,000. Taken together from these results, we conclude that the R52.120 and R52.625 mAbs recognize epitopes on the IL-5-R complex very close or identical to the IL-5 binding sites.

scholarly journals Immunological responsiveness of neonatal A/J mice to isotypic determinants of syngeneic IgE.

1988 ◽  
Vol 168 (2) ◽  
pp. 713-724 ◽  
Author(s):  
S Haba ◽  
A Nisonoff
Keyword(s):  
T Cells ◽  
B Cells ◽  
Adoptive Transfer ◽  
Time Dependent ◽  
Spleen Cells ◽  
Ige Antibodies ◽  
Neonatal Mice ◽  
Adult Mice ◽  
Range Of Values ◽  
Tolerant State

We have previously shown that adult A/J mice produce high titers of anti-IgE with isotypic or idiotypic specificities in response to challenge with a conjugate of KLH with syngeneic monoclonal IgE. Thus, B cells that can synthesize anti-IgE are present in the mice. Adult mice are unresponsive to unconjugated IgE in CFA, suggesting that tolerance exists at the level of T cells. The present study shows that neonatal mice produce anti-IgE antibodies in response to unconjugated IgE in CFA, but that this capacity is lost after the age of 2-3 wk. The loss of responsiveness corresponds closely with the appearance of detectable IgE in serum, suggesting that the IgE may induce tolerance. The affinities of anti-IgE antibodies produced by neonatal mice fall in the range of values obtained with KLH-IgE in adult mice. Tolerance to unconjugated IgE in CFA can be induced in neonatal mice by administration of IgE in saline. In addition, the tolerant state can be induced by adoptive transfer of spleen cells from adult mice. The time-dependent acquisition of tolerance provides a useful model for studying mechanisms of tolerance and autoimmunity.

Tolerance of Skin Homografts Induced in Adult Mice by Multiple Injections of Homologous Spleen Cells.

1961 ◽  
Vol 106 (3) ◽  
pp. 472-475 ◽  
Author(s):  
F. Shapiro ◽  
C. Martinez ◽  
J. M. Smith ◽  
R. A. Good
Keyword(s):  
Spleen Cells ◽  
Multiple Injections ◽  
Adult Mice

scholarly journals Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response.

1978 ◽  
Vol 147 (3) ◽  
pp. 814-829 ◽  
Author(s):  
D G Sieckmann ◽  
R Asofsky ◽  
D E Mosier ◽  
I M Zitron ◽  
W E Paul
Keyword(s):  
Young Adult ◽  
Cell Cultures ◽  
Mouse Strain ◽  
Proliferative Response ◽  
Tritiated Thymidine ◽  
Spleen Cells ◽  
Adult Mice ◽  
Endotoxin Contamination ◽  
Kinetics Of ◽  
Mouse Lymphocytes

Spleen cell cultures from young adult mice of a variety of strains were stimulated to incorporate tritiated thymidine ([3H]TdR) by a goat anti-mouse IgM antiserum and by purified anti-mu antibodies prepared from this serum. This stimulation was shown to depend upon the anti-mu activity of the antiserum. In addition, ultracentrifuged anti-mu and F(ab')2 fragments of anti-mu were shown to be stimulatory. The anti-mu preparation lacked detectable endotoxin contamination and was also shown to stimulate response by two strains (C57BL/10ScCr and C3H/HeJ) which are unresponsive to the mitogenic effects of endotoxin, while it failed to stimulate a response by cells from a mouse strain (CBA/N) which responds to endotoxin. In addition purified goat anti-mouse gamma, kappa antibodies and rabbit anti-mouse kappa-antib odies stimulated uptake of [3H]TdR by mouse spleen cells, although to a lesser degree than the anti-mu preparation. The cell density, culture requirements, and kinetics of the response are presented.

scholarly journals Newborn Mice Vaccination with BCG.HIVA222+ MVA.HIVA Enhances HIV-1-Specific Immune Responses: Influence of Age and Immunization Routes

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Narcís Saubi ◽  
Eung-Jun Im ◽  
Raquel Fernández-Lloris ◽  
Olga Gil ◽  
Pere-Joan Cardona ◽  
...  
Keyword(s):  
Immune Responses ◽  
The Body ◽  
Spleen Cells ◽  
Vaccination Regimen ◽  
Newborn Mice ◽  
Cellular Immune Responses ◽  
Adult Mice ◽  
Ifn Γ ◽  
Cellular Immune ◽  
Hiv 1

We have evaluated the influence of age and immunization routes for induction of HIV-1- andM. tuberculosis-specific immune responses after neonatal (7 days old) and adult (7 weeks old) BALB/c mice immunization with BCG.HIVA222prime and MVA.HIVA boost. The specific HIV-1 cellular immune responses were analyzed in spleen cells. The body weight of the newborn mice was weekly recorded. The frequencies of HIV-specific CD8+T cells producing IFN-γ were higher in adult mice vaccinated intradermally and lower in adult and newborn mice vaccinated subcutaneously. In all cases the IFN-γ production was significantly higher when mice were primed with BCG.HIVA222compared with BCGwt. When the HIV-specific CTL activity was assessed, the frequencies of specific killing were higher in newborn mice than in adults. The prime-boost vaccination regimen which includes BCG.HIVA222and MVA.HIVA was safe when inoculated to newborn mice. The administration of BCG.HIVA222to newborn mice is safe and immunogenic and increased the HIV-specific responses induced by MVA.HIVA vaccine. It might be a good model for infant HIV and Tuberculosis bivalent vaccine.

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