The CGL1 (HeLa × Normal Skin Fibroblast) Human Hybrid Cell Line: A History of Ionizing Radiation Induced Effects on Neoplastic Transformation and Novel Future Directions in SNOLAB

2017 ◽  
Vol 188 (4.2) ◽  
pp. 512-524 ◽  
Author(s):  
Jake S. Pirkkanen ◽  
Douglas R. Boreham ◽  
Marc S. Mendonca
Keyword(s):  
Ionizing Radiation ◽  
Cell Line ◽  
Skin Fibroblast ◽  
Hybrid Cell ◽  
Normal Skin ◽  
Neoplastic Transformation ◽  
Future Directions ◽  
History Of ◽  
Radiation Induced ◽  
Normal Skin Fibroblast

Overexpression of miR-340-5p Inhibits Skin Fibroblast Proliferation by Targeting Kruppel-like Factor 2

2019 ◽  
Vol 20 (13) ◽  
pp. 1147-1154 ◽  
Author(s):  
Ling Chen ◽  
Qian Li ◽  
Xun Lu ◽  
Xiaohua Dong ◽  
Jingyun Li
Keyword(s):  
Gene Ontology ◽  
Skin Fibroblast ◽  
Kegg Pathway ◽  
Normal Skin ◽  
Skin Fibroblasts ◽  
Differentially Expressed ◽  
Luciferase Reporter ◽  
Fibroblast Proliferation ◽  
Pathway Analyses ◽  
Normal Skin Fibroblast

<P>Objective: MicroRNA (miR)-340-5p has been identified to play a key role in several cancers. However, the function of miR-340-5p in skin fibroblasts remains largely unknown. </P><P> Methods: Gain of function experiments were performed by infecting normal skin fibroblast cells with a lentivirus carrying 22-bp miR-340-5p. Cell proliferation was detected by Cell Counting Kit-8 (CCK-8) assay. To uncover the mechanisms, mRNA-seq was used. Differentially expressed mRNAs were further determined by Gene Ontology and KEGG pathway analyses. The protein levels were analysed by Western blotting. A dual-luciferase reporter assay was used to detect the direct binding of miR-340-5p with the 3&#039;UTR of Kruppel-like factor 2 (KLF2). </P><P> Results: MiR-340-5p lentivirus infection suppressed normal skin fibroblast proliferation. The mRNAseq data revealed that 41 mRNAs were differentially expressed, including 22 upregulated and 19 downregulated transcripts in the miR-340-5p overexpression group compared with those in the control group. Gene Ontology and KEGG pathway analyses revealed that miR-340-5p overexpression correlated with the macromolecule biosynthetic process, cellular macromolecule biosynthetic process, membrane, and MAPK signalling pathway. Bioinformatics analysis and luciferase reporter assays showed that miR-340-5p binds to the 3&#039;UTR of KLF2. Forced expression of miR-340-5p decreased the expression of KLF2 in normal skin fibroblasts. Overexpression of KLF2 restored skin fibroblast proliferation in the miR-340-5p overexpression group. </P><P> Conclusion: This study demonstrates that miR-340-5p may suppress skin fibroblast proliferation, possibly through targeting KLF2. These findings could help us understand the function of miR-340-5p in skin fibroblasts. miR-340-5p could be a therapeutic target for preventing scarring.</P>

ROLE OF PHENERGAN IN ABNORMAL SCARS AND KELOIDS

2004 ◽  
Vol 12 (04) ◽  
pp. 471-482
Author(s):  
J. VENUGOPAL ◽  
V. JAYARAMAN ◽  
MARY BABU ◽  
S. RAMAKRISHNA
Keyword(s):  
Dna Synthesis ◽  
Hypertrophic Scar ◽  
Skin Fibroblast ◽  
Collagen Synthesis ◽  
Normal Skin ◽  
Compression Therapy ◽  
Matrix Proteins ◽  
Intralesional Corticosteroid ◽  
Normal Skin Fibroblast

Hypertrophic scar and keloids have affected patients and frustrated physicians for centuries. Hypertrophic scar (HSc) and keloids are a major problem for patients who survive extensive thermal and traumatic skin injuries. HSc and other fibroproliferative disorders are associated with excessive accumulation of collagen and extracellular matrix proteins due to an imbalance between synthesis and degradation. The therapeutic management of hypertrophic scars and keloids include occlusive dressings, compression therapy, intralesional corticosteroid injections, cryosurgery, excision, radiation therapy, laser therapy, interferon therapy and other promising lesser known therapies directed at collagen synthesis. In this study we investigated the effect of phenergan (promethazine hydrochloride) as one of the most potent histamine antagonists on cell proliferation, DNA synthesis and collagen production in fibroblast isolated from human post burn hypertrophic scar, keloids and normal skin. The proliferation of normal skin fibroblast was slightly decreased but hypertrophic scar and keloids showed significant (p<0.001) level of decrease after 72 hours of phenergan (750 μM) treatment. The results of DNA synthesis also significantly (p<0.001) decreased in hypertrophic scar and keloid fibroblasts. Phenergan (1.5 mM) decreased the collagen synthesis upto 61% and 66% in HSc and keloids in comparison to normal skin fibroblast, which showed reduction of 38% after 72 hours. Improved understanding of such regulatory mechanisms may eventually be of therapeutic significance in the control of hypertrophic scar and keloids.

A Simplified and Rapid Staining Method for the HeLa × Skin Fibroblast Human Hybrid Cell Neoplastic Transformation Assay

1992 ◽  
Vol 131 (3) ◽  
pp. 345 ◽  
Author(s):  
Marc S. Mendonca ◽  
Ronald J. Antoniono ◽  
Ch'i Sun ◽  
J. Leslie Redpath
Keyword(s):  
Skin Fibroblast ◽  
Staining Method ◽  
Hybrid Cell ◽  
Neoplastic Transformation ◽  
Transformation Assay

Loss of Suppressor Loci on Chromosomes 11 and 14 May Be Required for Radiation-Induced Neoplastic Transformation of HeLa × Skin Fibroblast Human Cell Hybrids

1998 ◽  
Vol 149 (3) ◽  
pp. 246 ◽  
Author(s):  
Marc S. Mendonca ◽  
Kelly Howard ◽  
Clare L. Fasching ◽  
Daphne L. Farrington ◽  
Lael A. Desmond ◽  
...  
Keyword(s):  
Human Cell ◽  
Skin Fibroblast ◽  
Neoplastic Transformation ◽  
Cell Hybrids ◽  
Radiation Induced
Sign in / Sign up

Export Citation Format

Share Document