Experimental Exposure of Helisoma trivolvis and Biomphalaria glabrata (Gastropoda) to Ribeiroia ondatrae (Trematoda)

2011 ◽  
Vol 97 (6) ◽  
pp. 1055-1061 ◽  
Author(s):  
Miranda D Redmond ◽  
Richard B Hartson ◽  
Jason T Hoverman ◽  
Christina N De Jesús-Villanueva ◽  
Pieter T. J Johnson
Keyword(s):  
Biomphalaria Glabrata ◽  
Experimental Exposure ◽  
Helisoma Trivolvis

Evidence from two planorbid snails of a complex and dedicated response to digenean (echinostome) infection

Parasitology ◽  
1999 ◽  
Vol 119 (4) ◽  
pp. 395-404 ◽  
Author(s):  
C. M. ADEMA ◽  
L. A. HERTEL ◽  
E. S. LOKER
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biomphalaria Glabrata ◽  
The Other ◽  
Digenetic Trematode ◽  
Snail Species ◽  
Helisoma Trivolvis ◽  
General Ability ◽  
Digenean Species ◽  
Reactive Plasma ◽  
Echinostoma Trivolvis

The planorbid snail Biomphalaria glabrata responded to exposure to either the compatible digenetic trematode Echinostoma paraensei or the incompatible species Echinostoma trivolvis by producing increased amounts of several distinctive plasma polypeptides. These polypeptides characteristically precipitated from plasma when mixed with secreted–excreted products (SEP) of sporocysts or rediae from either digenean species. In contrast, control snails, or snails that had been wounded or infected with bacteria (Serratia marcesens or Staphylococcus epidermidis) showed no obvious plasma alterations and no precipitates formed when their plasma was mixed with SEP. Another planorbid species, Helisoma trivolvis, which displays reverse compatibility for the echinostome species used, also responded to exposure to both echinostomes by increased production of plasma polypeptides that precipitated in the presence of SEP. With some individual variation, these 2 snail species synthesized SEP-reactive plasma polypeptides forming diffuse bands centred at 53, 65, 80–120 and 200 kDa (the latter absent in Helisoma trivolvis). The 53 kDa polypeptides had not been observed before, whereas the others have been noted from B. glabrata. The diffuse 65 kDa band was strongly bound by anti-fibrinogen antibodies, supportive of earlier studies indicating it contains fibrinogen-related domains. The other specified polypeptides were also bound by these antibodies raising the possibility that they too contain fibrinogen domains. The results are suggestive of a general ability of these 2 planorbid snails to detect the presence of echinostomes even if the latter are subsequently incapable of development. The complex response they then mount, one not evoked by other challenges such as wounding or bacterial infection, may represent a dedicated response to a frequently encountered group of pathogenic parasites, the digeneans (echinostomes).

Study of the radiomodifier effect of Pityrocarpa moniliformis extract

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
João Victor Torres de Moraes ◽  
Ricardo Luiz Calazans Luna Filho ◽  
Williams Nascimento de Siqueira ◽  
Hianna Arely Milca Fagundes Silva ◽  
Dewson Rocha Pereira ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Cellular Response ◽  
Biomphalaria Glabrata ◽  
Toxicity Tests ◽  
Antioxidant Compounds ◽  
Blastula Stage ◽  
Natural Substances ◽  
60Co Source ◽  
60Co Gamma ◽  
60Co Gamma Radiation

Ionizing radiation has been applied in several areas of knowledge, among them the study of the radiomodifier activity of natural substances. These substances can modify the cellular response to the damage induced by the radiation. Therefore, this work aimed to evaluate the radiomodifier action of Pityrocarpa moniliformis extract on Biomphalaria glabrata embryos exposed to 60Co gamma radiation. Initially, toxicity tests were performed on the extract against the B. glabrata embryos for the choice of concentration that did not cause death and embryonic malformation. Then, the antioxidant activity of the P. moniliformis extract with flavonoids and phenolic compounds was evaluated by means of the ABTS method. To evaluate the radiomodifier activity of the extract, embryos were selected in the blastula stage and irradiated with 7.5 Gy in a 60Co source (gammacell-Co60). Then, the embryos were exposed for 24 h to the extract of P. moniliformis at a concentration of 250 μg/mL. The results showed that the extract of P. moniliformis presents flavonoids and enzymatic inhibition by ABTS, which demonstrates the presence of antioxidant compounds. However, the tests of the radiomodifier activity did not present radioprotective effect for embryos exposed to ionizing radiation.

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