scholarly journals Three-Dimensional Perfusion Culture of Human Adipose Tissue-Derived Endothelial and Osteoblastic Progenitors Generates Osteogenic Constructs with Intrinsic Vascularization Capacity

Stem Cells ◽  
2007 ◽  
Vol 25 (7) ◽  
pp. 1823-1829 ◽  
Author(s):  
Arnaud Scherberich ◽  
Raffaele Galli ◽  
Claude Jaquiery ◽  
Jian Farhadi ◽  
Ivan Martin
Keyword(s):  
Adipose Tissue ◽  
Three Dimensional ◽  
Perfusion Culture ◽  
Human Adipose Tissue

scholarly journals Platelet Lysate as a Serum Substitute for 2D Static and 3D Perfusion Culture of Stromal Vascular Fraction Cells from Human Adipose Tissue

2009 ◽  
Vol 15 (4) ◽  
pp. 869-875 ◽  
Author(s):  
Andreas Marc Müller ◽  
Michael Davenport ◽  
Sophie Verrier ◽  
Raoul Droeser ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Perfusion Culture ◽  
Human Adipose Tissue ◽  
Platelet Lysate ◽  
Serum Substitute

scholarly journals Enhanced Regeneration of Vascularized Adipose Tissue with Dual 3D-Printed Elastic Polymer/dECM Hydrogel Complex

2021 ◽  
Vol 22 (6) ◽  
pp. 2886
Author(s):  
Soojin Lee ◽  
Hyun Su Lee ◽  
Justin J. Chung ◽  
Soo Hyun Kim ◽  
Jong Woong Park ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Adipogenic Differentiation ◽  
Three Dimensional ◽  
Human Adipose Tissue ◽  
Collagen Type I ◽  
Type I ◽  
Adipose Tissue Engineering ◽  
Decellularized Extracellular Matrix ◽  
In Vivo Experiments

A flexible and bioactive scaffold for adipose tissue engineering was fabricated and evaluated by dual nozzle three-dimensional printing. A highly elastic poly (L-lactide-co-ε-caprolactone) (PLCL) copolymer, which acted as the main scaffolding, and human adipose tissue derived decellularized extracellular matrix (dECM) hydrogels were used as the printing inks to form the scaffolds. To prepare the three-dimensional (3D) scaffolds, the PLCL co-polymer was printed with a hot melting extruder system while retaining its physical character, similar to adipose tissue, which is beneficial for regeneration. Moreover, to promote adipogenic differentiation and angiogenesis, adipose tissue-derived dECM was used. To optimize the printability of the hydrogel inks, a mixture of collagen type I and dECM hydrogels was used. Furthermore, we examined the adipose tissue formation and angiogenesis of the PLCL/dECM complex scaffold. From in vivo experiments, it was observed that the matured adipose-like tissue structures were abundant, and the number of matured capillaries was remarkably higher in the hydrogel–PLCL group than in the PLCL-only group. Moreover, a higher expression of M2 macrophages, which are known to be involved in the remodeling and regeneration of tissues, was detected in the hydrogel–PLCL group by immunofluorescence analysis. Based on these results, we suggest that our PLCL/dECM fabricated by a dual 3D printing system will be useful for the treatment of large volume fat tissue regeneration.

scholarly journals Lymphatic vessels in human adipose tissue

2019 ◽  
Vol 379 (3) ◽  
pp. 511-520 ◽  
Author(s):  
Patricia de Albuquerque Garcia Redondo ◽  
Fernanda Gubert ◽  
Camila Zaverucha-do-Valle ◽  
Tatiana Pereira Pena Dutra ◽  
Jackline de Paula Ayres-Silva ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Three Dimensional ◽  
Lymphatic Vessels ◽  
Human Adipose Tissue ◽  
Unanswered Question ◽  
Three Dimensional Imaging ◽  
Whole Mount ◽  
Lymphatic Network ◽  
Vascular Structures

AbstractDespite being considered present in most vascularised tissues, lymphatic vessels have not been properly shown in human adipose tissue (AT). Our goal in this study is to investigate an unanswered question in AT biology, regarding lymphatic network presence in tissue parenchyma. Using human subcutaneous (S-) and visceral (V-) AT samples with whole mount staining for lymphatic specific markers and three-dimensional imaging, we showed lymphatic capillaries and larger lymphatic vessels in the human VAT. Conversely, in the human SAT, microcirculatory lymphatic vascular structures were rarely detected and no initial lymphatics were found.

Development of hepatic blocks using human adipose tissue-derived stem cells through three-dimensional cell printing techniques

2017 ◽  
Vol 5 (5) ◽  
pp. 1098-1107 ◽  
Author(s):  
Ji-Seon Lee ◽  
Hyeon Yoon ◽  
Dajeong Yoon ◽  
Geun Hyung Kim ◽  
Hyeong Tae Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Liver Transplantation ◽  
Adipose Tissue ◽  
Liver Diseases ◽  
Three Dimensional ◽  
Human Adipose Tissue ◽  
Cell Printing ◽  
Printing Techniques ◽  
Dimensional Cell

Currently, most acute liver diseases are treated through liver transplantation.

scholarly journals Sustainable Three-Dimensional Tissue Model of Human Adipose Tissue

2013 ◽  
Vol 19 (10) ◽  
pp. 745-754 ◽  
Author(s):  
Evangelia Bellas ◽  
Kacey G. Marra ◽  
David L. Kaplan
Keyword(s):  
Adipose Tissue ◽  
Three Dimensional ◽  
Human Adipose Tissue ◽  
Tissue Model
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