Ciliary Neurotrophic Factor-Mediated Signaling Regulates Neuronal Versus Glial Differentiation of Retinal Stem Cells/Progenitors by Concentration-Dependent Recruitment of Mitogen-Activated Protein Kinase and Janus Kinase-Signal Transducer and Activator of

Stem Cells ◽  
2008 ◽  
Vol 26 (10) ◽  
pp. 2611-2624 ◽  
Author(s):  
Sumitra Bhattacharya ◽  
Ani V. Das ◽  
Kavita B. Mallya ◽  
Iqbal Ahmad
Keyword(s):  
Stem Cells ◽  
Protein Kinase ◽  
Neurotrophic Factor ◽  
Ciliary Neurotrophic Factor ◽  
Janus Kinase ◽  
Mitogen Activated Protein Kinase ◽  
Signal Transducer ◽  
Glial Differentiation ◽  
Mitogen Activated Protein ◽  
Retinal Stem Cells

scholarly journals Borrelia burgdorferi-Induced Expression of Matrix Metalloproteinases from Human Chondrocytes Requires Mitogen-Activated Protein Kinase and Janus Kinase/Signal Transducer and Activator of Transcription Signaling Pathways

2004 ◽  
Vol 72 (5) ◽  
pp. 2864-2871 ◽  
Author(s):  
Aruna K. Behera ◽  
Cheleste M. Thorpe ◽  
J. Michael Kidder ◽  
Wendy Smith ◽  
Ethan Hildebrand ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Janus Kinase ◽  
Lyme Arthritis ◽  
Mitogen Activated Protein Kinase ◽  
Cartilage Explants ◽  
Human Chondrocytes ◽  
Signal Transducer ◽  
Tnf Α ◽  
Mitogen Activated Protein ◽  
Infected Cells

ABSTRACT Elevations in matrix metalloproteinase 1 (MMP-1) and MMP-3 have been found in patients with Lyme arthritis and in in vitro models of Lyme arthritis using cartilage explants and chondrocytes. The pathways by which B. burgdorferi, the causative agent of Lyme disease, induces the production of MMP-1 and MMP-3 have not been elucidated. We examined the role of the extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK) and the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathways in MMP induction by B. burgdorferi. Infection with B. burgdorferi results in rapid phosphorylation of p38 and JNK within 15 to 30 min. Inhibition of JNK and p38 MAPK significantly reduced B. burgdorferi-induced MMP-1 and MMP-3 expression. Inhibition of ERK1/2 completely inhibited the expression of MMP-3 in human chondrocytes following B. burgdorferi infection but had little effect on the expression of MMP-1. B. burgdorferi infection also induced phosphorylation and nuclear translocation of STAT-3 and STAT-6 in primary human chondrocytes. Expression of MMP-1 and MMP-3 was significantly inhibited by inhibition of JAK3 activity. Induction of MMP-1 and -3 following MAPK and JAK/STAT activation was cycloheximide sensitive, suggesting synthesis of intermediary proteins is required. Inhibition of tumor necrosis factor alpha (TNF-α) significantly reduced MMP-1 but not MMP-3 expression from B. burgdorferi-infected cells; inhibition of interleukin-1β (IL-1β) had no effect. Treatment of B. burgdorferi-infected cells with JAK and MAPK inhibitors significantly inhibited TNF-α induction, consistent with at least a partial role for TNF-α in B. burgdorferi-induced MMP-1 expression in chondrocytes.

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