Distribution of Double-Strand Breaks Induced by Ionizing Radiation at the Level of Single DNA Molecules Examined by Atomic Force Microscopy

2009 ◽  
Vol 172 (3) ◽  
pp. 288-295 ◽  
Author(s):  
K. Psonka-Antonczyk ◽  
Th Elsässer ◽  
E. Gudowska-Nowak ◽  
G. Taucher-Scholz
Keyword(s):  
Atomic Force Microscopy ◽  
Ionizing Radiation ◽  
Double Strand Breaks ◽  
Dna Molecules ◽  
Strand Breaks ◽  
Force Microscopy ◽  
Single Dna Molecules ◽  
Atomic Force

scholarly journals Detecting Ultraviolet Damage in Single DNA Molecules by Atomic Force Microscopy

2007 ◽  
Vol 93 (5) ◽  
pp. 1758-1767 ◽  
Author(s):  
Yong Jiang ◽  
Changhong Ke ◽  
Piotr A. Mieczkowski ◽  
Piotr E. Marszalek
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Single Dna Molecules ◽  
Atomic Force ◽  
Ultraviolet Damage

Spatial Distribution of Radiation-Induced Double-Strand Breaks in Plasmid DNA as Resolved by Atomic Force Microscopy

2005 ◽  
Vol 164 (6) ◽  
pp. 755-765 ◽  
Author(s):  
Dalong Pang ◽  
James E. Rodgers ◽  
Barry L. Berman ◽  
Sergey Chasovskikh ◽  
Anatoly Dritschilo
Keyword(s):  
Atomic Force Microscopy ◽  
Spatial Distribution ◽  
Plasmid Dna ◽  
Double Strand Breaks ◽  
Strand Breaks ◽  
Force Microscopy ◽  
Atomic Force ◽  
Radiation Induced

Unusual DNA Structures Formed on Bare Highly Oriented Pyrolytic Graphite Surfaces Studied by Atomic Force Microscopy

2013 ◽  
Vol 19 (3) ◽  
pp. 544-552 ◽  
Author(s):  
Zhiguo Liu ◽  
Lin Zhao ◽  
Yuangang Zu ◽  
Shengnan Tan ◽  
Yuanlin Wang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Structural Characteristics ◽  
Pyrolytic Graphite ◽  
Dna Molecules ◽  
Highly Oriented Pyrolytic Graphite ◽  
Dna Structures ◽  
Force Microscopy ◽  
Single Dna Molecules ◽  
Research Areas ◽  
Atomic Force

AbstractIt is important to know the detailed DNA structure on carbonaceous surfaces for further application of DNA-functionalized carbonaceous materials in diverse research areas. In this study, the topographic and structural characteristics of the separated single DNA molecules and their assembly on highly oriented pyrolytic graphite (HOPG) surfaces have been investigated by atomic force microscopy (AFM). AFM results indicate that both circular and linear DNA molecules tend to form hexagonal patterns along with some unusual structures that include node, protrusion, cruciform, parallel single-stranded DNA (ssDNA), and compact zigzag. Furthermore, parallel ssDNA patterns and their crossed structures have been obtained under high-temperature conditions. Our AFM results reveal that a bare HOPG surface can induce DNA molecules to form various unusual structures. This finding is helpful for understanding the adsorption behavior of DNA on other carbonaceous surfaces such as carbon nanotubes and graphene. In addition, the hexagonal DNA patterns in this study are similar to those formed on the alkylamine-modified HOPG surface, which implies that a bare HOPG, without any chemical modification, has a strong ability to align biomolecules. This study could expand our knowledge of the diversities of DNA structures and the aligning ability of carbonaceous surfaces.

Thermoplastic Nanofluidic Devices for Identifying Abasic Sites in Single DNA Molecules

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Steven A Soper ◽  
Swarnagowri Vaidyanathan ◽  
Franklin Uba ◽  
Bo Hu ◽  
David Kaufman ◽  
...  
Keyword(s):  
Dna Damage ◽  
Therapeutic Efficacy ◽  
Double Strand Breaks ◽  
Dna Molecules ◽  
Strand Breaks ◽  
Single Dna Molecules ◽  
Abasic Sites ◽  
Nanofluidic Devices ◽  
Ap Sites

DNA damage can take many forms such as double-strand breaks and/or the formation of abasic (apurinic/apyrimidinic; AP) sites. The presence of AP sites can be used to determine therapeutic efficacy...

scholarly journals Atomic force microscopy of DNA molecules

FEBS Letters ◽  
1992 ◽  
Vol 301 (2) ◽  
pp. 173-176 ◽  
Author(s):  
Jie Yang ◽  
Kunio Takeyasu ◽  
Zhifeng Shao
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Molecules ◽  
Force Microscopy ◽  
Atomic Force
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