HumanERasGene Has an Upstream Premature Polyadenylation Signal That Results in a Truncated, Noncoding Transcript

Stem Cells ◽  
2005 ◽  
Vol 23 (10) ◽  
pp. 1535-1540 ◽  
Author(s):  
Takashi Kameda ◽  
James A. Thomson
Keyword(s):  
Polyadenylation Signal ◽  
Noncoding Transcript

scholarly journals Expression of ferrochelatase mRNA in erythroid and non-erythroid cells

1993 ◽  
Vol 292 (2) ◽  
pp. 343-349 ◽  
Author(s):  
R Y Y Chan ◽  
H M Schulman ◽  
P Ponka
Keyword(s):  
Sequence Similarity ◽  
Erythroid Differentiation ◽  
Polyadenylation Signal ◽  
Erythroid Cell ◽  
Erythroid Cells ◽  
Erythroleukemia Cells ◽  
Haem Biosynthesis ◽  
Polyadenylation Sites ◽  
Dna Fragment ◽  
Polyadenylation Signals

Ferrochelatase, which catalyses the last step in haem biosynthesis, i.e. the insertion of Fe(II) into protophorphyrin IX, is present in all cells, but is particularly abundant in erythroid cells during haemoglobinization. Using mouse ferrochelatase cDNA as a probe two ferrochelatase transcripts, having lengths of 2.9 kb and 2.2 kb, were found in extracts of mouse liver, kidney, brain, muscle and spleen, the 2.9 kb transcript being more abundant in the non-erythroid tissues and the 2.2 kb transcript more predominant in spleen. In mouse erythroleukemia cells the 2.9 kb ferrochelatase transcript is also more abundant; however, following induction of erythroid differentiation by dimethyl sulphoxide there is a preferential increase in the 2.2 kb transcript, which eventually predominates. With mouse reticulocytes, the purest immature erythroid cell population available, over 90% of the total ferrochelatase mRNA is present as the 2.2 kb transcript. Since there is probably only one mouse ferrochelatase gene, the occurrence of two ferrochelatase transcripts could arise from the use of two putative polyadenylation signals in the 3′ region of ferrochelatase DNA. This possibility was explored by using a 389 bp DNA fragment produced by PCR with synthetic oligoprimers having sequence similarity with a region between the polyadenylation sites. This fragment hybridized only to the 2.9 kb ferrochelatase transcript, indicating that the two transcripts differ at their 3′ ends and suggesting that the 2.2 kb transcript results from the utilization of the upstream polyadenylation signal. The preferential utilization of the upstream polyadenylation signal may be an erythroid-specific characteristic of ferrochelatase gene expression.

scholarly journals The polyadenylation site mutation in the alpha-globin gene cluster

Blood ◽  
1988 ◽  
Vol 71 (2) ◽  
pp. 313-319 ◽  
Author(s):  
SL Thein ◽  
RB Wallace ◽  
L Pressley ◽  
JB Clegg ◽  
DJ Weatherall ◽  
...  
Keyword(s):  
Middle Eastern ◽  
Globin Gene ◽  
Saudi Arabian ◽  
Polyadenylation Signal ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Site Mutation ◽  
Mediterranean Populations ◽  
Alpha Globin ◽  
Hb H Disease

In a previous study, we described a form of nondeletion alpha- thalassemia (alpha T Saudi alpha) found in subjects of Saudi Arabian origin. In the current study, using synthetic oligoprobe hybridization and restriction enzyme analysis, we have demonstrated that the molecular basis of alpha T Saudi alpha is due solely to a single base mutation (AATAAA----AATAAG) in the polyadenylation signal of the alpha 2 gene and that the frameshift mutation in codon 14 of the linked alpha 1 gene is the result of a cloning artefact. The alpha 2 polyadenylation signal mutation occurs in other Middle Eastern and the Mediterranean populations and is responsible for the clinical phenotype of Hb H disease in some Saudi Arabian individuals with five alpha genes (alpha T Saudi alpha/(alpha alpha alpha)T Saudi). Evidence suggests that the (alpha alpha alpha)T Saudi haplotype has arisen as a result of a recombination between two misaligned chromosomes bearing the alpha T Saudi alpha defect.

scholarly journals A novel TP53 variant (rs78378222 A > C) in the polyadenylation signal is associated with increased cancer susceptibility: evidence from a meta-analysis

Oncotarget ◽  
2016 ◽  
Vol 7 (22) ◽  
pp. 32854-32865 ◽  
Author(s):  
Ying Wang ◽  
Xue-Song Wu ◽  
Jing He ◽  
Tianjiao Ma ◽  
Wei Lei ◽  
...  
Keyword(s):  
Cancer Susceptibility ◽  
Meta Analysis ◽  
Polyadenylation Signal

Targeting IgE polyadenylation signal with antisense oligonucleotides decreases IgE secretion and plasma cell viability

2021 ◽  
Author(s):  
Anne Marchalot ◽  
Catherine Horiot ◽  
Jean-Marie Lambert ◽  
Claire Carrion ◽  
Christelle Oblet ◽  
...  
Keyword(s):  
Cell Viability ◽  
Plasma Cell ◽  
Antisense Oligonucleotides ◽  
Polyadenylation Signal

A genomic clone encoding a novel proliferation-dependent histone H2A.1 mRNA enriched in the poly(A)+ fraction

1990 ◽  
Vol 10 (6) ◽  
pp. 2848-2854
Author(s):  
L Fecker ◽  
P Ekblom ◽  
M Kurkinen ◽  
M Ekblom
Keyword(s):  
Genomic Sequence ◽  
Genomic Clone ◽  
Polyadenylation Signal ◽  
Histone H2a ◽  
Hairpin Loop ◽  
Coding Region ◽  
Genomic Clones ◽  
Histone Mrnas ◽  
Cellulose Column

Replication-dependent histone mRNAs are prime examples of nonpolyadenylated mRNAs. We isolated and characterized cDNAs and a genomic clone for a replication-dependent histone H2A.1 mRNA which segregated into the poly(A)+ fraction during mRNA isolation through an oligo(dT)-cellulose column. However, the results of sequencing of the genomic clone suggested that the mRNA did not contain a poly(A) tail. Instead, the genomic sequence revealed a nonterminal oligo(A) tract directly upstream from the typical 3'-terminal hairpin loop of replication-dependent histone mRNAs. The nonterminal oligo(A) tract consisted of 14 adenylate residues interrupted by one guanylate residue (A4GA10). We concluded that this short oligo(A) stretch mediated binding of the mRNA to oligo(dT) even after stringent washes with 0.1 M NaCl, indicating that rather short oligo(A) sequences can ensure binding to oligo(dT)-cellulose. The cDNA and genomic clones contained an AAATAAG sequence at the end of the coding region. It has been suggested that this sequence contains a polyadenylation signal in some yeast and mouse transcripts, but it does not function as a polyadenylation signal in the histone transcript described in this paper.

Growth-dependent expression of dihydrofolate reductase mRNA from modular cDNA genes

1983 ◽  
Vol 3 (9) ◽  
pp. 1598-1608
Author(s):  
R J Kaufman ◽  
P A Sharp
Keyword(s):  
Dihydrofolate Reductase ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Polyadenylation Signal ◽  
Ovary Cells ◽  
Dividing Cells ◽  
Major Late Promoter ◽  
The Stability

Dihydrofolate reductase (DHFR) synthesis is regulated in a growth-dependent fashion. Dividing cells synthesize DHFR at a 10-fold-higher rate than do stationary cells. To study this growth-dependent synthesis. DHFR genes have been constructed from a DHFR cDNA segment, the adenovirus major late promoter, and fragments of simian virus 40 (SV40) which provide signals for polyadenylation. These genes have been introduced into Chinese hamster ovary cells. The DHFR mRNAs produced in different transformants are identical at their 5' ends, but differ in sequences in their 3' ends as different sites are utilized for polyadenylation. Three transformants that utilize either DHFR polyadenylation signals or the SV40 late polyadenylation signal exhibit growth-dependent DHFR synthesis. The level of DHFR mRNA in growing cells is approximately 10 times that in stationary cells for these transformants. This growth-dependent DHFR mRNA production probably results from posttranscriptional events. In contrast, three transformants that utilize the SV40 early polyadenylation signal and another transformant that utilizes a cellular polyadenylation signal do not exhibit growth-dependent DHFR synthesis. In these three cell lines, the fraction of mRNAs polyadenylated at different sites in a tandem array shifts between growing and stationary cells. These results suggest that the metabolic state of the cell is important in determining either the efficiency of polyadenylation at various sites or the stability of mRNA polyadenylated at various sites.

scholarly journals A Composite Polyadenylation Signal with TATA Box Function

2000 ◽  
Vol 20 (3) ◽  
pp. 834-841 ◽  
Author(s):  
Nir Paran ◽  
Assaf Ori ◽  
Izhak Haviv ◽  
Yosef Shaul
Keyword(s):  
Viral Genome ◽  
Transcription Initiation ◽  
Reporter Genes ◽  
Polyadenylation Signal ◽  
Tata Box ◽  
Dual Function ◽  
Reverse Transcriptase Gene ◽  
Hbv Replication ◽  
Viral Mutant ◽  
B Virus

ABSTRACT A variant polyadenylation signal, which is conserved and employed by mammalian hepadnaviruses, has a sequence resembling that of the TATA box. We report here that this composite box manifests all the promoter characteristics. It binds effectively TATA-binding protein with TFIIB and TFIIA in a synergistic manner. This capacity, however, is lost when the box is converted to a canonical and simple poly(A) signal. Furthermore, we show that it has promoter activity and supports transcription of reporter genes preferentially in liver-derived cells, a characteristic behavior of the hepatitis B virus (HBV) promoters. In addition, we show that the HBV noncanonical poly(A) signal supports transcription initiation from the viral genome, suggesting that it is a genuine promoter, possibly of the polymerase/reverse transcriptase gene. Finally, we found that this deviant poly(A) signal is crucial for HBV replication since a viral mutant with a canonical poly(A) box is impaired in replication. Our data, therefore, raise the interesting and novel possibility that a composite poly(A) box might have a dual function. At the level of DNA it functions as a promoter to initiate transcription, whereas at the level of RNA it serves as a poly(A) signal to process RNA. An interesting outcome of this strategy of gene expression is that it provides a novel mechanism for the synthesis of an approximately genome length transcript.

scholarly journals Activation of LDL Receptor Expression by Small RNAs Complementary to a Noncoding Transcript that Overlaps the LDLR Promoter

2010 ◽  
Vol 17 (12) ◽  
pp. 1344-1355 ◽  
Author(s):  
Masayuki Matsui ◽  
Fuminori Sakurai ◽  
Sayda Elbashir ◽  
Donald J. Foster ◽  
Muthiah Manoharan ◽  
...  
Keyword(s):  
Small Rnas ◽  
Ldl Receptor ◽  
Receptor Expression ◽  
Noncoding Transcript
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