Essential Alterations of Heparan Sulfate During the Differentiation of Embryonic Stem Cells to Sox1-Enhanced Green Fluorescent Protein-Expressing Neural Progenitor Cells

Stem Cells ◽  
2007 ◽  
Vol 25 (9) ◽  
pp. 2389-2389
Author(s):  
Claire E. Johnson ◽  
Brett E. Crawford ◽  
Marios Stavridis ◽  
Gerdy ten Dam ◽  
Annie L. Wat ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Heparan Sulfate ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

Maintenance of Pluripotency in Human Embryonic Stem Cells Stably Over-expressing Enhanced Green Fluorescent Protein

2004 ◽  
Vol 13 (6) ◽  
pp. 636-645 ◽  
Author(s):  
Yi-Ping Liu ◽  
Oksana V. Dovzhenko ◽  
Mark A. Garthwaite ◽  
Svetlana V. Dambaeva ◽  
Maureen Durning ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells

2006 ◽  
pp. 305-312
Author(s):  
Yutaka Suzuki ◽  
Tatsuyuki Takada ◽  
Nae Kadota ◽  
Yasushi Kondo ◽  
Ryuzo Torii
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

Survival and differentiation of neural progenitor cells derived from embryonic stem cells and transplanted into ischemic brain

2005 ◽  
Vol 103 (2) ◽  
pp. 304-310 ◽  
Author(s):  
Yasushi Takagi ◽  
Masaki Nishimura ◽  
Asuka Morizane ◽  
Jun Takahashi ◽  
Kazuhiko Nozaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Ischemic Brain ◽  
Content Type ◽  
Fine Print

Object. Cell replacement therapy including the use of embryonic stem cells (ESCs) may represent a novel treatment for damage from stroke. In this study, the authors transplanted neural progenitor cells (NPCs) derived from ESCs into ischemic brain and analyzed their survival and differentiation. Methods. Multipotential NPCs were generated from ESCs by using the stromal cell—derived inducing activity method. These cells could differentiate in vitro into neurons, glia, and oligodendrocytes, thus revealing them to be neural stem cells. The NPCs were then transplanted into ischemic brain. At 2 weeks postischemia, the transplanted cells occupied 18.8 ± 2.5% of the hemispheric area; by 4 weeks postischemia, 26.5 ± 4% of the hemisphere. At 4 weeks after transplantation, green fluorescent protein (GFP)—positive transplanted cells showed mature neuronal morphological features. The authors also investigated the expression of differentiation markers and various neurotransmitters. Transplanted cells were immunopositive for neuronal nuclei, β-tubulin-III, and glial fibrillary acidic protein. Of the GFP-positive cells, 33.3 ± 11.5% were positive for glutamate decarboxylase, 13.3 ± 5.8% for glutamate, 2.1 ± 2.5% for tyrosine hydroxylase, 1.8 ± 2% for serotonin, and 0.4 ± 0.2% for choline acetyltransferase. Conclusions. The authors confirmed the survival and differentiation of ESC-derived NPCs transplanted into the ischemic brain. Surviving transplanted cells expressed several neural markers and neurotransmitters. These findings indicate that these cells can function in the brain.

scholarly journals Embryonic Stem Cells and Transgenic Mice Ubiquitously Expressing a Tau-Tagged Green Fluorescent Protein

2000 ◽  
Vol 228 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Thomas Pratt ◽  
Linda Sharp ◽  
Jenny Nichols ◽  
David J. Price ◽  
John O. Mason
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

scholarly journals In Vivo and In Vitro Tissue-Specific Expression of Green Fluorescent Protein Using the Cre-Lox System in Mouse Embryonic Stem Cells

Stem Cells ◽  
2005 ◽  
Vol 23 (1) ◽  
pp. 10-15 ◽  
Author(s):  
Jan Schindehütte ◽  
Hidefumi Fukumitsu ◽  
Patrick Collombat ◽  
Gundula Griesel ◽  
Christopher Brink ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Specific Expression ◽  
Tissue Specific Expression ◽  
Green Fluorescent
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