scholarly journals Celebrating 10 Years of hESC Lines: An Interview with James Thomson

Stem Cells ◽  
2008 ◽  
Vol 26 (11) ◽  
pp. 2747-2748
Author(s):  
Miodrag Stojković ◽  
Susan Rainey Daher
Keyword(s):  
James Thomson ◽  
Hesc Lines

James Thomson, L.D.S

BDJ ◽  
1976 ◽  
Vol 141 (1) ◽  
pp. 29-29
Keyword(s):  
James Thomson

Who Was “Cenus” in the Poem To the Memory of Mr. Congreve?

PMLA ◽  
1929 ◽  
Vol 44 (2) ◽  
pp. 495-500
Author(s):  
George G. Williams
Keyword(s):  
James Thomson ◽  
Important Evidence

The poem To the Memory of Mr. Congreve, first printed in 1729, is usually attributed to James Thomson, although his authorship of it has been questioned. This poem was not reprinted until 1843, since which date some editors have included it in their collections of Thomson's works, and some have excluded it. I hope soon to present some hitherto unrecorded evidence that this poem was written by Thomson, and accordingly in the present paper I proceed on the assumption that he was its author. At the same time, as I shall try to show, the conclusion which this paper seeks to establish will itself supply important evidence in support of Thomson's authorship.

scholarly journals MFN2 Deficiency Impairs Mitochondrial Transport and Downregulates Motor Protein Expression in Human Spinal Motor Neurons

2021 ◽  
Vol 14 ◽  
Author(s):  
Yongchao Mou ◽  
Joshua Dein ◽  
Zhenyu Chen ◽  
Mrunali Jagdale ◽  
Xue-Jun Li
Keyword(s):  
Protein Expression ◽  
Motor Neurons ◽  
Axonal Degeneration ◽  
Axonal Neuropathy ◽  
Motor Protein ◽  
Mitochondrial Morphology ◽  
Mitochondrial Transport ◽  
Spinal Motor Neurons ◽  
Spinal Motor ◽  
Hesc Lines

Charcot-Marie-Tooth (CMT) disease is one of the most common genetically inherited neurological disorders and CMT type 2A (CMT 2A) is caused by dominant mutations in the mitofusin-2 (MFN2) gene. MFN2 is located in the outer mitochondrial membrane and is a mediator of mitochondrial fusion, with an essential role in maintaining normal neuronal functions. Although loss of MFN2 induces axonal neuropathy, the detailed mechanism by which MFN2 deficiency results in axonal degeneration of human spinal motor neurons remains largely unknown. In this study, we generated MFN2-knockdown human embryonic stem cell (hESC) lines using lentivirus expressing MFN2 short hairpin RNA (shRNA). Using these hESC lines, we found that MFN2 loss did not affect spinal motor neuron differentiation from hESCs but resulted in mitochondrial fragmentation and dysfunction as determined by live-cell imaging. Notably, MFN2-knockodwn spinal motor neurons exhibited CMT2A disease-related phenotypes, including extensive perikaryal inclusions of phosphorylated neurofilament heavy chain (pNfH), frequent axonal swellings, and increased pNfH levels in long-term cultures. Importantly, MFN2 deficit impaired anterograde and retrograde mitochondrial transport within axons, and reduced the mRNA and protein levels of kinesin and dynein, indicating the interfered motor protein expression induced by MFN2 deficiency. Our results reveal that MFN2 knockdown induced axonal degeneration of spinal motor neurons and defects in mitochondrial morphology and function. The impaired mitochondrial transport in MFN2-knockdown spinal motor neurons is mediated, at least partially, by the altered motor proteins, providing potential therapeutic targets for rescuing axonal degeneration of spinal motor neurons in CMT2A disease.

The Pessimism of James Thomson (B.V.) in Relation to His Times

1968 ◽  
Vol 63 (2) ◽  
pp. 467
Author(s):  
Leonee Ormond ◽  
Kenneth Hugh Byron ◽  
William David Schaefer ◽  
Charles Vachot
Keyword(s):  
James Thomson
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