New Insight into Quantitative Modeling of DNA Double-Strand Break Rejoining

2015 ◽  
Vol 184 (3) ◽  
pp. 280 ◽  
Author(s):  
Lisa Herr ◽  
Igor Shuryak ◽  
Thomas Friedrich ◽  
Michael Scholz ◽  
Marco Durante ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Quantitative Modeling ◽  
Dna Double Strand Break ◽  
Insight Into

scholarly journals Modulation of Saccharomyces cerevisiae DNA double-strand break repair by SRS2 and RAD51.

Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1189-1199
Author(s):  
G T Milne ◽  
T Ho ◽  
D T Weaver
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Recombinational Repair ◽  
Multiple Alleles ◽  
Dna Double Strand Break ◽  
Repair Mechanisms ◽  
Break Repair ◽  
Insight Into

Abstract RAD52 function is required for virtually all DNA double-strand break repair and recombination events in Saccharomyces cerevisiae. To gain greater insight into the mechanism of RAD52-mediated repair, we screened for genes that suppress partially active alleles of RAD52 when mutant or overexpressed. Described here is the isolation of a phenotypic null allele of SRS2 that suppressed multiple alleles of RAD52 (rad52B, rad52D, rad52-1 and KlRAD52) and RAD51 (KlRAD51) but failed to suppress either a rad52 delta or a rad51 delta. These results indicate that SRS2 antagonizes RAD51 and RAD52 function in recombinational repair. The mechanism of suppression of RAD52 alleles by srs2 is distinct from that which has been previously described for RAD51 overexpression, as both conditions were shown to act additively with respect to the rad52B allele. Furthermore, overexpression of either RAD52 or RAD51 enhanced the recombination-dependent sensitivity of an srs2 delta RAD52 strain, suggesting that RAD52 and RAD51 positively influence recombinational repair mechanisms. Thus, RAD52-dependent recombinational repair is controlled both negatively and positively.

scholarly journals The dmc1 Mutant Allows an Insight Into the DNA Double-Strand Break Repair During Meiosis in Barley (Hordeum vulgare L.)

2019 ◽  
Vol 10 ◽  
Author(s):  
Miriam Szurman-Zubrzycka ◽  
Brygida Baran ◽  
Magdalena Stolarek-Januszkiewicz ◽  
Jolanta Kwaśniewska ◽  
Iwona Szarejko ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Hordeum Vulgare L ◽  
Dna Double Strand Break ◽  
Break Repair ◽  
Insight Into
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