Skeletal Muscle Fiber-Specific Green Autofluorescence: Potential for Stem Cell Engraftment Artifacts

Stem Cells ◽  
2004 ◽  
Vol 22 (2) ◽  
pp. 180-187 ◽  
Author(s):  
Kathyjo A. Jackson ◽  
D. Scott Snyder ◽  
Margaret A. Goodell
Keyword(s):  
Skeletal Muscle ◽  
Stem Cell ◽  
Muscle Fiber ◽  
Skeletal Muscle Fiber ◽  
Cell Engraftment

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

Confocal microscopy study of membrane organelles of the skeletal muscle fiber in the process of Zenker’s (spreading) necrosis

2007 ◽  
Vol 1 (2) ◽  
pp. 183-190 ◽  
Author(s):  
S. A. Krolenko ◽  
S. Ya. Adamyan ◽  
T. N. Belyaeva ◽  
T. P. Mozhenok ◽  
A. V. Salova
Keyword(s):  
Skeletal Muscle ◽  
Confocal Microscopy ◽  
Muscle Fiber ◽  
Microscopy Study ◽  
Skeletal Muscle Fiber

scholarly journals Skeletal muscle fiber size and fiber type distribution in human cancer: Effects of weight loss and relationship to physical function

2016 ◽  
Vol 35 (6) ◽  
pp. 1359-1365 ◽  
Author(s):  
Michael J. Toth ◽  
Damien M. Callahan ◽  
Mark S. Miller ◽  
Timothy W. Tourville ◽  
Sarah B. Hackett ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Weight Loss ◽  
Physical Function ◽  
Muscle Fiber ◽  
Human Cancer ◽  
Fiber Type ◽  
Skeletal Muscle Fiber ◽  
Type Distribution ◽  
Fiber Size ◽  
Fiber Type Distribution
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