EFFECTS OF COPPER SULFATE TOXICITY ON CERCARIAE AND METACERCARIAE OF ECHINOSTOMA CAPRONI AND ECHINOSTOMA TRIVOLVIS AND ON THE SURVIVAL OF BIOMPHALARIA GLABRATA SNAILS

2004 ◽  
Vol 90 (6) ◽  
pp. 1332-1337 ◽  
Author(s):  
Aditya Reddy ◽  
Elizabeth L. Ponder ◽  
Bernard Fried
Keyword(s):  
Copper Sulfate ◽  
Biomphalaria Glabrata ◽  
Echinostoma Caproni ◽  
Echinostoma Trivolvis

scholarly journals Molluscicide activity of the "Avelós" plant (Euphorbia tirucalli, L. ) on Biomphalaria glabrata, the mollusc vector of schistosomiasis

1985 ◽  
Vol 80 (4) ◽  
pp. 423-427 ◽  
Author(s):  
Pedro Jurberg ◽  
Januário Bispo Cabral Neto ◽  
Virgínia T. Schall
Keyword(s):  
Aqueous Solution ◽  
Copper Sulfate ◽  
Active Substance ◽  
Simple Procedure ◽  
Biomphalaria Glabrata ◽  
Wide Distribution ◽  
Euphorbia Tirucalli ◽  
Promising Agent

An aqueous solution of the latex of Euphorbia tirucalli collected at sites receiving large amounts of sunlight showed molluscicide action on Biomphalaria glabrata, with LD50 obtained at the concentration of 28,0 ppm and LD90 at the concentration of 85,0 ppm. The toxicity of the product for fish was similar to that of Bayluscide and of copper sulfate used for comparison. However, the wide distribution of the plant, its easy propagation and the simple procedure for extraction of the active substance, which is biodegradable, favor "avelós" as a promising agent in the control of schistosomiasis.

Effects of Echinostoma caproni miracidia dose on the neutral and polar lipids of Biomphalaria glabrata as determined by high-performance thin-layer chromatography

2013 ◽  
Vol 58 (4) ◽  
Author(s):  
Alexandra Hunsberger ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Phosphoric Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
Echinostoma Caproni ◽  
Layer Chromatography ◽  
Chloroform Methanol ◽  
Detection Reagent

AbstractThe effects of a 5 versus 25 miracidia exposure of Echinostoma caproni on the lipid composition of Biomphalaria glabrata was studied using high performance thin layer chromatography (HPTLC)-densitometry. A 50 miracidia dose was not used because such a high level of exposure caused severe snail mortality by 3 weeks post-exposure (PE). Lipids were determined in the digestive-gland gonad complex (DGG) of the exposed snails and in the uninfected matched controls at 2 and 4 weeks PE. Extraction of lipids from DGGs was carried out by the Folch method with chloroform-methanol (2:1), and extracts were analyzed on Analtech HPTLC-HLF pre-adsorbent silica gel plates with measurement of separated bands using a CAMAG Scanner 3. For neutral lipids the mobile phase was petroleum ether-diethyl ether-glacial acetic acid (80:20:1) and the detection reagent was 5% ethanolic phosphoric acid, and for polar lipids chloroform-methanol-deionized water (65:25:4) mobile phase and 10% cupric sulfate in 8% phosphoric acid detection reagent were used. No significant differences in the concentrations of free sterols, free fatty acids, triacylglycerols, phosphatidylcholine, and phosphatidylethanolamine were seen at 2 weeks PE in any of the groups. At 4 weeks PE, the free fatty acid concentration increased significantly in the snails exposed to 25 miracidia compared to that of the 5 miracidia/snail group or the controls. Elevation of the free fatty acid fraction in the high dose snail group suggested that some changes occurred in the lipid metabolism of the snails in that group as a function of miracidia dose.

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