scholarly journals Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527

2019 ◽  
Vol 20 (11) ◽  
pp. 891-900 ◽  
Author(s):  
Zhang-qing Song ◽  
Zhi-jun Liao ◽  
Ye-feng Hu ◽  
Zheng Ma ◽  
Andreas Bechthold ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Streptomyces Rimosus ◽  
Conjugation System ◽  
Intergeneric Conjugation

scholarly journals Negative Feedback Regulation of RIG-I-Mediated Antiviral Signaling by Interferon-Induced ISG15 Conjugation

2007 ◽  
Vol 82 (3) ◽  
pp. 1474-1483 ◽  
Author(s):  
Min-Jung Kim ◽  
Sun-Young Hwang ◽  
Tadaatsu Imaizumi ◽  
Joo-Yeon Yoo
Keyword(s):  
Negative Feedback ◽  
Promoter Activity ◽  
Expression Patterns ◽  
Antiviral Response ◽  
26S Proteasome ◽  
Poly I:C ◽  
Wild Type ◽  
Conjugation System ◽  
Negative Feedback Regulation ◽  
Enhanced Production

ABSTRACT RIG-I senses intracellular virus-specific nucleic acid structures and initiates an antiviral response that induces interferon (IFN) production, which, in turn, activates the transcription of RIG-I to increase RIG-I protein levels. Upon intracellular poly(I:C) stimulation, however, the levels of RIG-I protein did not correlate with the expression patterns of RIG-I transcripts. When the ISG15 conjugation system was overexpressed, ISG15 was conjugated to RIG-I and cellular levels of the unconjugated form of RIG-I decreased. The ISGylation of RIG-I reduced levels of both basal and virus-induced IFN promoter activity. Levels of unconjugated RIG-I also decreased when 26S proteasome activity was blocked by treatment with MG132, ALLN, or Lactacystin. In the presence of MG132, ISG15 conjugation to RIG-I increased, and hence, the unconjugated form of RIG-I was reduced. In Ube1L−/− cells, which lack the ability to conjugate ISG15, basal levels of both RIG-I protein and transcripts were increased compared to those in wild-type cells. As a result, enhanced production of ISGs and enhanced IFN promoter activity in Ube1L−/− cells were observed, and the phenotype was restored to that of wild-type cells by the overexpression of Ube1L. Based on these results, we propose a novel negative feedback loop which adjusts the strength of the RIG-I-mediated antiviral response and IFN production through the regulation of RIG-I protein by IFN-induced ISG15 conjugation.

Аминогликозидфосфотрансфераза AphSR2 Streptomyces rimosus ATCC 10970: зависимость устойчивости к антибиотикам от серин-треониновых протеинкиназ PkSR1 и PkSR2

2020 ◽  
Vol 56 (1) ◽  
pp. 119-124
Author(s):  
Н. Н. Рудакова ◽  
М. Г. Алексеева ◽  
Н. В. Захаревич ◽  
Д. А. Мавлетова ◽  
В. Н. Даниленко
Keyword(s):  
Streptomyces Rimosus
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