Linear Energy Transfer Modulates Radiation-Induced NF-kappa B Activation and Expression of its Downstream Target Genes

2018 ◽  
Vol 189 (4) ◽  
pp. 354-370 ◽  
Author(s):  
Arif Ali Chishti ◽  
Christa Baumstark-Khan ◽  
Kristina Koch ◽  
Waldemar Kolanus ◽  
Sebastian Feles ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Linear Energy Transfer ◽  
Target Genes ◽  
Nf Kappa B ◽  
Downstream Target ◽  
Radiation Induced

scholarly journals Icariin Intervenes in Cardiac Inflammaging through Upregulation of SIRT6 Enzyme Activity and Inhibition of the NF-Kappa B Pathway

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Yang Chen ◽  
Tao Sun ◽  
Junzhen Wu ◽  
Bill Kalionis ◽  
Changcheng Zhang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Enzymatic Activity ◽  
Histone Deacetylase ◽  
Target Genes ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Cell Models ◽  
Animal Tissues ◽  
Nf Kappa B ◽  
Downstream Target

The aim of the study was to investigate the effect of icariin (ICA) on cardiac aging through its effects on the SIRT6 enzyme and on the NF-κB pathway. Investigating the effect of ICA on the enzymatic activity of histone deacetylase SIRT6 revealed a concentration of 10−8 mol/L ICA had a maximum activating effect on histone deacetylase SIRT6 enzymatic activity. Western analysis showed that ICA upregulated SIRT6 protein expression and downregulated NF-κB (p65) protein expression in animal tissues and cell models. ICA upregulated the expression of SIRT6 and had an inhibitory effect on NF-κB inflammatory signaling pathways as shown by decreasing mRNA levels of the NF-κB downstream target genes TNF-α, ICAM-1, IL-2, and IL-6. Those effects were mediated directly or indirectly by SIRT6. We provided evidence that inflammaging may involve a novel link between the effects of ICA on SIRT6 (a regulator of aging) and NF-κB (a regulator of inflammation).

scholarly journals Role of autophagy in high linear energy transfer radiation‐induced cytotoxicity to tumor cells

2014 ◽  
Vol 105 (7) ◽  
pp. 770-778 ◽  
Author(s):  
Xiaodong Jin ◽  
Yan Liu ◽  
Fei Ye ◽  
Xiongxiong Liu ◽  
Yoshiya Furusawa ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Tumor Cells ◽  
Linear Energy Transfer ◽  
Radiation Induced ◽  
Linear Energy Transfer Radiation ◽  
High Linear Energy Transfer

scholarly journals Distinct Roles of Ape1 Protein, an Enzyme Involved in DNA Repair, in High or Low Linear Energy Transfer Ionizing Radiation-induced Cell Killing

2014 ◽  
Vol 289 (44) ◽  
pp. 30635-30644 ◽  
Author(s):  
Hongyan Wang ◽  
Xiang Wang ◽  
Guangnan Chen ◽  
Xiangming Zhang ◽  
Xiaobing Tang ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Linear Energy Transfer ◽  
Cell Killing ◽  
Dna Fragments ◽  
End Joining ◽  
Heavy Charged Particles ◽  
High Let Radiation ◽  
High Let ◽  
Radiation Induced ◽  
Non Homologous End Joining

High linear energy transfer (LET) radiation from space heavy charged particles or a heavier ion radiotherapy machine kills more cells than low LET radiation, mainly because high LET radiation-induced DNA damage is more difficult to repair. Relative biological effectiveness (RBE) is the ratio of the effects generated by high LET radiation to low LET radiation. Previously, our group and others demonstrated that the cell-killing RBE is involved in the interference of high LET radiation with non-homologous end joining but not homologous recombination repair. This effect is attributable, in part, to the small DNA fragments (≤40 bp) directly produced by high LET radiation, the size of which prevents Ku protein from efficiently binding to the two ends of one fragment at the same time, thereby reducing non-homologous end joining efficiency. Here we demonstrate that Ape1, an enzyme required for processing apurinic/apyrimidinic (known as abasic) sites, is also involved in the generation of small DNA fragments during the repair of high LET radiation-induced base damage, which contributes to the higher RBE of high LET radiation-induced cell killing. This discovery opens a new direction to develop approaches for either protecting astronauts from exposure to space radiation or benefiting cancer patients by sensitizing tumor cells to high LET radiotherapy.

scholarly journals Withdrawal: Distinct roles of Ape1 protein, an enzyme involved in DNA repair, in high or low linear energy transfer ionizing radiation-induced cell killing.

2020 ◽  
Vol 295 (18) ◽  
pp. 6249-6249
Author(s):  
Hongyan Wang ◽  
Xiang Wang ◽  
Guangnan Chen ◽  
Xiangming Zhang ◽  
Xiaobing Tang ◽  
...  
Keyword(s):  
Dna Repair ◽  
Energy Transfer ◽  
Ionizing Radiation ◽  
Linear Energy Transfer ◽  
Cell Killing ◽  
Radiation Induced
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