scholarly journals Efficient Derivation of Embryonic Stem Cells by Inhibition of Glycogen Synthase Kinase-3

Stem Cells ◽  
2007 ◽  
Vol 25 (11) ◽  
pp. 2705-2711 ◽  
Author(s):  
Hiroki Umehara ◽  
Tohru Kimura ◽  
Satoshi Ohtsuka ◽  
Toshinobu Nakamura ◽  
Kenji Kitajima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glycogen Synthase ◽  
Embryonic Stem ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3

scholarly journals Glycogen synthase kinase-3 (GSK-3) activity regulates mRNA methylation in mouse embryonic stem cells

2018 ◽  
Vol 293 (27) ◽  
pp. 10731-10743 ◽  
Author(s):  
Kelsie J. Faulds ◽  
Jennifer N. Egelston ◽  
Laura J. Sedivy ◽  
Matthew K. Mitchell ◽  
Sanjana Garimella ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glycogen Synthase ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3 ◽  
Mrna Methylation

scholarly journals Glycogen synthase kinase-3 (Gsk-3) plays a fundamental role in maintaining DNA methylation at imprinted loci in mouse embryonic stem cells

2015 ◽  
Vol 26 (11) ◽  
pp. 2139-2150 ◽  
Author(s):  
Gavin D. Meredith ◽  
Anthony D'Ippolito ◽  
Miroslav Dudas ◽  
Leigh C. Zeidner ◽  
Logan Hostetter ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Dna Methylation ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glycogen Synthase ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3 ◽  
Gsk 3Β

Glycogen synthase kinase-3 (Gsk-3) is a key regulator of multiple signal transduction pathways. Recently we described a novel role for Gsk-3 in the regulation of DNA methylation at imprinted loci in mouse embryonic stem cells (ESCs), suggesting that epigenetic changes regulated by Gsk-3 are likely an unrecognized facet of Gsk-3 signaling. Here we extend our initial observation to the entire mouse genome by enriching for methylated DNA with the MethylMiner kit and performing next-generation sequencing (MBD-Seq) in wild-type and Gsk-3α−/−;Gsk-3β−/− ESCs. Consistent with our previous data, we found that 77% of known imprinted loci have reduced DNA methylation in Gsk-3-deficient ESCs. More specifically, we unambiguously identified changes in DNA methylation within regions that have been confirmed to function as imprinting control regions. In many cases, the reduced DNA methylation at imprinted loci in Gsk-3α−/−;Gsk-3β−/− ESCs was accompanied by changes in gene expression as well. Furthermore, many of the Gsk-3–dependent, differentially methylated regions (DMRs) are identical to the DMRs recently identified in uniparental ESCs. Our data demonstrate the importance of Gsk-3 activity in the maintenance of DNA methylation at a majority of the imprinted loci in ESCs and emphasize the importance of Gsk-3–mediated signal transduction in the epigenome.

scholarly journals Glycogen Synthase Kinase 3 (GSK3) Inhibitor, SB-216763, Promotes Pluripotency in Mouse Embryonic Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39329 ◽  
Author(s):  
Leslie A. Kirby ◽  
Jason T. Schott ◽  
Brenda L. Noble ◽  
Daniel C. Mendez ◽  
Paul S. Caseley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Glycogen Synthase ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Glycogen Synthase Kinase ◽  
Glycogen Synthase Kinase 3 ◽  
Sb 216763 ◽  
Gsk3 Inhibitor
Sign in / Sign up

Export Citation Format

Share Document