COMPARISON OF SUBCARAPACIAL SINUS AND BRACHIAL VEIN PHLEBOTOMY SITES FOR BLOOD COLLECTION IN FREE-RANGING GOPHER TORTOISES (GOPHERUS POLYPHEMUS)

2021 ◽  
Vol 52 (3) ◽  
Author(s):  
Donald L. Neiffer ◽  
Lee-Ann C. Hayek ◽  
Dianna Conyers ◽  
Andre Daneault ◽  
Jamie Sincage ◽  
...  
Keyword(s):  
Blood Collection ◽  
Gopherus Polyphemus ◽  
Brachial Vein ◽  
Free Ranging

Paecilomyces lilacinus Pneumonia in a Free-Ranging Gopher Tortoise, Gopherus polyphemus, in Louisiana

2008 ◽  
Vol 18 (2) ◽  
pp. 52-60 ◽  
Author(s):  
Orlando Diaz-Figueroa ◽  
Mark A. Mitchell ◽  
Samuel Ramirez ◽  
Wael Hananeh ◽  
Dae-Young Kim ◽  
...  
Keyword(s):  
Paecilomyces Lilacinus ◽  
Gopher Tortoise ◽  
Gopherus Polyphemus ◽  
Free Ranging

Daily Energy Expenditure in Free-ranging Gopher Tortoises (Gopherus Polyphemus)

Copeia ◽  
2006 ◽  
Vol 2006 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Patrick G. R. Jodice ◽  
Deborah M. Epperson ◽  
G. HenkVisser
Keyword(s):  
Energy Expenditure ◽  
Daily Energy Expenditure ◽  
Gopherus Polyphemus ◽  
Daily Energy ◽  
Free Ranging

scholarly journals Health and Reproductive Assessment of a Free-Ranging Gopher Tortoise (Gopherus polyphemus) Population Following Translocation

2010 ◽  
Vol 20 (2) ◽  
pp. 84 ◽  
Author(s):  
Sonia M. Hernandez ◽  
Tracey D. Tuberville ◽  
Paul Frank ◽  
Scott J. Stahl ◽  
Michael M. McBride ◽  
...  
Keyword(s):  
Gopher Tortoise ◽  
Gopherus Polyphemus ◽  
Free Ranging

scholarly journals Comparison of African savanna elephant (Loxodonta africana) fatty acid profiles in whole blood, whole blood dried on blood spot cards, serum, and plasma

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12650
Author(s):  
Jordan Wood ◽  
Larry J. Minter ◽  
Doug Bibus ◽  
Michael K. Stoskopf ◽  
Vivek Fellner ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Whole Blood ◽  
Health Assessment ◽  
Field Research ◽  
Blood Collection ◽  
Fatty Acid Profiles ◽  
Omega 3 ◽  
African Elephants ◽  
Free Ranging

Background African elephants in managed care have presented differences in the balance between omega-3 and omega-6 fatty acids, a situation primarily thought to be due to dietary differences between the managed animals and their free-ranging counterparts. Because of this, circulating fatty acid status is included in routine monitoring of elephant health. A method of blood collection that requires only a few drops of whole blood, dried on filter paper (DBS) and can be used for analyzing full fatty acid profiles offers advantages in clinical application. Methods This study compared the use of whole blood, and whole blood DBS, serum or plasma for use in evaluating circulating fatty acid composition in African savannah elephants. Samples from six African elephants (two males and four females) were collected during the same week at the NC Zoo, Asheboro, NC. Results Results found only 2 of 36 individual fatty acids and none of the 10 fatty acid groupings were different when comparing the four blood fraction sample types to each other with Mann-Whitney U-Test pairwise comparisons. Myristic acid (14:0) was lower in the DBS samples than in whole blood, serum, and plasma and pentadecaenoic acid (15:1) was slightly more concentrated in DBS and whole blood. Discussion Results indicate that fatty acid profile of serum, plasma, whole blood, and DBS are comparable in African elephants. The DBS method offers advantages in acquisition and handling and may be preferable to other methods in both routine health assessment of captive animals and field research on free ranging animals.

Stability of antioxidant vitamins in whole human blood during overnight storage at 4°C and frozen storage up to 6 months

2018 ◽  
Vol 88 (3-4) ◽  
pp. 151-157 ◽  
Author(s):  
Scott W. Leonard ◽  
Gerd Bobe ◽  
Maret G. Traber
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Healthy Subjects ◽  
Frozen Storage ◽  
Blood Collection ◽  
Plasma Samples ◽  
Optimal Conditions ◽  
Plasma Ascorbic Acid ◽  
Blood Samples ◽  
Sample Handling

Abstract. To determine optimal conditions for blood collection during clinical trials, where sample handling logistics might preclude prompt separation of erythrocytes from plasma, healthy subjects (n=8, 6 M/2F) were recruited and non-fasting blood samples were collected into tubes containing different anticoagulants (ethylenediaminetetra-acetic acid (EDTA), Li-heparin or Na-heparin). We hypothesized that heparin, but not EDTA, would effectively protect plasma tocopherols, ascorbic acid, and vitamin E catabolites (α- and γ-CEHC) from oxidative damage. To test this hypothesis, one set of tubes was processed immediately and plasma samples were stored at −80°C, while the other set was stored at 4°C and processed the following morning (~30 hours) and analyzed, or the samples were analyzed after 6 months of storage. Plasma ascorbic acid, as measured using HPLC with electrochemical detection (LC-ECD) decreased by 75% with overnight storage using EDTA as an anticoagulant, but was unchanged when heparin was used. Neither time prior to processing, nor anticoagulant, had any significant effects upon plasma α- or γ-tocopherols or α- or γ-CEHC concentrations. α- and γ-tocopherol concentrations remained unchanged after 6 months of storage at −80°C, when measured using either LC-ECD or LC/mass spectrometry. Thus, refrigeration of whole blood at 4°C overnight does not change plasma α- or γ-tocopherol concentrations or their catabolites. Ascorbic acid is unstable in whole blood when EDTA is used as an anticoagulant, but when whole blood is collected with heparin, it can be stored overnight and subsequently processed.

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