scholarly journals Expression of the p16INK4A Gene Is Associated Closely with Senescence of Human Mesenchymal Stem Cells and Is Potentially Silenced by DNA Methylation During In Vitro Expansion

Stem Cells ◽  
2007 ◽  
Vol 25 (9) ◽  
pp. 2371-2382 ◽  
Author(s):  
Kotaro R. Shibata ◽  
Tomoki Aoyama ◽  
Yasuko Shima ◽  
Kenichi Fukiage ◽  
Seiji Otsuka ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
P16ink4a Gene ◽  
In Vitro Expansion

scholarly journals Human Mesenchymal Stem Cells from Chorionic Villi and Amniotic Fluid are not Susceptible to Transformation after Extensive in Vitro Expansion

2011 ◽  
Vol 20 (5) ◽  
pp. 643-654 ◽  
Author(s):  
Antonella Poloni ◽  
Giulia Maurizi ◽  
Lucia Babini ◽  
Federica Serrani ◽  
Eleonora Berardinelli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Human Mesenchymal Stem Cells ◽  
Chorionic Villi ◽  
In Vitro Expansion

Correlation between in vitro expansion-related cell stiffening and differentiation potential of human mesenchymal stem cells

2015 ◽  
Vol 90 (1-3) ◽  
pp. 1-15 ◽  
Author(s):  
Courtney E. LeBlon ◽  
Meghan E. Casey ◽  
Caitlin R. Fodor ◽  
Tony Zhang ◽  
Xiaohui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Differentiation Potential ◽  
Related Cell ◽  
In Vitro Expansion

scholarly journals Nanochelating based nanocomplex, GFc7, improves quality and quantity of human mesenchymal stem cells during in vitro expansion

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Maryam Hafizi ◽  
Atena Hajarizadeh ◽  
Amir Atashi ◽  
Somayeh Kalanaky ◽  
Saideh Fakharzadeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Expansion

In Vitro Assessment of Hydroxyapatite Coating on the Surface of Additive Manufactured Ti6Al4V Scaffolds

2016 ◽  
Vol 879 ◽  
pp. 2444-2449 ◽  
Author(s):  
Ekaterina Chudinova ◽  
Maria Surmeneva ◽  
Andrey Koptioug ◽  
Irina V. Savintseva ◽  
Irina Selezneva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Nanocrystalline Structure ◽  
X Ray Diffraction ◽  
Ha Coating ◽  
Magnetron Sputter Deposition ◽  
In Vitro Assessment ◽  
Average Size

Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM®) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.

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