scholarly journals Homologous Recombination Contributes to the Repair of DNA Double-Strand Breaks Induced by High-Energy Iron Ions

2010 ◽  
Vol 173 (1) ◽  
pp. 27 ◽  
Author(s):  
Faria Zafar ◽  
Sara B. Seidler ◽  
Amy Kronenberg ◽  
David Schild ◽  
Claudia Wiese
Keyword(s):  
Homologous Recombination ◽  
High Energy ◽  
Double Strand Breaks ◽  
Iron Ions ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

DNA Double-Strand Breaks Induced by High-Energy Neon and Iron Ions in Human Fibroblasts. II. Probing Individual NotI Fragments by Hybridization

1994 ◽  
Vol 139 (2) ◽  
pp. 142 ◽  
Author(s):  
Markus Löbrich ◽  
Björn Rydberg ◽  
Priscilla K. Cooper ◽  
Markus Lobrich ◽  
Bjorn Rydberg
Keyword(s):  
Human Fibroblasts ◽  
High Energy ◽  
Double Strand Breaks ◽  
Iron Ions ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

scholarly journals End-resection at DNA double-strand breaks in the three domains of life

2013 ◽  
Vol 41 (1) ◽  
pp. 314-320 ◽  
Author(s):  
John K. Blackwood ◽  
Neil J. Rzechorzek ◽  
Sian M. Bray ◽  
Joseph D. Maman ◽  
Luca Pellegrini ◽  
...  
Keyword(s):  
Dna Repair ◽  
Homologous Recombination ◽  
Strand Break ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
Homologous Chromosomes ◽  
Domains Of Life ◽  
Strand Invasion ◽  
End Resection

During DNA repair by HR (homologous recombination), the ends of a DNA DSB (double-strand break) must be resected to generate single-stranded tails, which are required for strand invasion and exchange with homologous chromosomes. This 5′–3′ end-resection of the DNA duplex is an essential process, conserved across all three domains of life: the bacteria, eukaryota and archaea. In the present review, we examine the numerous and redundant helicase and nuclease systems that function as the enzymatic analogues for this crucial process in the three major phylogenetic divisions.

scholarly journals CTCF cooperates with CtIP to drive homologous recombination repair of double-strand breaks

2019 ◽  
Vol 47 (17) ◽  
pp. 9160-9179 ◽  
Author(s):  
Soon Young Hwang ◽  
Mi Ae Kang ◽  
Chul Joon Baik ◽  
Yejin Lee ◽  
Ngo Thanh Hang ◽  
...  
Keyword(s):  
Homologous Recombination ◽  
Critical Role ◽  
Control Point ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks ◽  
C Terminus ◽  
Dna End Resection ◽  
End Resection

Abstract The pleiotropic CCCTC-binding factor (CTCF) plays a role in homologous recombination (HR) repair of DNA double-strand breaks (DSBs). However, the precise mechanistic role of CTCF in HR remains largely unclear. Here, we show that CTCF engages in DNA end resection, which is the initial, crucial step in HR, through its interactions with MRE11 and CtIP. Depletion of CTCF profoundly impairs HR and attenuates CtIP recruitment at DSBs. CTCF physically interacts with MRE11 and CtIP and promotes CtIP recruitment to sites of DNA damage. Subsequently, CTCF facilitates DNA end resection to allow HR, in conjunction with MRE11–CtIP. Notably, the zinc finger domain of CTCF binds to both MRE11 and CtIP and enables proficient CtIP recruitment, DNA end resection and HR. The N-terminus of CTCF is able to bind to only MRE11 and its C-terminus is incapable of binding to MRE11 and CtIP, thereby resulting in compromised CtIP recruitment, DSB resection and HR. Overall, this suggests an important function of CTCF in DNA end resection through the recruitment of CtIP at DSBs. Collectively, our findings identify a critical role of CTCF at the first control point in selecting the HR repair pathway.

scholarly journals Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination

Nature ◽  
10.1038/43932 ◽  
1999 ◽  
Vol 401 (6751) ◽  
pp. 397-399 ◽  
Author(s):  
Roger D. Johnson ◽  
Nan Liu ◽  
Maria Jasin
Keyword(s):  
Homologous Recombination ◽  
Double Strand Breaks ◽  
Dna Double Strand Breaks ◽  
Strand Breaks
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