The Role of Litter Beetles as Potential Reservoir for Salmonella enterica and Thermophilic Campylobacter spp. Between Broiler Flocks

M. N. Skov; A. G. Spencer; B. Hald; L. Petersen; B. Nauerby; B. Carstensen; M. Madsen

doi:10.1637/5698

The prevalence of virulence genes and multidrug resistance in thermophilic Campylobacter spp. isolated from dogs

Open Life Sciences ◽

10.1515/biol-2019-0077 ◽

2019 ◽

Vol 14 (1) ◽

pp. 681-687

Author(s):

Marek Selwet

Keyword(s):

Multidrug Resistance ◽

Virulence Genes ◽

Age Groups ◽

Cytolethal Distending Toxin ◽

Frequency Of Occurrence ◽

Low Resistance ◽

Thermophilic Campylobacter ◽

Potential Reservoir ◽

Campylobacter Spp

AbstractThe aim of the study was to determine the role of dogs as a potential reservoir of Campylobacter spp. At the next stage of the research the frequency of occurrence of selected virulence genes, i.e. cadF, flaA and iam as well as genes responsible for the formation the cytolethal distending toxin (CDT), i.e. cdtA, cdtB and cdtC was determined. The isolates obtained in the research were tested for their resistance to selected antibiotics: ciprofloxacin (CIP), enrofloxacin (EF), erythromycin (E) and tetracycline (TE). Campylobacter spp. was found in 63 (12.6%) out of a total number of 500 isolates. 61 (12.2%) isolates were identified as C. jejuni. The number of C. jejuni isolates found in the younger animals was smaller (p <0.05) than in the older ones. The frequency of occurrence of virulence genes and the genes responsible for the formation of CDT was significantly (p <0.05) higher in the older dogs. A comparison of the effect of antibiotics showed that the isolates obtained from both age groups exhibited low resistance to erythrosine (13.5% in the group aged under 1 year and 8.6% in the group aged over 1 year). Both groups exhibited the highest resistance to ciprofloxacin and enrofloxacin.

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Prevalence of Campylobacter jejuni and Campylobacter coli species in cats and dogs from Bydgoszcz (Poland) region

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0016 ◽

2013 ◽

Vol 16 (1) ◽

pp. 115-120 ◽

Cited By ~ 14

Author(s):

M. Andrzejewska ◽

B. Szczepańska ◽

J.J. Klawe ◽

D. Śpica ◽

M. Chudzińska

Keyword(s):

Virulence Genes ◽

Random Amplified Polymorphic Dna ◽

Campylobacter Coli ◽

Small Water ◽

Private Households ◽

High Prevalence ◽

Potential Reservoir ◽

Autumn And Winter ◽

Campylobacter Spp

AbstractThe aim of this study was to investigate the role of cats and dogs as a potential reservoir of Campylobacter spp. Rectal swabs from 83 dogs and 71 cats were examined. Samples were obtained from the animals aged between 2 weeks and 24 months living in shelters, private households, farms and from veterinary clinics located in Bydgoszcz region during routine check-up.Campylobacter spp. were isolated from 4.81% dogs and 9.86% cats, respectively. C. jejuni was predominant in this study. All strains were isolated in autumn and winter from the animals living in farms and private houses. All the animals positive for Campylobacter prevalence had access to small water basins, accidental source of food and had contact with wild birds, poultry or their feaces.Isolates characterization revealed high prevalence of Campylobacter virulence genes-flaA, cadF and cdtB. 91% of isolated strains were susceptible to erythromycin. 81% among isolated strains were susceptible to azithromycin, 64% to tetracycline and 36% to ciprofloxacin. For 2 C. jejuni strains isolated from cats Random Amplified Polymorphic DNA (RAPD) profiling indicated 80% homology between them.

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Prevalence and Antibiotic Resistance of Campylobacter spp. in Urban and Rural Black-Headed Gulls Chroicocephalus ridibundus

EcoHealth ◽

10.1007/s10393-021-01540-0 ◽

2021 ◽

Author(s):

Piotr Indykiewicz ◽

Małgorzata Andrzejewska ◽

Piotr Minias ◽

Dorota Śpica ◽

Jarosław Kowalski

Keyword(s):

Animal Health ◽

Campylobacter Coli ◽

Anthropogenic Pressures ◽

Rural And Urban ◽

Potential Reservoir ◽

Campylobacter Lari ◽

Almost All ◽

Breeding Seasons ◽

Campylobacter Spp

AbstractWe investigate the role of black-headed gulls (Chroicocephalus ridibundus), an omnivorous species that is among the most likely wild bird candidates for transmission of zoonotic agents, as a potential reservoir of Campylobacter spp. Colonies with different anthropogenic pressures were studied to examine differences in exposure to sources of Campylobacter between rural and urban birds. We recorded Campylobacter spp. in 4.87% of adult black-headed gulls and 2.22% of their chicks after analysing 1036 cloacal swabs collected over two breeding seasons in three colonies in northern Poland. Campylobacter jejuni was found most frequently (85.72%), and Campylobacter lari and Campylobacter coli were much scarcer. Prevalence of Campylobacter did not differ significantly between black-headed gulls breeding in urban (4.27%) and rural (3.80%) habitats. Almost all isolates from chicks and adults were susceptible to azithromycin (97.62%) and erythromycin (95.24%), but fewer to tetracycline (50.00%) and ciprofloxacin (47.62%). Campylobacter prevalence was unrelated to the date of sampling. Our study indicates that black-headed gulls are carriers of resistant to antibiotics Campylobacter and they can contaminate natural waterbodies with their faeces, which poses a threat to human and farm animal health.

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Dogs and Cats: Reservoirs for Highly Diverse Campylobacter jejuni and a Potential Source of Human Exposure

Animals ◽

10.3390/ani10050838 ◽

2020 ◽

Vol 10 (5) ◽

pp. 838

Author(s):

Amandine Thépault ◽

Valérie Rose ◽

Marilyne Queguiner ◽

Marianne Chemaly ◽

Katell Rivoal

Keyword(s):

Genetic Diversity ◽

Campylobacter Jejuni ◽

Human Exposure ◽

High Genetic Diversity ◽

Thermophilic Campylobacter ◽

Potential Link ◽

Genomic Characterization ◽

Partial Overlap ◽

Campylobacter Spp

Assessing the carriage of Campylobacter in animal reservoirs is essential to better understand Campylobacter epidemiology. Here, we evaluated the prevalence of thermophilic Campylobacter spp. in dogs and cats, hereafter defined as pets, and characterized Campylobacter jejuni (C. jejuni) isolates to assess their genetic diversity and their potential link with isolates from other animals or human cases. During a 6-month period, 304 feces samples were collected from pets. A significantly higher prevalence of thermophilic Campylobacter spp. was found in dogs compared with cats, as well as in dogs ≤ 1-year-old compared with older dogs. C. jejuni was the predominant species found in pets, and its genomic characterization revealed a high genetic diversity. Genotypes comparison with previously characterized isolates revealed a partial overlap between C. jejuni isolates from pets, chicken, cattle, and clinical cases. This overlap suggests the potential role of livestock and humans in pets’ exposure to Campylobacter, or vice versa. The isolation of pets’ specific profiles may suggest the existence of other sources of pet contamination or imply that pets may constitute a reservoir for Campylobacter. Because of the proximity between humans and pets, along with their frequent carriage of C. jejuni, human exposure to Campylobacter from pets can be more important than previously thought.

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Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

Acta veterinaria ◽

10.1515/acve-2016-0017 ◽

2016 ◽

Vol 66 (2) ◽

pp. 203-213 ◽

Cited By ~ 1

Author(s):

Dragana Jošić ◽

Jelena Petković ◽

Olivera Bunčić ◽

Zorica Lepšanović ◽

Radmila Pivić ◽

...

Keyword(s):

Random Amplified Polymorphic Dna ◽

Strain Identification ◽

Broiler Meat ◽

Thermophilic Campylobacter ◽

Campylobacter Species ◽

The Republic ◽

Human Infections ◽

Different Levels ◽

Campylobacter Spp

Abstract Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.

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