scholarly journals A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single-Cell Dissociation

Stem Cells ◽  
2006 ◽  
Vol 24 (12) ◽  
pp. 2649-2660 ◽  
Author(s):  
Kouichi Hasegawa ◽  
Tsuyoshi Fujioka ◽  
Yukio Nakamura ◽  
Norio Nakatsuji ◽  
Hirofumi Suemori
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Single Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell Dissociation ◽  
Selection Of

scholarly journals Lineage Selection of Functional and Cryopreservable Human Embryonic Stem Cell-Derived Neurons

Stem Cells ◽  
2008 ◽  
Vol 26 (7) ◽  
pp. 1705-1712 ◽  
Author(s):  
Julia Ladewig ◽  
Philipp Koch ◽  
Elmar Endl ◽  
Banu Meiners ◽  
Thoralf Opitz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Selection Of

scholarly journals Single Cell RNA Sequencing of stem cell-derived retinal ganglion cells

2017 ◽  
Author(s):  
Maciej Daniszewski ◽  
Anne Senabouth ◽  
Quan Nguyen ◽  
Duncan E. Crombie ◽  
Samuel W. Lukowski ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Single Cell ◽  
Rna Sequencing ◽  
Retinal Ganglion Cells ◽  
Human Embryonic Stem Cell ◽  
Ganglion Cells ◽  
Embryonic Stem ◽  
Retinal Ganglion ◽  
Single Cell Rna Sequencing

ABSTRACTWe used human embryonic stem cell-derived retinal ganglion cells (RGCs) to characterize the transcriptome of 1,174 cells at the single cell level. The human embryonic stem cell line BRN3B-mCherry A81-H7 was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and subsequently prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells upregulated genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature phenotype.

Identification and selection of cardiomyocytes during human embryonic stem cell differentiation

2007 ◽  
Vol 21 (10) ◽  
pp. 2551-2563 ◽  
Author(s):  
Irit Huber ◽  
Ilanit Itzhaki ◽  
Oren Caspi ◽  
Gil Arbel ◽  
Maty Tzukerman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Selection Of

Identification of Cell Surface Proteins for Antibody-Based Selection of Human Embryonic Stem Cell-Derived Cardiomyocytes

2010 ◽  
Vol 9 (3) ◽  
pp. 1610-1618 ◽  
Author(s):  
Dennis Van Hoof ◽  
Wilma Dormeyer ◽  
Stefan R. Braam ◽  
Robert Passier ◽  
Jantine Monshouwer-Kloots ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Surface ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Surface Proteins ◽  
Cell Surface Proteins ◽  
Selection Of
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