Gamma-Tocotrienol Protects Hematopoietic Stem and Progenitor Cells in Mice after Total-Body Irradiation

2010 ◽  
Vol 173 (6) ◽  
pp. 738-747 ◽  
Author(s):  
Shilpa Kulkarni ◽  
Sanchita P. Ghosh ◽  
Merriline Satyamitra ◽  
Steven Mog ◽  
Kevin Hieber ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Total Body Irradiation ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
Total Body ◽  
Gamma Tocotrienol

Low-dose total body irradiation causes clonal fluctuation of primate hematopoietic stem and progenitor cells

Blood ◽  
2005 ◽  
Vol 105 (3) ◽  
pp. 1010-1015 ◽  
Author(s):  
Mikko O. Laukkanen ◽  
Ken Kuramoto ◽  
Boris Calmels ◽  
Masaaki Takatoku ◽  
Christof von Kalle ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Progenitor Cells ◽  
Total Body Irradiation ◽  
Low Dose ◽  
Cytotoxic Agents ◽  
High Dose ◽  
Hematopoietic Stem ◽  
Dose Irradiation ◽  
Total Body

Abstract Due to high frequency of side effects caused by high-dose total body irradiation (TBI) the nonmyeloablative regimen together with cytotoxic agents is currently used especially for elderly patients. However, immediate and long-term effects of low-dose irradiation used in allogeneic transplantation on stem cells is less well known. We have studied the effect of low-dose 3 Gy TBI on the number of hematopoietic stem cell (HSC) clones contributing simultaneously to granulocyte production in rhesus macaque. The number of clones after 3 Gy TBI decreased markedly by 2 to 3 weeks after 3 Gy TBI, followed by a period of clonal instability, and recovery to almost pre–3 Gy TBI clonal diversity. The clones accounting for this recovery contributed before 3 Gy TBI, suggesting the profound initial impact of TBI was on a pool of progenitor cells, whereas most of the more primitive HSCs remained unaffected and were able to again contribute to hematopoiesis after recovery. Clonal fluctuation may indirectly suggest the presence of short-term/long-term HSC populations in rhesus macaque bone marrow as reported in a mouse model. The results indicate that even low-dose irradiation affects hematopoietic clonal dynamics and have implications for design of conditioning regimens for transplantation purposes.

Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells

2001 ◽  
Vol 38 (2) ◽  
pp. 139-147
Author(s):  
Jan W. Gratama ◽  
D. Robert Sutherland ◽  
Michael Keeney
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Flow Cytometric ◽  
Stem And Progenitor Cells

Enhanced thrombin/PAR1 activity promotes G-CSF- and AMD3100-induced mobilization of hematopoietic stem and progenitor cells via NO upregulation

Leukemia ◽  
2021 ◽  
Author(s):  
Neta Nevo ◽  
Lizeth-Alejandra Ordonez-Moreno ◽  
Shiri Gur-Cohen ◽  
Francesca Avemaria ◽  
Suditi Bhattacharya ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

scholarly journals Total Body Irradiation for Hematopoietic Stem Cell Transplantation: What Can We Agree on?

2021 ◽  
Vol 28 (1) ◽  
pp. 903-917
Author(s):  
Mitchell Sabloff ◽  
Steven Tisseverasinghe ◽  
Mustafa Ege Babadagli ◽  
Rajiv Samant
Keyword(s):  
Cell Transplantation ◽  
Total Body Irradiation ◽  
Hematopoietic Cell Transplantation ◽  
Conditioning Regimen ◽  
Late Toxicity ◽  
Hepatic Function ◽  
Vascular Supply ◽  
Entire Body ◽  
Hematopoietic Stem ◽  
Total Body

Total body irradiation (TBI), used as part of the conditioning regimen prior to allogeneic and autologous hematopoietic cell transplantation, is the delivery of a relatively homogeneous dose of radiation to the entire body. TBI has a dual role, being cytotoxic and immunosuppressive. This allows it to eliminate disease and create “space” in the marrow while also impairing the immune system from rejecting the foreign donor cells being transplanted. Advantages that TBI may have over chemotherapy alone are that it may achieve greater tumour cytotoxicity and better tissue penetration than chemotherapy as its delivery is independent of vascular supply and physiologic barriers such as renal and hepatic function. Therefore, the so-called “sanctuary” sites such as the central nervous system (CNS), testes, and orbits or other sites with limited blood supply are not off-limits to radiation. Nevertheless, TBI is hampered by challenging logistics of administration, coordination between hematology and radiation oncology departments, increased rates of acute treatment-related morbidity and mortality along with late toxicity to other tissues. Newer technologies and a better understanding of the biology and physics of TBI has allowed the field to develop novel delivery systems which may help to deliver radiation more safely while maintaining its efficacy. However, continued research and collaboration are needed to determine the best approaches for the use of TBI in the future.

3026 – METABOLIC ADAPTATION IN HEMATOPOIETIC STEM AND PROGENITOR CELLS DURING EMERGENCY MYELOPOIESIS

2020 ◽  
Vol 88 ◽  
pp. S46
Author(s):  
Oakley Olson ◽  
Fernando Calero-Nieto ◽  
Xiaonan Wang ◽  
Bethold Göttgens ◽  
Emmanuelle Passegué
Keyword(s):  
Progenitor Cells ◽  
Metabolic Adaptation ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells ◽  
Emergency Myelopoiesis
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