scholarly journals High-Efficiency RNA Interference in Human Embryonic Stem Cells

Stem Cells ◽  
2005 ◽  
Vol 23 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Holm Zaehres ◽  
M. William Lensch ◽  
Laurence Daheron ◽  
Sheila A. Stewart ◽  
Joseph Itskovitz-Eldor ◽  
...  
Keyword(s):  
Stem Cells ◽  
Rna Interference ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
High Efficiency ◽  
Embryonic Stem

scholarly journals Nucleofection Mediates High-Efficiency Stable Gene Knockdown and Transgene Expression in Human Embryonic Stem Cells

Stem Cells ◽  
2008 ◽  
Vol 26 (6) ◽  
pp. 1436-1443 ◽  
Author(s):  
Kristi A. Hohenstein ◽  
April D. Pyle ◽  
Jing Yi Chern ◽  
Leslie F. Lock ◽  
Peter J. Donovan
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Transgene Expression ◽  
High Efficiency ◽  
Embryonic Stem ◽  
Gene Knockdown ◽  
Stable Gene

scholarly journals Specific Knockdown of OCT4 in Human Embryonic Stem Cells by Inducible Short Hairpin RNA Interference

Stem Cells ◽  
2009 ◽  
Vol 27 (4) ◽  
pp. 776-782 ◽  
Author(s):  
Gaetano Zafarana ◽  
Stuart R. Avery ◽  
Katie Avery ◽  
Harry D. Moore ◽  
Peter W. Andrews
Keyword(s):  
Stem Cells ◽  
Rna Interference ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Short Hairpin Rna ◽  
Hairpin Rna ◽  
Short Hairpin ◽  
Short Hairpin Rna Interference

The derivation and potential use of human embryonic stem cells

2001 ◽  
Vol 13 (8) ◽  
pp. 523 ◽  
Author(s):  
Alan O. Trounson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Regenerative Medicine ◽  
Human Embryonic Stem Cells ◽  
High Efficiency ◽  
Es Cells ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Cell Types ◽  
Specific Cell

Human embryonic stem cells lines can be derived from human blastocysts at high efficiency (>50%) by immunosurgical isolation of the inner cell mass and culture on embryonic fibroblast cell lines. These cells will spontaneously differentiate into all the primary embryonic lineages in vitro and in vivo, but they are unable to form an integrated embryo or body plan by themselves or when combined with trophectoderm cells. They may be directed into a number of specific cell types and this enrichment process requires specific growth factors, cell-surface molecules, matrix molecules and secreted products of other cell types. Embryonic stem (ES) cells are immortal and represent a major potential for cell therapies for regenerative medicine. Their use in transplantation may depend on the formation of a large bank of suitable human leucocyte antigen (HLA) types or the genetic erasure of their HLA expression. Successful transplantation may also require induction of tolerance in recipients and ongoing immune suppression. Although it is possible to customize ES cells by therapeutic cloning or cytoplasmic transfer, it would appear unlikely that these strategies will be used extensively for producing ES cells compatible for transplantation. Embryonic stem cell research may deliver a new pathway for regenerative medicine.

scholarly journals High-efficiency siRNA-based gene knockdown in human embryonic stem cells

RNA ◽  
2010 ◽  
Vol 16 (12) ◽  
pp. 2564-2569 ◽  
Author(s):  
Y. Ma ◽  
J. Jin ◽  
C. Dong ◽  
E.-C. Cheng ◽  
H. Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
High Efficiency ◽  
Embryonic Stem ◽  
Gene Knockdown
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