Spermatogonial Stem Cell Self-Renewal Requires OCT4, a Factor Downregulated During Retinoic Acid-Induced Differentiation

Stem Cells ◽  
2008 ◽  
Vol 26 (11) ◽  
pp. 2928-2937 ◽  
Author(s):  
Christina Tenenhaus Dann ◽  
Alma L. Alvarado ◽  
Laura A. Molyneux ◽  
Bray S. Denard ◽  
David L. Garbers ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinoic Acid ◽  
Spermatogonial Stem Cell ◽  
Induced Differentiation ◽  
Self Renewal

scholarly journals Interaction of retinoic acid and scl controls primitive blood development

Blood ◽  
2010 ◽  
Vol 116 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Jill L. O. de Jong ◽  
Alan J. Davidson ◽  
Yuan Wang ◽  
James Palis ◽  
Praise Opara ◽  
...  
Keyword(s):  
Stem Cell ◽  
Retinoic Acid ◽  
Cell Fate ◽  
Embryonic Stem ◽  
Yolk Sac ◽  
Erythroid Progenitors ◽  
Self Renewal ◽  
Hematopoietic Development ◽  
Blood Island ◽  
Primitive Hematopoiesis

Abstract Hematopoietic development during embryogenesis involves the interaction of extrinsic signaling pathways coupled to an intrinsic cell fate that is regulated by cell-specific transcription factors. Retinoic acid (RA) has been linked to stem cell self-renewal in adults and also participates in yolk sac blood island formation. Here, we demonstrate that RA decreases gata1 expression and blocks primitive hematopoiesis in zebrafish (Danio rerio) embryos, while increasing expression of the vascular marker, fli1. Treatment with an inhibitor of RA biosynthesis or a retinoic acid receptor antagonist increases gata1+ erythroid progenitors in the posterior mesoderm of wild-type embryos and anemic cdx4−/− mutants, indicating a link between the cdx-hox signaling pathway and RA. Overexpression of scl, a DNA binding protein necessary for hematopoietic development, rescues the block of hematopoiesis induced by RA. We show that these effects of RA and RA pathway inhibitors are conserved during primitive hematopoiesis in murine yolk sac explant cultures and embryonic stem cell assays. Taken together, these data indicate that RA inhibits the commitment of mesodermal cells to hematopoietic fates, functioning downstream of cdx4 and upstream of scl. Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation.

scholarly journals Genetic Influences in Mouse Spermatogonial Stem Cell Self-Renewal

2010 ◽  
Vol 56 (1) ◽  
pp. 145-153 ◽  
Author(s):  
Mito KANATSU-SHINOHARA ◽  
Narumi OGONUKI ◽  
Hiromi MIKI ◽  
Kimiko INOUE ◽  
Hiroko MORIMOTO ◽  
...  
Keyword(s):  
Stem Cell ◽  
Spermatogonial Stem Cell ◽  
Genetic Influences ◽  
Self Renewal

Retinoic acid triggers c-kit gene expression in spermatogonial stem cells through an enhanceosome constituted between transcription factor binding sites for retinoic acid response element (RARE), spleen focus forming virus proviral integration oncogene (SPFI1) (PU.1) and E26 transformation-specific (ETS)

2017 ◽  
Vol 29 (3) ◽  
pp. 521 ◽  
Author(s):  
Swanand Koli ◽  
Ayan Mukherjee ◽  
Kudumula Venkata Rami Reddy
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Retinoic Acid ◽  
Binding Sites ◽  
Promoter Region ◽  
Spermatogonial Stem Cell ◽  
Proviral Integration ◽  
Response Element ◽  
Retinoic Acid Response Element ◽  
Kit Gene

Restricted availability of retinoic acid (RA) in the testicular milieu regulates transcriptional activity of c-kit (KIT, CD117), which aids in the determination of spermatogonial stem-cell differentiation. The effect of RA on c-kit has been reported previously, but its mode of genomic action remains unresolved. We studied the molecular machinery guiding RA responsiveness to the c-kit gene using spermatogonial stem-cell line C18–4 and primary spermatogonial cells. A novel retinoic acid response element (RARE) positioned at –989 nucleotides upstream of the transcription start site (TSS) was identified, providing a binding site for a dimeric RA receptor (i.e. retinoic acid receptor gamma (RARγ) and retinoic X receptor). RA treatment influenced c-kit promoter activity, along with endogenous c-kit expression in C18–4 cells. A comprehensive promoter deletion assay using the pGL3B reporter system characterised the region spanning –271 bp and –1011 bp upstream of the TSS, which function as minimal promoter and maximal promoter, respectively. In silico analysis predicted that the region –1011 to +58 bp comprised the distal enhancer RARE and activators such as spleen focus forming virus proviral integration oncogene (SPFI1) (PU.1), specificity protein 1 (SP1) and four E26 transformation-specific (ETS) tandem binding sites at the proximal region. Gel retardation and chromatin immunoprecipitation (ChIP) assays showed binding for RARγ, PU.1 and SP1 to the predicted consensus binding sequences, whereas GABPα occupied only two out of four ETS binding sites within the c-kit promoter region. We propose that for RA response, an enhanceosome is orchestrated through scaffolding of a CREB-binding protein (CBP)/p300 molecule between RARE and elements in the proximal promoter region, controlling germ-line expression of the c-kit gene. This study outlines the fundamental role played by RARγ, along with other non-RAR transcription factors (PU.1, SP1 and GABPα), in the regulation of c-kit expression in spermatogonial stem cells in response to RA.

scholarly journals Genetic Reconstruction of Mouse Spermatogonial Stem Cell Self-Renewal In Vitro by Ras-Cyclin D2 Activation

2009 ◽  
Vol 5 (1) ◽  
pp. 76-86 ◽  
Author(s):  
Jiyoung Lee ◽  
Mito Kanatsu-Shinohara ◽  
Hiroko Morimoto ◽  
Yasuhiro Kazuki ◽  
Seiji Takashima ◽  
...  
Keyword(s):  
Stem Cell ◽  
Spermatogonial Stem Cell ◽  
Cyclin D2 ◽  
Self Renewal

scholarly journals FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation

Development ◽  
2012 ◽  
Vol 139 (10) ◽  
pp. 1734-1743 ◽  
Author(s):  
K. Ishii ◽  
M. Kanatsu-Shinohara ◽  
S. Toyokuni ◽  
T. Shinohara
Keyword(s):  
Stem Cell ◽  
Spermatogonial Stem Cell ◽  
Self Renewal

scholarly journals Spermatogonial Stem Cell Self - renewal and Differentiation

2017 ◽  
Vol 1 (3) ◽  
pp. 171 ◽  
Author(s):  
Li-Huan Cao ◽  
Xin-Hua Lin ◽  
Qiao-Li Zhang
Keyword(s):  
Stem Cell ◽  
Spermatogonial Stem Cell ◽  
Self Renewal
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