Pseudo-Infected Red Blood Cell Beads as Positive Control for Cell Microarray Chip–Based Detection of Plasmodium-Infected RBCs

2018 ◽  
Vol 104 (3) ◽  
pp. 283-288
Author(s):  
Muneaki Hashimoto ◽  
Masahiko Numata ◽  
Shouki Yatsushiro ◽  
Yusuke Ido ◽  
Masato Tanaka ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Positive Control ◽  
Microarray Chip ◽  
Infected Rbcs ◽  
Cell Microarray

Uji Aktivitas Anti Inflamasi Ekstrak Bawang Dayak (Eleutherine Americana L. Merr) terhadap Stabilisasi Membran Sel Darah Merah

2019 ◽  
Vol 2 (1) ◽  
pp. 30
Author(s):  
Roji Septian Hardi ◽  
Slamet Slamet ◽  
Laila Kamilla
Keyword(s):  
Diabetes Mellitus ◽  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Diclofenac Sodium ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Purposive Sampling ◽  
Anti Inflammatory ◽  
Eleutherine Americana

Abstract:  Dayak onion plants (Eleutherine americana L. Merr) is one of the most common herbaceous plants used by the community as atraditional medicinal plants. Benefts of dayak onion as a medicine for various diseases such as breast cancer, diabetes mellitus, lowering hypertension, anti-inflammatory and lowering cholesterol.. Based on the research, Dayak bulb extract contains compounds such as Flavonoid, Phenol and Tanin are known to have anti-inflammatory activity. This study aims to determine the difference of anti-inflammatory activity of Dayak extract (Eleutherine americana L. Merr) compared with diclofenac sodium to stabilization of red blood cell membrane. This research is quasi experimental using stabilization method of red blood cell membrane. This study used purposive sampling technique with Dayak extract on concentration samples 0.005, 0.01, 0.02, 0.04 and 0.08% with the repetition of 5 times. Red blood cell lysis Inhibition induced by hipotonis solutionis used as an anti-inflammatory activity measurements. Anti-inflammatory activity of the extract is then compared to positive control (diclofenac sodium). The result of anti-inflammatory activity test showed that the dayak extract which has the highest anti-inflammatory activity was on the extract of 0.08% concentration which was 72.74%, while the most effective concentration of Dayak extract was at a Concentration of 0,02% that is equal to 59,58% of Tukey’s statistical results showed that 0.02% concentration did not differ signifcantly or identical with positive control (diclofenac sodium) at 0.01% concentration of 60.39% with a sample signifcance value of 0.757 ≥ α 0.05 which means that the onion dayak has potential as an antiinflammatoryAbstrak: Tanaman bawang dayak (Eleutherine americana L. Merr) merupakan salah satu jenis tanaman herbal semusim yang lazim digunakan oleh masyarakat sebagai tanaman obat tradisional. Manfaat tanaman bawang dayak sebagai obat berbagai jenis penyakit seperti kanker payudara, diabetes mellitus, menurunkan hipertensi, antiinflamasi dan menurunkan kadar kolesterol. Berdasarkan penelitian, ekstrak umbi bawang dayak mengandung senyawa berupa Flavonoid, Fenol dan Tanin yang diketahui memiliki aktivitas antiinflamasi. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas antiinflamasi ekstrak bawang dayak (Eleutherine americana L. Merr) yang dibandingkan dengan natrium diklofenak terhadap stabilisasi membran sel darah merah. Penelitian ini merupakan penelitian eksperimental semu dengan menggunakan metode stabilisasi membran sel darah merah. Penelitian ini menggunakan teknik purposive sampling dengan sampel ekstrak bawang dayak konsentrasi 0,005, 0,01, 0,02, 0,04 dan 0,08% dengan dilakukan pengulangan sebanyak 5. Penghambatan lisis sel darah merah akibat induksi larutan hipotonis digunakan sebagai ukuran aktivitas antiinflamasi. Aktivitas antiinflamasi dari ekstrak tersebut kemudian dibandingkan dengan kontrol positif (natriun diklofenak). Hasil uji aktivitas antiinflamasi menunjukkan bahwa ekstrak bawang dayak yang memiliki aktivitas antiinflamasi paling tinggi adalah pada ekstrak konsentrasi 0,08% yaitu sebesar 72,74%, sedangkan konsentrasi yang paling efektif dari ekstrak bawang dayak adalah pada konsentrasi 0,02% yaitu sebesar 59,58% dari hasil uji statistik Tukey menunjukkan pada konsentrasi 0,02% tidak berbeda secara bermakna atau identik dengan kontrol positif (natrium diklofenak) pada konsentrasi 0,01% yaitu sebesar 60,39% dengan nilai signifkansi sampel 0,757 ≥ 0,05 ini menunjukkan bahwa bawang dayak memiliki potensi sebagai antiinflamasi.

scholarly journals NEW MOLECULAR MECHANISM OF CEFTAZIDIME-INDUCED HUMAN RED BLOOD CELL HEMOLYSIS THROUGH THE PHOTOHEMOLYSIS REACTION

2018 ◽  
Vol 11 (15) ◽  
pp. 47
Author(s):  
Mashuri Mashuri ◽  
Achmad Zaini ◽  
Diana Rahmanisa ◽  
Muhammad Rifqi Alfiannoor ◽  
Muhammad Rosyhan Sanjaya ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Uv Light ◽  
Light Exposure ◽  
Positive Control ◽  
Negative Control ◽  
Group 3 ◽  
Group 2 ◽  
Experimental Group ◽  
Group 1

Objective: The present study was undertaken to investigate the photohemolysis reaction through photosensitization reaction by ceftazidime as a photosensitizer in human red blood cell (RBC).Methods: In this present study, human erythrocytes have used a sample. The sample then divided into six groups consisting of Group 1 (T1) served a negative control which consists of erythrocytes and buffers phosphate with pH 6.8; Group 2 (T2) served as a positive control which consists erythrocytes and buffers phosphate with pH 6.8 and exposed to UV-light; and Group 3, 4, 5, and 6 (T3, T4, T5, and T6) served as an experimental group which consists of erythrocytes, buffer phosphate with pH 6.8, ceftazidime with concentration 10%, 20%, 30%, and 40%, respectively, and also exposed to UV-light. UV-light exposure was done in 2 h. After the treatment period, the level of hydrogen peroxide (H2O2), conjugated diene (CD), advanced oxidation protein products (AOPPs), and percentage of RBC hemolysis (RBCH) were measured.Results: The results of this present studies showed that ceftazidime significantly increases the levels of H2O2, CD, AOPPs, and percentage of RBCH during the UV radiation.Conclusion: The present study demonstrated that ceftazidime acts as a photosensitizer and induced the photohemolysis reaction in human RBC. Furthermore, the hemolysis of RBC seems through the protein damage than lipid damage.

Continuous force-displacement relationships for the human red blood cell at different erythrocytic developmental stages ofPlasmodium falciparummalaria parasite

2004 ◽  
Vol 844 ◽  
Author(s):  
John P. Mills ◽  
Lan Qie ◽  
Ming Dao ◽  
Kevin S. W. Tan ◽  
Chwee Teck Lim ◽  
...  
Keyword(s):  
Blood Cell ◽  
Optical Tweezers ◽  
Red Blood Cell ◽  
Mechanical Response ◽  
Developmental Stages ◽  
Three Dimensional ◽  
Parasite Development ◽  
Human Red Blood Cells ◽  
Infected Rbcs ◽  
Order Of Magnitude

ABSTRACTPrior work involving either aspiration of infected cells into micropipette under suction pressure or deformation in laminar shear flow revealed that the malaria parasitePlasmodium (P.) falciparumcould result in significant stiffening of infected human red blood cells (RBCs). In this paper, we present optical tweezers studies of progressive changes to nonlinear mechanical response of infected RBCs at different developmental stages ofP. falciparum.From early ring stage to late trophozoite and schizont stages, up to an order of magnitude increase in shear modulus was found under controlled mechanical loading by combining experiments with three-dimensional computational simulations. These results provide novel approaches to study changes in mechanical deformability in the advanced stages of parasite development in the erythrocyte, and suggest a significantly greater stiffening of the red blood cell due toP. falciparuminvasion than that considered from previous studies.

scholarly journals Effect of Squid (Sepiotheutis lessoniana) Ink on Hematological Profile of Rats Rattus norvegicus

2020 ◽  
pp. 39-49
Author(s):  
Delianis Pringgenies ◽  
Meida M. M. Pertiwi ◽  
Ali Ridlo
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Iron Content ◽  
Rattus Norvegicus ◽  
Iron Deficiency Anaemia ◽  
Positive Control ◽  
Negative Control ◽  
Deficiency Anaemia ◽  
Low Dosage

Iron deficiency anaemia includes the highest incidence of nutritional deficiency diseases in the world. Red cell indices on full blood counts is regularly used to diagnose early anaemia. Currently strategies medicines of iron deficiency anaemia are belonged to preventif medicine as well as fortification of food and iron supplementation directly to the body. Recent studies show the effectiveness of treatment of iron deficiency anaemia in rats with squid ink. This study aim is to analyse the iron content of squid ink (Sepioteuthis lessoniana) and squid ink effects on haematological profile of male Wistar rat Rattus norvegicus. The measured iron content of squid ink (Sepioteuthis lessoniana) is 2,14 ± 0,46 mg/g. Rats were used in this study, are induced NaNO2 (50 mg/kgbw (1 ml dosing volume)) at first, then divided into 4 groups experiment for 14 days experimental. The 1st group is negative control (without any treatment), 2nd group is positive control (with Sangobion®), 3rd group is given a low dosage of squid ink (10 mg/kgbw/day) and the last 4thgroup is given a high dosage of squid ink (100 mg/kgbw/day). Based on the result, there was an effect of given squid ink to the haemoglobin, hematocrit, mean corpuscular volume, mean corpuscular haemoglobin concentration, mean haemoglobin content, red blood cell distribution width and red blood cell count of rats that showed by increasing numbers to the end of the experiment compared to the negative control (1st group). The most significant effect is in haemoglobin, especially Hb of rats which appeared on the low dosage of squid ink group and positive control group to the negatif group (p<0,05).

scholarly journals Anti-Inflammatory of Papaya Leaf Extract (Carica Papaya L) Towards Membrane Stabilization of Red Blood Cells

2021 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Laila Kamilla ◽  
Sri Tumpuk ◽  
Maulidiyah Salim
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Methanol Extract ◽  
Diclofenac Sodium ◽  
Positive Control ◽  
Modern Medicine ◽  
Inflammatory Activity ◽  
Quasi Experimental ◽  
Anti Inflammatory ◽  
Red Blood Cell Membranes

Traditional medical plants are known to society long ago. Apart from easily obtained and inexpensive, it can cure diseases with few side effects than modern medicine. Papaya leaves were used not only because of contained various chemical compounds with pharmacological effects but also alkaloids, flavonoids, saponins, and tannins compounds indicated as anti-inflammatory. This study applied a quasi-experimental design to test the papaya leaves anti-inflammatory activity. The red blood cell stabilization method was employed because analogous to the lysosomal membrane affected the inflammatory process. Purposive sampling was used, creating papaya leaves extract of 50 ppm, 100 ppm, 200 ppm, 400 ppm, 600 ppm, and 800 ppm concentration, made 24 total samples by four times replication. Based on the red blood cell lysis inhibition, the anti-inflammatory activity was measured and was compared with the positive control (diclofenac sodium). The papaya methanol extract result showed the highest anti-inflammatory activity at 800 ppm of 74.29%. The most effective concentration was at 200 ppm of 62.19%. Tukey's test showed p1.000 ≥ 0.05, suggesting H0 was accepted. There was no difference between the anti-inflammatory activity of papaya leaf methanol extract and diclofenac sodium showing stabilization of red blood cell membranes, indicating papaya potentially as an anti-inflammatory.

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