Multiple Paternity in Ixodes ricinus (Acari: Ixodidae), Assessed by Microsatellite Markers

2008 ◽  
Vol 94 (2) ◽  
pp. 345-347 ◽  
Author(s):  
Gunnar Hasle ◽  
Knut H. Røed ◽  
Hans Petter Leinaas
Keyword(s):  
Microsatellite Markers ◽  
Ixodes Ricinus ◽  
Multiple Paternity

Evidence of multiple paternity in Morelet's Crocodile (Crocodylus moreletii) in Belize, CA, inferred from microsatellite markers

2008 ◽  
Vol 309A (10) ◽  
pp. 643-648 ◽  
Author(s):  
John D. McVay ◽  
David Rodriguez ◽  
Thomas R. Rainwater ◽  
Jennifer A. Dever ◽  
Steven G. Platt ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Multiple Paternity ◽  
Morelet's Crocodile ◽  
Crocodylus Moreletii

Promiscuity and high level of multiple paternity in common wall lizards (Podarcis muralis): data from microsatellite markers

2007 ◽  
Vol 28 (2) ◽  
pp. 301-303 ◽  
Author(s):  
Anne Oppliger ◽  
Loic Degen ◽  
Henry-B. John-Alder ◽  
Caroline Bouteiller-Reuter
Keyword(s):  
Microsatellite Markers ◽  
Mating System ◽  
Observational Data ◽  
Wild Population ◽  
Multiple Paternity ◽  
Paternity Analysis ◽  
Podarcis Muralis ◽  
Common Wall ◽  
The Common ◽  
High Level

AbstractLittle is known about the mating system of the common wall lizard Podarcis muralis. Behavioural and observational data have demonstrated that females frequently mate with multiple males. However, the possible occurrence of multiple paternity has never been investigated. By using microsatellite paternity analysis in a wild population, we document this species indeed mate promiscuously and these matings resulted in multiple paternity in at least 87% of the clutches examined.

Detection of multiple paternity and sperm storage in a captive colony of the central Asian tortoise, Testudo horsfieldii

2006 ◽  
Vol 84 (4) ◽  
pp. 520-526 ◽  
Author(s):  
Emily E. Johnston ◽  
Matthew S. Rand ◽  
Stephan G. Zweifel
Keyword(s):  
Reproductive Success ◽  
Microsatellite Markers ◽  
Sperm Competition ◽  
Genomic Library ◽  
Multiple Paternity ◽  
Female Mate Choice ◽  
Sperm Storage ◽  
Male Reproductive Success ◽  
Critical Measure ◽  
Central Asian

Reproductive success is a critical measure of an organism’s fitness. Determining reproductive success in vertebrates is confounded by the concealed mechanism and timing of fertilization (e.g., sperm competition and storage). To assess the relationship between observed mating behavior and reproductive success in the central Asian tortoise, Testudo horsfieldii Gray, 1844, we determined individual genotypes from a captive colony of adults and their offspring. We constructed a size-selected genomic library from T. horsfieldii and screened for polymorphic microsatellite markers. The screen resulted in identification of two novel microsatellite regions. Cross-species amplification of microsatellite markers using primers developed for the bog turtle, Glyptemys muhlenbergii (Schoepff, 1801), resulted in isolation of three additional polymorphic microsatellites for T. horsfieldii. The five loci, which have between 5 and 17 alleles and observed heterozygosities between 0.44 and 0.90, were used to determine the frequency of multiple paternity in the captive colony. We found evidence for multiple paternity in 27% of the clutches examined, as well as evidence for overwinter sperm storage and variance in adult male reproductive success. These data indicate that ample opportunity exists for sperm competition and female mate choice in T. horsfieldii.

Microsatellite markers reveal multiple paternity and sperm storage in the Mediterranean spur-thighed tortoise, Testudo graeca

2004 ◽  
Vol 82 (1) ◽  
pp. 153-159 ◽  
Author(s):  
S Roques ◽  
C Díaz-Paniagua ◽  
A C Andreu
Keyword(s):  
Microsatellite Markers ◽  
Reproductive Strategy ◽  
Multiple Mating ◽  
Multiple Paternity ◽  
Sperm Storage ◽  
Testudo Graeca ◽  
Multiple Matings ◽  
Second Clutch ◽  
Small Clutch ◽  
Microsatellite Analyses

The spur-thighed tortoise (Testudo graeca) is a terrestrial species in which multiple mating is frequently observed. We assessed the probability of multiple paternity in clutches (n = 15) laid by eight females, including successive clutches. Paternity was determined by microsatellite analyses at three loci. A large number of alleles per locus (n = 15–22) provided high probabilities of detecting multiple paternity, particularly at all loci combined (P = 0.989). Multiple paternity was found in 20% of the clutches in which offspring displayed more than two paternal alleles. However, this frequency may have been underestimated, given the small clutch sizes and the few loci used. Also, T. graeca is able to store sperm from single or multiple matings and can use it to fertilize subsequent clutches of eggs, as indicated by the fact that the second clutch of a captive female was sired by a different male and that clutches of another female were multiply sired by the same males. These results confirm that multiple paternity exists in T. graeca and that sperm storage in this species may be an important reproductive strategy to fertilize multiple clutches per year.

Multiple Paternity in the Blue Crab (Callinectes sapidus) Assessed with Microsatellite Markers

2017 ◽  
Vol 36 (1) ◽  
pp. 273-276 ◽  
Author(s):  
Jessica M. Hill ◽  
Ernest P. Williams ◽  
Brian Masters ◽  
Allen R. Place
Keyword(s):  
Microsatellite Markers ◽  
Blue Crab ◽  
Callinectes Sapidus ◽  
Multiple Paternity

scholarly journals Isolation and characterization of new polymorphic microsatellite markers for the tick Ixodes ricinus (Acari, Ixodidae)

Acarologia ◽  
2012 ◽  
Vol 52 (2) ◽  
pp. 123-128 ◽  
Author(s):  
V. Noel ◽  
E. Léger ◽  
E. Gómez-Díaz ◽  
A.-M. Risterucci ◽  
K.D. McCoy
Keyword(s):  
Microsatellite Markers ◽  
Ixodes Ricinus ◽  
Isolation And Characterization ◽  
Polymorphic Microsatellite

scholarly journals Multiple paternity assessed in the cuttlefish Sepiella japonica (Mollusca, Cephalopoda) using microsatellite markers

ZooKeys ◽  
2019 ◽  
Vol 880 ◽  
pp. 33-42
Author(s):  
Liqin Liu ◽  
Yao Zhang ◽  
Xiaoyu Hu ◽  
Zhenming Lü ◽  
Bingjian Liu ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Sperm Competition ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Multiple Paternity ◽  
Paternal Allele ◽  
Fujian Province ◽  
Behavioral Observations ◽  
Microsatellite Dna Markers ◽  
Sepiella Japonica

Multiple paternity was demonstrated for seven clutches of eggs and 40 offspring sampled from these clutches in the cuttlefish Sepiella japonica from Fujian Shacheng Harbor Cultivation Base (Fujian Province, China), using four microsatellite DNA markers. It was observed that female cuttlefish copulated with different males. In this study, genotyping data suggest that at least three paternal allele genotypes were present in all seven clutches indicating that at least two males were responsible for each brood. Combined with behavioral observations, this study provides evidence for sperm competition and multiple paternity in S. japonica.

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