Incidence and Tracking of Clostridium perfringens Through an Integrated Broiler Chicken Operation

10.1637/6010 ◽  
2003 ◽  
Vol 47 (3) ◽  
pp. 707-711 ◽  
Author(s):  
S. E. Craven ◽  
N. A. Cox ◽  
J. S. Bailey ◽  
D. E. Cosby
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chicken

scholarly journals Structural analysis of broiler chicken small intestinal mucin O-glycan modification by Clostridium perfringens

2019 ◽  
Vol 98 (10) ◽  
pp. 5074-5088 ◽  
Author(s):  
Jaclyn L MacMillan ◽  
Sara D Vicaretti ◽  
Benjamin Noyovitz ◽  
Xiaohui Xing ◽  
Kristin E Low ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Clostridium Perfringens ◽  
Broiler Chicken ◽  
Intestinal Mucin ◽  
Small Intestinal ◽  
Glycan Modification

scholarly journals Multilocus Sequence Typing Analysis of Clostridium perfringens Isolates from Necrotic Enteritis Outbreaks in Broiler Chicken Populations

2008 ◽  
Vol 46 (12) ◽  
pp. 3957-3964 ◽  
Author(s):  
G. Chalmers ◽  
H. L. Bruce ◽  
D. B. Hunter ◽  
V. R. Parreira ◽  
R. R. Kulkarni ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Multilocus Sequence Typing ◽  
Broiler Chicken ◽  
Necrotic Enteritis

scholarly journals Isolation, identification and toxinotyping of Clostridium perfringens isolated from broilers in Pakistan

2021 ◽  
Vol 58 (04) ◽  
pp. 1367-1372
Author(s):  
Zain Ul Abadeen
Keyword(s):  
Clostridium Perfringens ◽  
Causative Agent ◽  
Broiler Chicken ◽  
Clinical Signs ◽  
Type A ◽  
Toxin Gene ◽  
Necrotic Enteritis ◽  
Alpha Toxin ◽  
Enteric Disease ◽  
Isolation Rate

Necrotic enteritis (NE) is one of the important enteric disease in the poultry industry worldwide, caused by C. perfringens type A. This study describes the isolation, identification, and toxinotyping of C. perfringens in necrotic enteritis affected broiler chicken in Pakistan. A total of 430 intestinal samples from dead carcasses and birds suspected of NE outbreak, in and around Faisalabad, Pakistan were collected from 36 broiler farms which yielded 87 alpha toxin gene (cpa) positive C. perfringens type A isolates. The birds having 4-5 weeks of age, clinical signs, and reared in open (conventional) sheds showed higher C. perfringens isolation rate. The study concluded netB negative C. perfringens type A as a causative agent for NE outbreaks in broiler birds in Faisalabad, Pakistan.

Clostridium perfringens Type A from Broiler Chicken with Necrotic Enteritis

2008 ◽  
Vol 7 (6) ◽  
pp. 601-609 ◽  
Author(s):  
Arunava Das ◽  
Yahya Mazumder ◽  
Biman Kumar Dutta ◽  
Bibek Ranjan Shome ◽  
Komal Molla Bujarbarua ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chicken ◽  
Type A ◽  
Necrotic Enteritis

scholarly journals Detection of a Group II Intron without an Open Reading Frame in the Alpha-Toxin Gene of Clostridium perfringens Isolated from a Broiler Chicken

2006 ◽  
Vol 189 (5) ◽  
pp. 1633-1640 ◽  
Author(s):  
Menglin Ma ◽  
Kaori Ohtani ◽  
Tohru Shimizu ◽  
Naoaki Misawa
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chicken ◽  
Group Ii Intron ◽  
Open Reading Frame ◽  
Start Codon ◽  
Northern Hybridization ◽  
Toxin Gene ◽  
Alpha Toxin ◽  
Reading Frame ◽  
Group Ii

ABSTRACTA DNA insertion of 834 bp, designated CPF-G2Im, was identified within the alpha toxin gene (cpa) ofClostridium perfringensstrain CPBC16ML, isolated from a broiler chicken. Sequence analysis of CPF-G2Im indicated that it was integrated 340 nucleotides downstream of the start codon ofcpa. However, the insertion did not abolish the phospholipase C and hemolytic activities of CPBC16ML. To investigate the expression of its alpha toxin, the intact copy ofcpawas cloned into an expression vector and transformed intoEscherichia coliM15 cells. Immunoblotting analysis showed that the protein expressed from the transformant as well as in the culture supernatant ofC. perfringensstrain CPBC16ML had the expected molecular weight detected in reference strains ofC. perfringens. Northern hybridization and reverse transcriptase PCR (RT-PCR) analysis revealed that the entire CPF-G2Im insertion was completely spliced from thecpaprecursor mRNA transcripts. The sequence of the insertion fragment has 95% and 97% identity to two noncoding regions corresponding to sequences that flank a predicted group II RT gene present in the pCPF4969 plasmid ofC. perfringens. However, an RT was not encoded by the CPF-G2Im fragment. Based on the secondary structure prediction analysis, CPF-G2Im revealed typical features of group II introns. The present study shows that CPF-G2Im is capable of splicing in bothC. perfringensandE. coli. To our knowledge, this is the first report that a group II intron without an open reading frame (ORF) is located in thecpaORF ofC. perfringens.

Sign in / Sign up

Export Citation Format

Share Document