scholarly journals Nontargeted Stressful Effects in Normal Human Fibroblast Cultures Exposed to Low Fluences of High Charge, High Energy (HZE) Particles: Kinetics of Biologic Responses and Significance of Secondary Radiations

2013 ◽  
Vol 179 (4) ◽  
pp. 444 ◽  
Author(s):  
Géraldine Gonon ◽  
Jean-Emmanuel Groetz ◽  
Sonia M. de Toledo ◽  
Roger W. Howell ◽  
Michel Fromm ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
High Energy ◽  
Normal Human Fibroblast ◽  
High Charge ◽  
Hze Particles ◽  
Human Fibroblast Cultures ◽  
Fibroblast Cultures ◽  
Normal Human ◽  
Kinetics Of

scholarly journals Secretion of β-N-acetylglucosaminidase isoenzymes by normal human fibroblasts

1978 ◽  
Vol 173 (2) ◽  
pp. 433-439 ◽  
Author(s):  
P Willcox
Keyword(s):  
Lysosomal Enzyme ◽  
Human Fibroblast ◽  
Lysosomal Enzymes ◽  
Culture Medium ◽  
Human Fibroblasts ◽  
The Other ◽  
Normal Human Fibroblast ◽  
Acid Hydrolases ◽  
Human Fibroblast Cultures ◽  
Normal Human

1. Secretion of the lysosomal enzyme beta-N-acetylglucosaminidase (EC 3.2.1.30) by normal human fibroblast cultures was linear with respect to time up to 96h. 2. Two forms of the A isoenzyme of beta-N-acetylglucosaminidase were found in the culture medium. One form was similar to the isoenzyme found in other extracellular fluids, such as plasma and tears, the other resembled the intracellular (lysosomal) enzyme. The presence of the two isoenzymes in the culture medium appears to reflect two distinct secretory processes. 3. It is suggested that plasma acid hydrolases may be destined for incorporation into lysosomes in a manner analogous to that described for the packaging of lysosomal enzymes by fibroblasts.

scholarly journals Phosphoprotein profiles of candidate markers for early cellular responses to low-dose γ-radiation in normal human fibroblast cells

2017 ◽  
Vol 58 (3) ◽  
pp. 329-340 ◽  
Author(s):  
Ji-Hye Yim ◽  
Jung Mi Yun ◽  
Ji Young Kim ◽  
In Kyung Lee ◽  
Seon Young Nam ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Human Fibroblast ◽  
Low Dose ◽  
High Dose ◽  
Normal Human Fibroblast ◽  
Low Dose Radiation ◽  
Γ Radiation ◽  
Normal Human ◽  
Dose Radiation ◽  
In Cells

Abstract Ionizing radiation causes biological damage that leads to severe health effects. However, the effects and subsequent health implications caused by exposure to low-dose radiation are unclear. The objective of this study was to determine phosphoprotein profiles in normal human fibroblast cell lines in response to low-dose and high-dose γ-radiation. We examined the cellular response in MRC-5 cells 0.5 h after exposure to 0.05 or 2 Gy. Using 1318 antibodies by antibody array, we observed ≥1.3-fold increases in a number of identified phosphoproteins in cells subjected to low-dose (0.05 Gy) and high-dose (2 Gy) radiation, suggesting that both radiation levels stimulate distinct signaling pathways. Low-dose radiation induced nucleic acid–binding transcription factor activity, developmental processes, and multicellular organismal processes. By contrast, high-dose radiation stimulated apoptotic processes, cell adhesion and regulation, and cellular organization and biogenesis. We found that phospho-BTK (Tyr550) and phospho-Gab2 (Tyr643) protein levels at 0.5 h after treatment were higher in cells subjected to low-dose radiation than in cells treated with high-dose radiation. We also determined that the phosphorylation of BTK and Gab2 in response to ionizing radiation was regulated in a dose-dependent manner in MRC-5 and NHDF cells. Our study provides new insights into the biological responses to low-dose γ-radiation and identifies potential candidate markers for monitoring exposure to low-dose ionizing radiation.

Comparison of Aldolase Isozymes in Placenta, HeLa Cells, and Human Fibroblast Cultures

Enzyme ◽  
1977 ◽  
Vol 22 (6) ◽  
pp. 416-419 ◽  
Author(s):  
Rebecca Gliksman ◽  
Nimai K. Ghosh ◽  
Rody P. Cox
Keyword(s):  
Hela Cells ◽  
Human Fibroblast ◽  
Human Fibroblast Cultures ◽  
Fibroblast Cultures
Sign in / Sign up

Export Citation Format

Share Document