scholarly journals Efficient In Vitro Labeling of Human Neural Precursor Cells with Superparamagnetic Iron Oxide Particles: Relevance for In Vivo Cell Tracking

Stem Cells ◽  
2008 ◽  
Vol 26 (2) ◽  
pp. 505-516 ◽  
Author(s):  
Margherita Neri ◽  
Claudio Maderna ◽  
Chiara Cavazzin ◽  
Vivien Deidda-Vigoriti ◽  
Letterio S. Politi ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Cell Tracking ◽  
Superparamagnetic Iron Oxide ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Iron Oxide Particles ◽  
Superparamagnetic Iron Oxide Particles ◽  
Oxide Particles

scholarly journals Human inhalation exposure to iron oxide particles

2013 ◽  
Vol 14 (1-2) ◽  
pp. 5-23 ◽  
Author(s):  
Nastassja Lewinski ◽  
Halshka Graczyk ◽  
Michael Riediker
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Clinical Studies ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Inhalation Toxicity ◽  
Iron Oxide Particles ◽  
Oxide Particles

AbstractIn the past decade, many studies have been conducted to determine the health effects induced by exposure to engineered nanomaterials (NMs). Specifically for exposure via inhalation, numerous in vitro and animal in vivo inhalation toxicity studies on several types of NMs have been published. However, these results are not easily extrapolated to judge the effects of inhaling NMs in humans, and few published studies on the human response to inhalation of NMs exist. Given the emergence of more industries utilizing iron oxide nanoparticles as well as more nanomedicine applications of superparamagnetic iron oxide nanoparticles (SPIONs), this review presents an overview of the inhalation studies that have been conducted in humans on iron oxides. Both occupational exposure studies on complex iron oxide dusts and fumes, as well as human clinical studies on aerosolized, micron-size iron oxide particles are discussed. Iron oxide particles have not been described to elicit acute inhalation response nor promote lung disease after chronic exposure. The few human clinical studies comparing inhalation of fine and ultrafine metal oxide particles report no acute changes in the health parameters measured. Taken together existing evidence suggests that controlled human exposure to iron oxide nanoparticles, such as SPIONs, could be conducted safely.

Sentinel lymph node identification using superparamagnetic iron oxide particles versus radioisotope: The French Sentimag feasibility trial

2016 ◽  
Vol 113 (5) ◽  
pp. 501-507 ◽  
Author(s):  
Jean-Louis Houpeau ◽  
Marie-Pierre Chauvet ◽  
François Guillemin ◽  
Cécile Bendavid-Athias ◽  
Hélène Charitansky ◽  
...  
Keyword(s):  
Lymph Node ◽  
Iron Oxide ◽  
Sentinel Lymph Node ◽  
Superparamagnetic Iron Oxide ◽  
Feasibility Trial ◽  
Iron Oxide Particles ◽  
Sentinel Lymph Node Identification ◽  
Superparamagnetic Iron Oxide Particles ◽  
Oxide Particles

MR imaging of murine arthritis using ultrasmall superparamagnetic iron oxide particles☆

2001 ◽  
Vol 19 (9) ◽  
pp. 1209-1216 ◽  
Author(s):  
Bernard J Dardzinski ◽  
Vincent J Schmithorst ◽  
Scott K Holland ◽  
Gregory P Boivin ◽  
Tomoyuki Imagawa ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Mr Imaging ◽  
Superparamagnetic Iron Oxide ◽  
Iron Oxide Particles ◽  
Ultrasmall Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Particles ◽  
Oxide Particles

Efficient and Rapid Labeling of Transplanted Cell Populations with Superparamagnetic Iron Oxide Nanoparticles Using Cell Surface Chemical Biotinylation for in Vivo Monitoring by MRI

2010 ◽  
Vol 19 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Po-Wah So ◽  
Tammy Kalber ◽  
David Hunt ◽  
Michael Farquharson ◽  
Alia Al-Ebraheem ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Cell Tracking ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Cell Populations ◽  
Oxide Nanoparticles ◽  
Signal Loss

Determination of the dynamics of specific cell populations in vivo is essential for the development of cell-based therapies. For cell tracking by magnetic resonance imaging (MRI), cells need to internalize, or be surface labeled with a MRI contrast agent, such as superparamagnetic iron oxide nanoparticles (SPIOs): SPIOs give rise to signal loss by gradient-echo and T2-weighted MRI techniques. In this study, cancer cells were chemically tagged with biotin and then magnetically labeled with anti-biotin SPIOs. No significant detrimental effects on cell viability or death were observed following cell biotinylation. SPIO-labeled cells exhibited signal loss compared to non-SPIO-labeled cells by MRI in vitro. Consistent with the in vitro MRI data, signal attenuation was observed in vivo from SPIO-labeled cells injected into the muscle of the hind legs, or implanted subcutaneously into the flanks of mice, correlating with iron detection by histochemical and X-ray fluorescence (XRF) methods. To further validate this approach, human mesenchymal stem cells (hMSCs) were also employed. Chemical biotinylation and SPIO labeling of hMSCs were confirmed by fluorescence microscopy and flow cytometry. The procedure did not affect proliferation and multipotentiality, or lead to increased cell death. The SPIO-labeled hMSCs were shown to exhibit MRI signal reduction in vitro and was detectable in an in vivo model. In this study, we demonstrate a rapid, robust, and generic methodology that may be a useful and practical adjuvant to existing methods of cell labeling for in vivo monitoring by MRI. Further, we have shown the first application of XRF to provide iron maps to validate MRI data in SPIO-labeled cell tracking studies.

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