Isolation and Genotyping of Toxoplasma gondii From Free-Ranging Chickens From Argentina

2003 ◽  
Vol 89 (5) ◽  
pp. 1063-1064 ◽  
Author(s):  
J. P. Dubey ◽  
M. C. Venturini ◽  
L. Venturini ◽  
M. Piscopo ◽  
D. H. Graham ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Free Ranging

scholarly journals Detection of Toxoplasma gondii in a free-ranging giant anteater

2017 ◽  
Vol 47 (8) ◽  
Author(s):  
Thais Oliveira Morgado ◽  
Francielle Cristina Kagueyama ◽  
Janaina Marcela Assunção Rosa ◽  
Melissa Debesa Belizário ◽  
Richard de Campos Pacheco ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Zoonotic Infection ◽  
Chronic Infections ◽  
Chain Reaction ◽  
Positive Serology ◽  
Modified Agglutination Test ◽  
Polymerase Chain ◽  
Free Ranging ◽  
First Time

ABSTRACT: Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan, which establishes acute and chronic infections in birds and mammals, including humans. This note reports, for the first time, the detection and sequencing of DNA from T. gondii in the peripheral blood of a young free range giant anteater (Myrmecophaga tridactyla). For the diagnosis, the following methods were used: polymerase chain reaction (PCR) and positive serology (1:800) by means of the modified agglutination test (MAT). Since this species may be consumed by humans and predated by wild felids, its importance is emphasized as a probable source of zoonotic infection, in addition to its possible participation in the infection enzootic cycle. Although, parasitemia has been confirmed in this specimen, it presented no clinical sign of infection.

Molecular and biological characterization ofToxoplasma gondiiisolates from free-range chickens from Guyana, South America, identified several unique and common parasite genotypes

Parasitology ◽  
2007 ◽  
Vol 134 (11) ◽  
pp. 1559-1565 ◽  
Author(s):  
J. P. DUBEY ◽  
L. APPLEWHAITE ◽  
N. SUNDAR ◽  
G. V. VELMURUGAN ◽  
L. A. BANDINI ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
South America ◽  
Rflp Markers ◽  
Free Range ◽  
Clonal Type ◽  
Modified Agglutination Test ◽  
Pcr Rflp ◽  
Free Ranging ◽  
Common Parasite

SUMMARYThe prevalence ofToxoplasma gondiiin free-ranging chickens (Gallus domesticus) is a good indicator of the prevalence ofT. gondiioocysts in the soil because chickens feed from the ground. The prevalence ofT. gondiiin 76 free-range chickens from Guyana, South America was determined. Antibodies toT. gondiiwere assayed by the modified agglutination test (MAT), and found in 50 (65·8%) of 76 chickens with titres of 1:5 in four, 1:10 in one, 1:20 in five, 1:40 in seven, 1:80 in six, 1:160 in eight, 1:320 in four, 1:640 or higher in 15. Hearts and brains of 26 chickens with titres of <1:5 were pooled in 5 batches and bioassayed in mice. Hearts and brains of 50 chickens with titres of 1:5 or higher were bioassayed in mice.Toxoplasma gondiiwas isolated by bioassay in mice from 35 chickens with MAT titres of 1:20 or higher. All mice inoculated with tissues of 30 infected chickens remained asymptomatic.Toxoplasma gondiiisolates from 35 chickens were genotyped using 11 PCR-RFLP markers including SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, a new SAG2, and Apico. A total of 9 genotypes were identified, with 5 genotypes (nos 1, 4, 5, 6 and 7) unique to Guyana, 2 genotypes (nos 2 and 3) previously identified in chickens from Brazil, 1 genotype (no. 8) previously identified in chickens from Brazil, Costa Rica and Nicaragua, and 1 genotype (no. 9) belonging to the clonal type III lineage that exists globally. Infection with 2 genotypes was found from 1 chicken. This is the first report of genetic characterization ofT. gondiiisolates from any host from Guyana.

Seroprevalence of Toxoplasma gondii in American free-ranging or captive pumas (Felis concolor) and bobcats (Lynx rufus)

2004 ◽  
Vol 120 (1-2) ◽  
pp. 1-9 ◽  
Author(s):  
Yoko Kikuchi ◽  
Bruno B Chomel ◽  
Rickie W Kasten ◽  
Janice S Martenson ◽  
Pamela K Swift ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Lynx Rufus ◽  
Felis Concolor ◽  
Free Ranging

Antibodies to Toxoplasma gondii, Leishmania spp., and Leptospira spp. in Free-Ranging Six-Banded Armadillos (Euphractus sexcinctus) from Northeastern Brazil

2020 ◽  
Vol 56 (2) ◽  
pp. 486
Author(s):  
Werona de Oliveira Barbosa ◽  
Thiago Galvão Coelho ◽  
Talita Otaviano da Costa ◽  
Laís Moraes Paiz ◽  
Felipe Fornazari ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Northeastern Brazil ◽  
Leptospira Spp ◽  
Leishmania Spp ◽  
Euphractus Sexcinctus ◽  
Free Ranging

scholarly journals Ecologic Correlates of Toxoplasma gondii Exposure in Free-ranging Neotropical Mammals

2003 ◽  
Vol 39 (2) ◽  
pp. 456-459 ◽  
Author(s):  
Benoit de Thois ◽  
Magalie Demar ◽  
Christine Aznar ◽  
Bernard Carme
Keyword(s):  
Toxoplasma Gondii ◽  
Neotropical Mammals ◽  
Free Ranging

Seroprevalence of Antibodies Against Toxoplasma gondii in Free-ranging Mammals in Iowa

1998 ◽  
Vol 34 (4) ◽  
pp. 811-815 ◽  
Author(s):  
Richard E. Hill ◽  
Jeff J. Zimmerman ◽  
Robert W. Wills ◽  
Sharon Patton ◽  
William R. Clark
Keyword(s):  
Toxoplasma Gondii ◽  
Free Ranging

Toxoplasma gondii Isolates From Free-Ranging Chickens From the United States

2003 ◽  
Vol 89 (5) ◽  
pp. 1060-1062 ◽  
Author(s):  
J. P. Dubey ◽  
D. H. Graham ◽  
E. Dahl ◽  
C. Sreekumar ◽  
T. Lehmann ◽  
...  
Keyword(s):  
United States ◽  
Toxoplasma Gondii ◽  
The United States ◽  
Free Ranging ◽  
Toxoplasma Gondii Isolates

scholarly journals Seroprevalence of Toxoplasma gondii in free-ranging and feral cats on Amami Oshima Island, Japan

2017 ◽  
Vol 79 (11) ◽  
pp. 1853-1856 ◽  
Author(s):  
Aya MATSUU ◽  
Shin-ichi YOKOTA ◽  
Keiko ITO ◽  
Tatsunori MASATANI
Keyword(s):  
Toxoplasma Gondii ◽  
Feral Cats ◽  
Free Ranging
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