Real-Time Polymerase Chain Reaction Testing for the Detection of Mycobacterium genavense and Mycobacterium avium Complex Species in Avian Samples

10.1637/7063 ◽  
2003 ◽  
Vol 47 (4) ◽  
pp. 1406-1415 ◽  
Author(s):  
Lisa A. Tell ◽  
Christian M. Leutenegger ◽  
R. Scott Larsen ◽  
Dalen W. Agnew ◽  
Laura Keener ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Complex ◽  
Chain Reaction ◽  
Complex Species ◽  
Polymerase Chain Reaction Testing ◽  
Mycobacterium Genavense ◽  
Polymerase Chain

scholarly journals CT Manifestations and Clinical Features of the 2019 Novel Coronavirus Pneumonia Infected by Cluster Transmission Within a Family: Case Report

2020 ◽  
Author(s):  
Jin-ming Cao ◽  
Yu-ping Wu ◽  
Tian-wu Chen ◽  
Li-qin Yang ◽  
Xiao-ming Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Close Contact ◽  
Clinical Signs ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Epidemic Area ◽  
History Of ◽  
Polymerase Chain ◽  
Novel Coronavirus

Abstract Background: In December 2019, a cluster of patients associated with a seafood wholesale market was confirmed having infected the 2019 novel coronavirus (2019-nCoV) in Wuhan, China. As of Feb 11, 2020, 43144 cases of the 2019-nCoV infection have been confirmed in the world, and person-to-person transmission has been recognized. To our knowledge, there are no reports regarding the 2019-nCoV pneumonia infected by cluster transmission within a family. The amount of close contact suspect was increasing. We reported cases of family cluster transmission of the 2019-nCoV infection, showing the differences in computed tomography (CT) manifestations and symptoms between patients with and without history of exposure to the epidemic area (Wuhan).Case Presentation: A 48-year-old man was presented to the hospital in Jan 30, 2020 with a 2-day history of low fever and chill. He had traveled to Wuhan City of Hubei Province of China 12 days before, and was confirmed having the 2019-nCoV infection based on his positive CT manifestations, clinical signs, and real-time fluorescence polymerase chain reaction results. The other three members of his family without history of exposure to the epidemic area (Wuhan) were subsequently identified having the 2019-nCoV transmissive infection based on the positive findings of real-time fluorescence polymerase chain reaction, but they did not have abnormal CT manifestations and clinical signs.Conclusion: For patients who have history of exposure to the epidemic area (Wuhan), the 2019-nCoV infected pneumonia can be identified by real-time fluorescence polymerase chain reaction testing and chest CT together with the symptoms. But for patients without exposure to the epidemic area, the 2019-nCoV infection can be confirmed by real-time fluorescence polymerase chain reaction testing and history of close contact with confirmed patients who have history of exposure to the epidemic area.

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