Human cytomegalovirus induces alteration of β-actin mRNA and microfilaments in human embryo fibroblast cells

2004 ◽  
Vol 5 (6) ◽  
pp. 733-737 ◽  
Author(s):  
Mao-fang Lin ◽  
Guo-qing Wei ◽  
He Huang ◽  
Zhen Cai
Keyword(s):  
Human Cytomegalovirus ◽  
Human Embryo ◽  
Fibroblast Cells ◽  
Actin Mrna

scholarly journals The observation of time-course cytopathologic changes in human embryo lung fibroblast cells infected with human cytomegalovirus.

1996 ◽  
Vol 35 (1) ◽  
pp. 26-31
Author(s):  
Takako TAKEUCHI ◽  
Hiroaki TAKEUCHI ◽  
Lili KOU ◽  
Hisaaki SHINOHARA ◽  
Takako YAMAMOTO ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Human Embryo ◽  
Time Course ◽  
Lung Fibroblast ◽  
Fibroblast Cells

scholarly journals Two Sp1/Sp3 Binding Sites in the Major Immediate-Early Proximal Enhancer of Human Cytomegalovirus Have a Significant Role in Viral Replication

2005 ◽  
Vol 79 (15) ◽  
pp. 9597-9607 ◽  
Author(s):  
Hiroki Isomura ◽  
Mark F. Stinski ◽  
Ayumi Kudoh ◽  
Tohru Daikoku ◽  
Noriko Shirata ◽  
...  
Keyword(s):  
Viral Replication ◽  
Human Cytomegalovirus ◽  
Significant Role ◽  
Binding Sites ◽  
Human Fibroblast ◽  
Immediate Early ◽  
Fibroblast Cells ◽  
Recombinant Viruses ◽  
Human Fibroblast Cells ◽  
Hcmv Replication

ABSTRACT We previously demonstrated that the major immediate early (MIE) proximal enhancer containing one GC box and the TATA box containing promoter are minimal elements required for transcription and viral replication in human fibroblast cells (H. Isomura, T. Tsurumi, M. F. Stinski, J. Virol. 78:12788-12799, 2004). After infection, the level of Sp1 increased while Sp3 remained constant. Here we report that either Sp1 or Sp3 transcription factors bind to the GC boxes located at approximately positions −55 and −75 relative to the transcription start site (+1). Both the Sp1 and Sp3 binding sites have a positive and synergistic effect on the human cytomegalovirus (HCMV) major immediate-early (MIE) promoter. There was little to no change in MIE transcription or viral replication for recombinant viruses with one or the other Sp1 or Sp3 binding site mutated. In contrast, mutation of both the Sp1 and Sp3 binding sites caused inefficient MIE transcription and viral replication. These data indicate that the Sp1 and Sp3 binding sites have a significant role in HCMV replication in human fibroblast cells.

scholarly journals Role of the Proximal Enhancer of the Major Immediate-Early Promoter in Human Cytomegalovirus Replication

2004 ◽  
Vol 78 (23) ◽  
pp. 12788-12799 ◽  
Author(s):  
Hiroki Isomura ◽  
Tatsuya Tsurumi ◽  
Mark F. Stinski
Keyword(s):  
Virus Replication ◽  
Human Cytomegalovirus ◽  
Human Fibroblast ◽  
Recombinant Virus ◽  
Immediate Early ◽  
Wild Type Virus ◽  
Fibroblast Cells ◽  
Viral Protein Synthesis ◽  
Enhancer Element ◽  
Human Fibroblast Cells

ABSTRACT The human cytomegalovirus (CMV) enhancer has a distal component (positions −550 to −300) and a proximal component (−300 to −39) relative to the transcription start site (+1) of the major immediate-early (MIE) promoter. Without the distal enhancer, human CMV replicates slower and has a small-plaque phenotype. We determined the sequence requirements of the proximal enhancer by making 5′-end deletions to positions −223, −173, −116, −67, and −39. Even though recombinant virus with the proximal enhancer deleted to −39 has the minimal TATA box-containing MIE promoter element, it cannot replicate independently in human fibroblast cells. Recombinant virus with a deletion to −67 has an Sp-1 transcription factor binding site which may represent a minimal enhancer element for recombinant virus replication in human fibroblast cells. Although recombinant virus with a deletion to −223 replicates to titers at least 100-fold less than that of the wild-type virus, it replicates to titers 8-fold higher than that of recombinant virus with a deletion to −173 and 20-fold higher than that of virus with a deletion to −67. Recombinant virus with a deletion to −173 replicates more efficiently than that with a deletion to −116. There was a direct correlation between the level of infectious virus replication and time after infection, amount of MIE gene transcription, MIE and early viral protein synthesis, and viral DNA synthesis. The extent of the proximal enhancer determines the efficiency of viral replication.

Biphasic effect of cadmium on cell proliferation in human embryo lung fibroblast cells and its molecular mechanism

2009 ◽  
Vol 23 (6) ◽  
pp. 973-978 ◽  
Author(s):  
Gaofeng Jiang ◽  
Weixia Duan ◽  
Lei Xu ◽  
Shizhen Song ◽  
Changcai Zhu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Molecular Mechanism ◽  
Human Embryo ◽  
Lung Fibroblast ◽  
Fibroblast Cells ◽  
Biphasic Effect

Cell-cycle-dependent localization of human cytomegalovirus UL83 phosphoprotein in the nucleolus and modulation of viral gene expression in human embryo fibroblasts in vitro

2011 ◽  
Vol 112 (1) ◽  
pp. 307-317 ◽  
Author(s):  
Maria-Cristina Arcangeletti ◽  
Isabella Rodighiero ◽  
Prisco Mirandola ◽  
Flora De Conto ◽  
Silvia Covan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Human Cytomegalovirus ◽  
Human Embryo ◽  
Viral Gene Expression ◽  
Viral Gene ◽  
Embryo Fibroblasts ◽  
Cycle Dependent

scholarly journals Human cytomegalovirus infection modulates DNA base excision repair in fibroblast cells

Virology ◽  
2006 ◽  
Vol 348 (2) ◽  
pp. 389-397 ◽  
Author(s):  
Toril Ranneberg-Nilsen ◽  
Magnar Bjørås ◽  
Luisa Luna ◽  
Ragnhild Slettebakk ◽  
Hege Avsnes Dale ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Base Excision Repair ◽  
Cytomegalovirus Infection ◽  
Excision Repair ◽  
Fibroblast Cells ◽  
Dna Base Excision Repair ◽  
Dna Base ◽  
Human Cytomegalovirus Infection ◽  
Base Excision
Sign in / Sign up

Export Citation Format

Share Document