scholarly journals Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer

Stem Cells ◽  
2006 ◽  
Vol 25 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Takafusa Hikichi ◽  
Sayaka Wakayama ◽  
Eiji Mizutani ◽  
Yasuhiro Takashima ◽  
Satoshi Kishigami ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Differentiation Potential

scholarly journals Defective chromatin architectures in embryonic stem cells derived from somatic cell nuclear transfer impair their differentiation potentials

2021 ◽  
Vol 12 (12) ◽  
Author(s):  
Dan-Ya Wu ◽  
Xinxin Li ◽  
Qiao-Ran Sun ◽  
Cheng-Li Dou ◽  
Tian Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Genome Organization ◽  
Nuclear Transfer ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Chromatin Accessibility ◽  
Differentiation Potential ◽  
3D Genome

AbstractNuclear transfer embryonic stem cells (ntESCs) hold enormous promise for individual-specific regenerative medicine. However, the chromatin states of ntESCs remain poorly characterized. In this study, we employed ATAC-seq and Hi-C techniques to explore the chromatin accessibility and three-dimensional (3D) genome organization of ntESCs. The results show that the chromatin accessibility and genome structures of somatic cells are re-arranged to ESC-like states overall in ntESCs, including compartments, topologically associating domains (TADs) and chromatin loops. However, compared to fertilized ESCs (fESCs), ntESCs show some abnormal openness and structures that have not been reprogrammed completely, which impair the differentiation potential of ntESCs. The histone modification H3K9me3 may be involved in abnormal structures in ntESCs, including incorrect compartment switches and incomplete TAD rebuilding. Moreover, ntESCs and iPSCs show high similarity in 3D genome structures, while a few differences are detected due to different somatic cell origins and reprogramming mechanisms. Through systematic analyses, our study provides a global view of chromatin accessibility and 3D genome organization in ntESCs, which can further facilitate the understanding of the similarities and differences between ntESCs and fESCs.

Lymphoid lineage differentiation potential of mouse nuclear transfer embryonic stem cells

Biologicals ◽  
2015 ◽  
Vol 43 (5) ◽  
pp. 349-354 ◽  
Author(s):  
Tarlan Eslami-Arshaghi ◽  
Mohammad Salehi ◽  
Masoud Soleimani ◽  
Mazaher Gholipourmalekabadi ◽  
Majid Mossahebi-Mohammadi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Differentiation Potential ◽  
Lymphoid Lineage ◽  
Lineage Differentiation

scholarly journals Downregulation of H19 Improves the Differentiation Potential of Mouse Parthenogenetic Embryonic Stem Cells

2012 ◽  
Vol 21 (7) ◽  
pp. 1134-1144 ◽  
Author(s):  
Neli P. Ragina ◽  
Karianne Schlosser ◽  
Jason G. Knott ◽  
Patricia K. Senagore ◽  
Pamela J. Swiatek ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Differentiation Potential

A Novel Method for Somatic Cell Nuclear Transfer to Mouse Embryonic Stem Cells

2005 ◽  
Vol 7 (4) ◽  
pp. 265-271 ◽  
Author(s):  
Danièle Pralong ◽  
Krzysztof Mrozik ◽  
Filomena Occhiodoro ◽  
Nishanthi Wijesundara ◽  
Huseyin Sumer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Novel Method

scholarly journals Wnt Signaling Regulates the Lineage Differentiation Potential of Mouse Embryonic Stem Cells through Tcf3 Down-Regulation

PLoS Genetics ◽  
2013 ◽  
Vol 9 (5) ◽  
pp. e1003424 ◽  
Author(s):  
Yaser Atlasi ◽  
Rubina Noori ◽  
Claudia Gaspar ◽  
Patrick Franken ◽  
Andrea Sacchetti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Wnt Signaling ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Down Regulation ◽  
Differentiation Potential ◽  
Lineage Differentiation
Sign in / Sign up

Export Citation Format

Share Document