Mitochondrial DNA Barcoding Reveals First Documented Evidence of Golden Redhorse (Moxostoma erythrurum) in the Susquehanna River, Pennsylvania

2021 ◽  
Vol 28 (2) ◽  
Author(s):  
Gregory R Moyer ◽  
Ryan Shaw ◽  
Timothy Wertz
Keyword(s):  
Mitochondrial Dna ◽  
Dna Barcoding ◽  
Susquehanna River

Mitochondrial DNA Barcoding of the Pacific Oyster Crassostrea gigas (Thunberg, 1793) (Mollusca: Bivalvia: Ostreidae), Cultivated in the Black Sea

2018 ◽  
Vol 54 (12) ◽  
pp. 1445-1451
Author(s):  
Yu. V. Slynko ◽  
E. E. Slynko ◽  
A. V. Pirkova ◽  
L. V. Ladygina ◽  
V. I. Ryabushko
Keyword(s):  
Mitochondrial Dna ◽  
Dna Barcoding ◽  
Black Sea ◽  
Crassostrea Gigas ◽  
Pacific Oyster ◽  
The Black Sea ◽  
The Pacific

scholarly journals Purifying selection can reduce intraspecific mitochondrial gene variation to that of nuclear rRNA

2020 ◽  
Author(s):  
Tshifhiwa G. Matumba ◽  
Jody Oliver ◽  
Nigel P. Barker ◽  
Christopher D. McQuaid ◽  
Peter R. Teske
Keyword(s):  
Mitochondrial Dna ◽  
Dna Barcoding ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Population Expansion ◽  
Purifying Selection ◽  
Species Level ◽  
Molecular Dating ◽  
Coi Gene ◽  
28S Rrna

AbstractMitochondrial DNA (mtDNA) has long been used to date the divergence between species, and to explore the time when species’ effective population sizes changed. The idea that mitochondrial DNA is useful for molecular dating rests on the premise that its evolution is neutral. This premise was questionable to begin with, and even though it has long been challenged, the evidence against clock-like evolution of mtDNA is usually ignored. Here, we present a particularly clear and simple example to illustrate the implications of violations of the assumption of selective neutrality. DNA sequences were generated for the mtDNA COI gene and the nuclear 28S rRNA of two closely related and widely distributed rocky shore snails whose geographical ranges are defined by different thermal preferences. To our knowledge, this is the first study to use nuclear rRNA sequence for studying species-level genealogies instead of phylogenetics, presumably because this marker is considered to be uninformative at this taxonomic level. Even though the COI gene evolves at least an order of magnitude faster, which was reflected in high inter-specific divergence, intraspecific genetic variation was similar for both markers. As a result, estimates of population expansion times based on mismatch distributions were completely different for the two markers. Assuming that 28S evolves effectively clock-like, these findings likely illustrate variation-reducing purifying selection in mtDNA at the species level, and an elevated divergence rate caused by divergent selection between the two species. Although these two selective forces together make mtDNA suitable as a DNA barcoding marker because they create a ‘barcoding gap’, estimates of demographic change can be expected to be highly unreliable. Our study contributes to the growing evidence that the utility of mtDNA beyond DNA barcoding is limited.

Mitochondrial DNA barcoding detects some species that are real, and some that are not

2010 ◽  
Vol 10 (2) ◽  
pp. 264-273 ◽  
Author(s):  
KANCHON K. DASMAHAPATRA ◽  
MARIANNE ELIAS ◽  
RYAN I. HILL ◽  
JOSEPH I. HOFFMAN ◽  
JAMES MALLET
Keyword(s):  
Mitochondrial Dna ◽  
Dna Barcoding

scholarly journals Karakteristik Molekuler Kelelawar (Microchiroptera), berdasarkan DNA Mitokondria (Gen COI) di Gua Sukabumi dan Sentul Jawa Barat

2021 ◽  
Vol 14 (1) ◽  
pp. 20-28
Author(s):  
Ismayati Afifah ◽  
Dedy Duryadi Solihin ◽  
Arzyana Sunkar
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Distance ◽  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Cytochrome Oxidase I ◽  
Genetic Identity ◽  
Genetic Identification ◽  
Coi Gene ◽  
Genetic Characteristics ◽  
Spin Column

AbstrakCytochrome Oxidase I (COI) merupakan salah satu gen mitokondria untuk membantu konstruksi dari pohon filogeni yang dapat bertindak sebagai gen marker. Gen COI memiliki keakuratan dalam mengidentifikasi spesies dan umumnya digunakan sebagai “DNA Barcoding”. Informasi mengenai karakteristik genetik berdasarkan DNA mitokondria pada kelelawar di Sukabumi dan Sentul belum banyak dilaporkan. Tujuan dari penelitian ini untuk mengetahui keragaman genetik kelelawar berdasarkan DNA mitokondria dengan penanda Cytochrome Oxidase I (COI) sebagai DNA barcoding. Isolasi DNA total dilakukan menggunakan Kit Dneasy® Blood and Tissue Kit cat no 69504 (50) berdasarkan prosedur Spin-Column Protocol dengan modifikasi. Hasil penelitian ini menunjukkan bahwa gen COI telah berhasil mengidentifikasi karakteristik spesies. Dua haplotipe didapatkan dari masing-masing populasi. Berdasarkan barcode DNA menunjukkan populasi Sukabumi merupakan spesies Chaerephon plicatus dengan nilai identitas genetik sebesar 97,08%, sedangkan populasi Sentul menunjukkan perbedaan secara genetik dengan spesies Hipposideros larvatus dengan nilai identitas genetik sebesar 94,85%. Identifikasi secara genetik dengan menggunakan gen COI menunjukkan bahwa kelelawar yang berasal Sukabumi adalah spesies Chaerephon plicatus dengan jarak genetik sebesar 3,1%. Kelelawar yang berasal dari Sentul memiliki kedekatan dengan spesies Hipposideros larvatus namun memiliki jarak genetik sebesar 5,2%. AbstractCytochrome Oxidase I (COI) is one of the mitochondrial genes to help the construction of phylogeny trees that can act as marker genes. The COI gene has accuracy in identifying species and is commonly used as "DNA Barcoding". Information about genetic characteristics based on mitochondrial DNA in bats in Sukabumi and Sentul has not been widely reported. The purpose of this study was to determine the genetic diversity of bats based on Mitochondrial DNA with Cytochrome Oxidase I (COI) markers as DNA barcoding. Total DNA isolation was carried out using the Dneasy® Blood and Tissue Kit paint no. 69504 (50) based on the Spin-Column Protocol procedure with modifications. The results of this study indicate that the COI gene has successfully identified species characteristics. Two haplotypes were obtained from each population. Based on DNA barcodes, the population of Sukabumi is a species of Chaerephon plicatus with a genetic identity value of 97.08%, while the Sentul population shows genetic differences with the Hipposideros larvatus species with a genetic identity value of 94.85%. Genetic identification using the COI gene shows that the bats originating from Sukabumi is a spesies Chaerephon plicatus with a genetic distance of 3.1%. The bats originating from Sentul are closely related to the species Hipposideros larvatus but have a genetic distance of 5.2%.

scholarly journals Presence of Notahypsibius pallidoides (Tardigrada: Hypsibiidae) in the fauna of Russia confirmed with the methods of DNA barcoding

2021 ◽  
Vol 66 (3) ◽  
Author(s):  
Denis Tumanov
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Variability ◽  
Dna Barcoding ◽  
Dna Barcode ◽  
Morphological Identification ◽  
Saint Petersburg ◽  
Current State

A brief report is given on the tardigrade species Notahypsibius pallidoides, discovered in a moss cushion in the vicinity of Saint Petersburg. Morphological identification of the species is supported with the analysis of obtained sequences of nuclear and mitochondrial DNA barcode genes (ITS-2 and COI). The current state of knowledge of Russian fauna of Tardigrada is characterised, and all available data on the distribution of Notahypsibius pallidoides and its genetic variability are summarised. The data obtained in this study by methods of DNA barcoding demonstrated that studied specimens of the tardigrada population isolated in Russia belong to the same species (Notahypsibius pallidoides) as the control specimens isolated in Austria though represent another haplotype thus confirming the presence of Notahypsibius pallidoides for the fauna of Russia.

scholarly journals Origin of Invasive Fish Species, Peacock Bass Cichla Species in Lake Telabak Malaysia Revealed by Mitochondrial DNA Barcoding

2020 ◽  
Vol 24 (3) ◽  
pp. 311-322
Author(s):  
Aliyu G. Khaleel ◽  
Syafiq A. M. Nasir ◽  
Norshida Ismail ◽  
Kamarudin Ahmad-Syazni
Keyword(s):  
Mitochondrial Dna ◽  
Dna Barcoding ◽  
Fish Species ◽  
Invasive Fish ◽  
Peacock Bass
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