Glucocorticoids Promote Chondrogenic Differentiation of Adult Human Mesenchymal Stem Cells by Enhancing Expression of Cartilage Extracellular Matrix Genes

Stem Cells ◽  
2006 ◽  
Vol 24 (6) ◽  
pp. 1487-1495 ◽  
Author(s):  
Assia Derfoul ◽  
Geraldine L. Perkins ◽  
David J. Hall ◽  
Rocky S. Tuan
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Adult Human ◽  
Cartilage Extracellular Matrix

Annexin II Mediates Plasminogen-Dependant Matrix Invasion by Adult Human Mesenchymal Stem Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2326-2326
Author(s):  
Paul B. Bolno ◽  
Doris A. Morgan ◽  
Mahesh Sharma ◽  
Martin Lazorik ◽  
Andrew S. Wechsler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Western Blot ◽  
Human Mesenchymal Stem Cells ◽  
Angiogenic Factors ◽  
Annexin Ii ◽  
Adult Human

Abstract Background: Annexin II (ANX2) is a fibrinolytic receptor that serves as a binding site for plasminogen and tissue plasminogen activator, facilitating the generation of plasmin. ANX2 is present on a wide variety of cells including vascular endothelial cells as well as macrophages. ANX2 has been shown to play a key role in extracellular matrix degradation, cellular migration, and invasion. This degradation of extracellular matrix may also cause the release of matrix-bound angiogenic factors such as VEGF and FGF. We hypothesized that adult human mesenchymal stem cells (hMSCs) express ANX2 and utilize this receptor for plasmin generation to facilitate basement membrane invasion. Methods: Primary hMSCs were isolated from the sternal bone marrow of patients undergoing median sternotomy. Stem cell surface markers were characterized via immuno-fluorescence. The presence of ANX2 protein by hMSCs was established via western blot. ANX2 mediated plasminogen activation and plasmin generation was quantified using chromozyme-P as a colorimetric substrate. Invasion assays were performed in dual-chamber culture wells containing matrigel inserts. hMSCs were plated into upper chambers containing: serum-free medium (SFM), SFM + Plasminogen, or SFM + Plasminogen + epsilon-aminocaproic acid (e-ACA inhibits binding of plasminogen to ANX2). After 24 hours, invasive cells were isolated and counted. Results: Sternal bone marrow derived hMSCs expressed the membrane phenotype CD34 (−), CD14 (−), CD44 (+), CD105 (+), CD106 (+). The presence of ANX2 was confirmed by western blot analysis. hMSCs generated 1.95 units of plasmin per milligram of protein. There was a 20% (p 0 .004) increase in hMSC invasion in the wells containing plasminogen as compared to SFM alone. When e-ACA was introduced there was a decrease in hMSC invasion back to control values. Conclusion: Our observations establish for the first time the presence and functional activity of ANX2 in hMSCs. These data suggest that mesenchymal stem cell expression of ANX2 facilitates plasminogen-mediated hMSC trans-endothelial invasion, migration and the release of pro-angiogenic factors from within the extracellular matrix, promoting stem cell directed repair and angiogenesis.

The effect of cartilage extracellular matrix particle size on the chondrogenic differentiation of bone marrow mesenchymal stem cells

2019 ◽  
Vol 14 (7) ◽  
pp. 663-680 ◽  
Author(s):  
Chenjun Zhai ◽  
Xiao Zhang ◽  
Jun Chen ◽  
Jian He ◽  
Hao Fei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Particle Size ◽  
Mesenchymal Stem Cells ◽  
Small Particle ◽  
Large Particle ◽  
Chondrogenic Differentiation ◽  
Glycolic Acid ◽  
Cartilage Extracellular Matrix

Aim: To investigate the effect of cartilage extracellular matrix (ECM) particle size on the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). Materials & methods: BMSCs were seeded into the scaffolds fabricated by small particle ECM materials and large particle ECM materials. For the positive control, chondrogenically induced BMSCs were seeded into commercial poly-lactic-glycolic acid scaffolds. Macroscopic observation, histological and immunohistochemical staining, mechanical testing and biochemical analysis were performed to the cell-scaffold constructs. Results: BMSCs in small particle ECM materials and poly-lactic-glycolic acid scaffolds were induced to differentiate into chondrocytes, while BMSCs in the large particle ECM materials scaffold did not differentiate into chondrocytes. Conclusion: The small ECM particle materials improved the induction ability of the cartilage ECM-derived scaffold.

scholarly journals Laminin-5 activates extracellular matrix production and osteogenic gene focusing in human mesenchymal stem cells

2007 ◽  
Vol 26 (2) ◽  
pp. 106-114 ◽  
Author(s):  
Robert F. Klees ◽  
Roman M. Salasznyk ◽  
Scott Vandenberg ◽  
Kristin Bennett ◽  
George E. Plopper
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Laminin 5 ◽  
Extracellular Matrix Production ◽  
Matrix Production ◽  
Osteogenic Gene

Biomimetic hydrogels for chondrogenic differentiation of human mesenchymal stem cells to neocartilage

Biomaterials ◽  
2010 ◽  
Vol 31 (28) ◽  
pp. 7298-7307 ◽  
Author(s):  
Shao Qiong Liu ◽  
Quan Tian ◽  
James L. Hedrick ◽  
James Hoi Po Hui ◽  
Pui Lai Rachel Ee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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